Nan's Spore Print/Syringe Tek
Nan's Nook : Archives : Spores : Spore Printing
The trick to this tek is to take a print over water in a glass jar, blend the print with a wetting/suspension agent, and dilute the resulting spore suspension in sterile distilled water in order to make up to 2 liters of spore inoculum per print.
The handling of the mushroom cap and the printing process is quite well described in 9er Tek. Please read: 9er Tek Spore Printing and 9er Tek Blender Jar pages before proceeding. We will be making a switch, we will be taking prints in the blender jar using the procedure detailed in the 9er Spore Printing.
The blender jar is filled to about 1/2 way with distilled water. To this water is added either: one tablespoon of non-cidal (non spermicidal or bactericide) sex lube, such as ForPlayTM from www.trimensa.com, or AstroglideTM from http://www.biofilm.com. Alternately, one drop of regular unscented JetDryTM dishwashing rinse agent may be added per 100mls of print water (per Anno). The prepared blender jar is then pressure sterilized with the wire basket to hold the cap and the foil cover (the blade assembly is wrapped and sterilized separately) for 20 mins at 15lbs.
Once the print is taken in the jar over the water, the mushroom cap and basket assembly is removed and replaced with the sterile blender blade and gasket. The print is then blended for 30 seconds at a time for 3-4 periods with a short break in between. The blender jar is then returned to the glovebox where it is poured into a sterile quart jar, topped off with sterile water (to dilute the spore suspension), capped, and shaken.
If the print is very dark, 2 liters or more of spore solution can be produced through dilution. Spores processed in this fashion remain suspended longer, do not clump, and do not stick to the plastic syringe barrels. One print can make up to 200 syringes...
Spore Printing : Spores : Archive Main : The Nook
|Posted by: Nue Mar 16 03, 02:13 AM GMT|
| The way things are going in America we all need to learn to be self sufficient with our mushroom teks. Printing, cloning and syringe teks must be learned.
By adding a suspension agent to the spore water a couple shakes of the syringe will keep the spores well dispersed during innoculation. The pic below shows two pellets of "crosslinked polyacrylamide",cpa,tree moist, before and after soaking in water. After soaking in water overnight I put about a handful in two cups of water and blended at high speed for a minute. I then sterilized the solution in a pressure cooker. After cooled I sucked up about a cc of the liquid into a 9cc full spore syringe and shake to mix the water and cpa.
PF used something in his syringes. Drying a cc left a residue that became slippery to the touch with addition of a drop of water. Just like cpa.
|Posted by: Nanook Mar 16 03, 02:22 AM GMT|
| When blended do you get a uniform solution or does the polyacrylamide remain somewhat clumpy?
What do you think of the performance as a suspension agent...
And finally... Where have you been and what took you so long getting here ???
First post I have seen. Welcome Home Nue
|Posted by: HapplyDeranged Mar 16 03, 02:24 AM GMT|
|try jet dry...|
|Posted by: Mycota Mar 16 03, 02:27 AM GMT|
|Posted by: Molester Mar 16 03, 02:41 AM GMT|
| I bought some jet dry the other day, it says "now with baking soda" doesn't matter eh? Will that affect anything other than the pH of the suspension? I've been using a small pinch of agar, which works, but takes a while to suck into the syringe.
*sorry to borrow your thread, nue-I'd like to hear more about that tree stuff
|Posted by: HapplyDeranged Mar 16 03, 02:43 AM GMT|
|Posted by: Nue Mar 16 03, 03:01 AM GMT|
| In response to your question about "clumping". No, it blends to a perfectly smooth, slippery liquid. No problem with flow. I dilute it down quite a bit. It seems to keep the spores suspended indefinetly.
What took me so long? Well.....when I posted your name in a paragraph the other day on Mycotopia and got a Profanity error message that was it for me. And Hippie is gone. And I can swear there are cops posting suspicious subjects there. Maybe because of the link to PF. Not to spread rumors, just my gut feelings. Yeah I missed your input Nan. You are quite a man and have helped me with many things. See you around, huh?
|Posted by: ninja Mar 16 03, 06:15 AM GMT|
|Just a tid bit of info on syringes that we probably already know. With new syringes, ( at least with BD brand) There is a coat of silicon grease on the plunger. This makes the spores you are collecting congregate there. I have found that if you happen to be working with a piece of a print or a light print, a great mass of the spores become trapped here and cut your ability to use a lot of the viable supply. Good idea before you use them to wipe this from the plunger, resterilize etc.|
|Posted by: dcyans Mar 16 03, 09:44 AM GMT|
|Posted by: Magash Mar 16 03, 11:45 AM GMT|
|Posted by: DirtyWOP Mar 16 03, 11:54 AM GMT|
|What was it now.....1 drop per 100 mL I think?|
|Posted by: Fungusmaximus Mar 16 03, 12:32 PM GMT|
1-2 drops per 100ml yes thats it wop, I use it too
Ionic wetting agents can be used for a ton of other things too! Got some polyurethane spilled on your carpet by a clumsy girlfriend?
Jet dry will keep it from hardening and setting up. Super sticky adhesive removed with afew drops and a rag. Clean your wbs real good by adding a squirt in the soaking bowl, less soaking and rinsing.
Agar sticking to the transfer tools an ultra thin layer of jet dry will cure that right up. Add some to nans sanitizer spray to keep mold spores from sticking... It goes on ...
|Posted by: Nue Mar 16 03, 08:58 PM GMT|
|"The blue stuff" sure sounds a hell of alot easier.|
|Posted by: Nanook Mar 17 03, 04:02 AM GMT|
|I agree, but they are both getting archived, I have been putting focus on the print teks|
|Posted by: HapplyDeranged Mar 18 03, 01:19 AM GMT|
|you really cant over do it with the jet dry...about a dime size droplet(s) in the bottom of a coffie mug for about 5-6 syringes...i bet you could use 50/50 and it would still work.|
|Posted by: trinity Mar 25 03, 02:21 PM GMT|
| my friend likes the EQ's. he looked all over and all he can find is jet-dry with baking soda, lemon, or citrus(orange)-so he got the baking soda kind. is this ok for the syringe teks or does he need to find the other kind?
does he need the jet dry at all because he did a GT print in plain water with fish hook, will that be OK?
|Posted by: MyInsanityTrip Mar 25 03, 06:03 PM GMT|
| My pal, who's no expert, tells me that just scraping spores from a print into plain water is A-ok. He tells me, and keep in mind he's a shakey source of knowledge, that the jet dry is just used as like a seperating agent, to help disperse the spores and increase the amount of syringes made from each print.
Is your pal just letting your cap drop spores into water? I'm not sure if that's a healthy tek or not, maybe I'll ask around.
|Posted by: trinity Mar 25 03, 06:47 PM GMT|
|yeah he's just letting the spores drop into plain water. i really don't know what the jet dry is for either thats why i'm asking. my freind only needs to make like 2-3 syringes per print. anybody know if he needs jet dry?|
|Posted by: Iggy Mar 25 03, 07:51 PM GMT|
|This was disscused not to long ago. Jet Dry is simply a suspention agent to keep your spores off the sides of the syringe and keep them from clumping. Make sure you get the blue bottle Just a drop will go a long way. Oh and I hope that is sterile water (boiled or "sodium free" bottled water) in a air tight print chamber (widemouth pint jar with the lid on?) or maybe in a glovebox? Hope this helps you understand more That link is full of great info on the jetdry|
|Posted by: dcyans Mar 25 03, 08:39 PM GMT|
|jet-dry with baking soda will work just fine|
|Posted by: Nue Mar 26 03, 04:44 AM GMT|
|I put some jet dry, a few drops in 4 oz. of water and let a cap drop spores on the water. Just jiggling the water a little let the spores on the surface of the water fall like purple dye into the water. I'm sold.|
|Posted by: HapplyDeranged Mar 26 03, 12:25 PM GMT|
|jet dry is the only way to fly when makeing your own syringes....the spores are evenly spread trought the H2O so when you make your syringes you will have the same amount of spores in each syringe.....i think it also helps spread prints out 4-5 times that of a normal syringe tek...|
OK I was asked about this in chat...
Sanitizing caps for printing. Here is what I have worked out.
You want 25 ppm free iodine from either a dilute Betadine or generic Iodophor sanitizer.
25 ppm is approx 1 cc of iodophore in 500 cc water.
When sterilizing equipment for spore printing I place a glass with 250 cc of deionzed water and a cotton ball (cover with foil) in the pc. I also sterilize a foil wrapped pair of forceps.
Before I remove the mushroom cap for printing I uncap the glass with water and inject 1/2 cc of iodophore. I grab the wet cotton ball with the forceps, stir the sanitizer in, and use the cotton ball to wash the top of the cap after it is hooked and cut for printing.
Do not mess with the gills or turn the cap over for any reason. Wash only the top of the cap, gently, and allow excess sanitizer to drip off, then place in the mount for printing (some people place caps directly on the print media, I mount them in a wire basket or platform and suspend them just above the print media)
Printing caps need moisture to print well. One reason I like printing over water is the humidity stays high. You can also cover a printing cap with a sterile filter paper or thin cotton cloth (wrap materials in foil and sterilize in the pc). Use the cotton ball and forceps to moisten: stuff dries out fast (esp in a ventilated box). Apply sanitizer solution (be very careful not to drip), even directly to the top of the cap, in order to keep it clean and hydrated for extended printing.
A large healthy cap harvested at the beginning of the spore drop will print for days if it is properly set up and maintained and will produce several heavy prints - Nan
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