Amount of Inoculum per jar
|Posted by: steveoi812 Jan 13 03, 07:53 PM GMT|
| How many ccs of spore water should I use in a pint of your wbs mycota? What is the max I can use without throwing off the correct level of moisture in the bird seed? Keep in mind I am only doing pints...Thanks
|Posted by: skeight976 Jan 13 03, 08:19 PM GMT|
|yeah ive been adding one CC per umm Little jar(sorry for got the name) but it doesnt seem like thats enough. so lets get some help here|
|Posted by: dcyans Jan 13 03, 08:34 PM GMT|
|if i were you i would do the karo tek ( i can do 4 half pint jars with 1 syringe) then incubate for 10 days or so. the mycelium/karo mix grows very quickly ( i've seen growth in 24 hours) so contams don't have as good a chance to get started. 1 full syringe per quart jar, you could get maybe 10 qts. innoculated. karo tek=1syringe=40 or so quart jars or 80 pint jars and they will grow FAST!!!! Polyfil pc's just fine.|
|Posted by: steveoi812 Jan 13 03, 08:36 PM GMT|
|hmm so 10-12 ccs of karo water wont be enough to throw off a quart jar of wild bird seed? S I guess I will just do 5ccs per pint then. Thanks|
|Posted by: skeight976 Jan 13 03, 08:49 PM GMT|
| NO im talking about verm and rice and that kinda basic stuff
|Posted by: steveoi812 Jan 13 03, 08:54 PM GMT|
| skeight you can use more spore water to your jars but I think you would probably get better results (as in faster) if you would try and do the karo tek
|Posted by: dcyans Jan 13 03, 09:00 PM GMT|
|you will save germination time using karo tek for one thing. mycelium grows almost immediately (within 24hrs.) from Karo inoculations.|
|Posted by: HapplyDeranged Jan 13 03, 09:10 PM GMT|
|or just put the syringe in your incubator for 2-3 days prior to use...that should germinate them....syring must be used asap or your germinated spores will die due to lack of nutes|
|Posted by: steveoi812 Jan 13 03, 09:11 PM GMT|
|Damn I've never heard of that before. Anyone else ever heard of that? Have you done it happly?? Then I start to think and I ask myself why risk it when they will germinate in 2 or 3 days anyways...Still sounds interesting though...|
|Posted by: Mycota Jan 13 03, 09:52 PM GMT|
| There is no exact number of CC's (liquid) to squirt into a jar. Just so long as you do not inject to much fluid, so to throw the moisture content OUT OF BALANCE.
The object is to get some viable spore into the jar & mix inside. So, just shake the hell out of a syringe, before you inject. Then only squirt enough fluid in the jar, against the glass, so that you see the fluid run from the top of the jar, to the bottom.
I use two ....1/4 inch.......holes in my lids, offset about 1.5 ior 2 inches.
I inject into one of those holes & swivel the syringe, without withdrawing it. I put a tiny squirt on four (4) sides & 1 down the center. Just enough fluid, I'm sure ut reaches the bottom of the jar.
Then, withdraw the syringe & tape over the lid hole I injected through. That seals the lid (where internal filter material is peirced) & leaves one hole for gas exchange.
Here is what 2 squirts looks like, six days after injection of a multi/spore innoc. There are two other on the other side of the jar, that look just the same.
|Posted by: steveoi812 Jan 13 03, 10:03 PM GMT|
|Posted by: HapplyDeranged Jan 13 03, 10:26 PM GMT|
|no but it will work....got some pre germinated innoculant and put 4 cc in 1 pf cake....wow.....like wildfire im beting it will be fully colinized in 9 days.|
|Posted by: Nanook Jan 13 03, 10:34 PM GMT|
| You can't guarantee the syringe will germinate. My homemade syringes in sterile water incubate just fine for a month without germinating. I know because I tested them... and they were still viable when shot into jars.
I would use no less then 4 cc's per qt
You can use up to 10cc's per quart, but you have to dial the moisture content of the substrate down to compensate for the extra liquid. If the substrate does not absorb the liquid within a few hours, and it stays wet, it's a fair chance wet spot bacteria will start and kill the inoculum.
I shoot em by eye, very much the way Mycota shows... takes about 7 cc per jar to shoot em good See: My Grain Tek
Pro's thread on Rye : Shroom Glossary