Goodies & Tip
Nan's Nook : Archives : Misc Tek : 9er Tek
Soak your grain for 24 hrs before sterilizing. This will "hatch" the encased spores so that they'll be susceptible to sterilizing. Be sure to get a good brand of organic rye grain from a health food store or co-op. Arrowhead is very good and doesn't always require soaking, but it's a good practice anyway.
45 min. to 1 hr. at 15 PSI works well for sterilizing Rye grain.
Instead of throwing them out, some experiments were tried:
A regular case knife was wrapped with Al foil, boiled
for 15 minutes, and placed (still wrapped) into the transfer chamber.
The stalled jar is sprayed with lysol, wiped down and put in the chamber.
A box of Saran wrap is also sprayed, wiped, and put in the chamber.
After the air in the chamber has turned over a few times, the jar is inverted
and the cake is birthed. The case knife is drawn from the Al
foil sheath and used to cut away most of the uncolonized substrate.
Then a length of Saran wrap is pulled from the roll and immediately placed
on the exposed substrate. Press the plastic wrap down gently to contact
the cake. Place the cake in a reasonably clean environment to finish
colonizing. Typically the cake puts on a burst of growth
and colonizes the remaining substrate under the plastic wrap in just a
few days. This process has worked 3 times in a row now, so it wasn't
just some lucky fluke. A transfer box with filtered air was
used and is probably necessary for this tek to succeed.
P.S. This process has worked well many times since
the above was written. The yields are somewhat less than a
perfect run, but that's to be expected. This technique has
also worked well when perfomed in front of the output from a HEPA
filter. In the case of "wet spot" bacteria or yeast, the contaminants
are mostly cut away and the rest die out (apparently) because of the change
in environment. These are the only 2 contaminants that I've seen
that will halt the growth, but not over run the existing mycelium.
They will co-exist for a long time.
There are some filters made for floor register vents (available at Walmart) which make great tops for the jars. They greatly improve the air exchange and cut down (or eliminate) stalled jars. The ones tested are "AmericanAirFilter tm". They are a non-woven material like a very fine Scotchbrite "scrubbie."
Just cut into pieces to fit the jars and screw the band on over them. One or two layers work well. It is recommended that you cover the top loosely with Al foil during sterilization and incubation.
This is an alcohol based emollient
clear gel that sterilizes, then needs no rinsing or drying. Gentle
on hands and dries clean. (also at Walmart)