Nan's Nook : Archives : Misc Tek : FAQ : Sanitizing : Sanitizers                            Also See: Fogger System
Posted by: jondo May 13 03, 01:00 PM GMT
a friend is setting up a rubbermaid fruiting chamber, will spraying down the entire inside sterilize it, what other methods can be used to sterilize a large fruiting chamber

Posted by: jondo May 13 03, 02:52 PM GMT
i forgot to say spray with Lysol

Posted by: steveoi812 May 13 03, 02:55 PM GMT
I would say that you could probably use a little h202 with your perlite. Might keep things cleaner longer. Make sure there are no fumes left in the bin before you put cakes in there. I have had cakes killed from bleach fumes. Just make sure everything is nice and clean. Cakes are fairly contam resistant, imo... smile.gif

Posted by: kingkc May 19 03, 08:28 PM GMT
Just clean it out real well and you will have no problems.

Posted by: Fungusmaximus May 19 03, 10:17 PM GMT
Air is most important to keep contams at bay wink.gif IMO

Iodine in the Perlite lasts much longer than h202 and kills all, unlike peroxide that will only kill some spores. Iodine kills spores, and the mold itself. wink.gif

Posted by: DirtyWOP May 19 03, 10:35 PM GMT
Usually I just wash mine between grows with hot soapy water, bake the perlite, give the bin a lite, fine, bleach mist and insert substrate......after that mist wild a mild peroxide should get many flushes

Posted by: Smerd May 23 03, 11:32 PM GMT
FM, I've wondered about iodine. How do you use it (i.e., just a drop or two?)? Can it harm cakes?

Posted by: Nanook May 28 03, 03:13 PM GMT
Any sanitizer will retard cakes. The idea being that if sanitizer solution comes into contact with a cake it retards contams more, giving the cake an edge when it recovers.

As for iodine in the fruiting chamber, it does not vaporize or leave fumes and as long as the cakes do not sit in contact with the solution directly the only harm a few drops in a fruiting chamber does is to the bacteria and mold contams wink.gif

Posted by: eleutheromania May 28 03, 09:59 PM GMT
Only a drop or two? That seems like a small amount, is there a good ratio of water for perlite to iodine? I don't mean to question the honourable nan bow.gif I just wanted to double check. smile.gif

Posted by: Nanook May 28 03, 10:09 PM GMT
2-3 drops of tincture in a liter of clean water to soak, rinse, and top off perlite is fine. Remember no standing water in the grow chamber if you use Perlite.

This solution can be used to wash down mycelial tissue prior to cloning, and caps before spore printing (they love a drink). Allow to dry before working with treated tissue.

For cactus grafting I use a stronger solution. Like a dilute ice tea color obtained with several drops of tincture in 12 ounces of clean water.

Other iodine based sanitizers work well: Betadine (Provodone-Iodine 10%) as well as Iodophor sanitizers used in the brewing and bottling industry. For most purposes if you can just see a tint, you have enough free iodine in solution to be an effective sanitizer with a 20 minute contact time.

OK I was asked about this in chat... Sanitizing caps for printing. Here is what I have worked out.

You want 25 ppm free iodine from either a dilute tincture, Betadine or generic Iodophor sanitizer.

25 ppm is approx 1 cc of Iodophore in 500 cc water.

When sterilizing equipment for spore printing I place a glass with 250 cc of deionzed water and a cotton ball (cover with foil) in the pc. I also sterilize a foil wrapped pair of forceps.

Before I remove the mushroom cap for printing I uncap the glass with water and inject 1/2 cc of iodophore. I grab the wet cotton ball with the forceps, stir the sanitizer in, and use the cotton ball to wash the top of the cap after it is hooked and cut for printing.

Do not mess with the gills or turn the cap over for any reason. Wash only the top of the cap, gently, and allow excess sanitizer to drip off, then place in the mount for printing (some people place caps directly on the print media, I mount them in a wire basket or platform and suspend them just above the print media)

Printing caps need moisture to print well. One reason I like printing over water is the humidity stays high. You can also cover a printing cap with a sterile filter paper or thin cotton cloth (wrap materials in foil and sterilize in the pc). Use the cotton ball and forceps to moisten: stuff dries out fast (esp in a ventilated box). Apply sanitizer solution (be very careful not to drip), even directly to the top of the cap, in order to keep it clean and hydrated for extended printing.

A large healthy cap harvested at the beginning of the spore drop will print for days if it is properly set up and maintained and will produce several heavy prints

Grow Chambers : Clean Water : Sanitizers : Sanitizing : Shroom Glossary