Magic In A Bottle
|Posted by: Mycota Dec 06 02, 02:09 AM GMT|
| Right up front. Liquid culture is a CONTAM magnet. If you do not have a GOOD glove box, hepa filter or flow hood........& good tek..............PASS.
This is a liquid culture of EQ myc, in a quart jar w/filter top & injection port n like day 5.
What you see in the bottom is ~ green plate glass shards. They are there -- to cut up the thick mass of myc -- when the puppy is shaken. Otherwise, the myc will PLUG a syringe, when you try to suck it out.
This was done with "light dry malt", a teaspoon to as much water, as you see in the quart jar. PC'ed 20 minutes @ 15 lbs.
|Posted by: OneDiaDem Dec 06 02, 03:57 AM GMT|
|Love liquid culture, it is the bomb!!! Just got my pans going tonite!|
|Posted by: DirtyWOP Dec 06 02, 07:46 AM GMT|
|Whats up with that filter?|
|Posted by: HapplyDeranged Dec 06 02, 12:15 PM GMT|
|its only a contam magnet if your not clever..... just use a self healing injection port on the lid. NEVER expose it to the elements and sterilize the needle havent had a contam yet. 1/2 tbl spoon of Brown Rice Syrup to a 1/2 pint pc'd for 15 min at 15 psi. 1cc of spore medium or a transfer of culture to culture and your in buisness. mycota maby you can shed some light on the subject your a pro with that additive|
|Posted by: plinkerdink420 Dec 06 02, 04:38 PM GMT|
|i love rice syrup... i used to use it alot for liquid cultures and as a light additive to my rice...|
|Posted by: Mycota Dec 06 02, 04:57 PM GMT|
I have used & posted about injector ports, long ago - in another forum. Not long after doing so, a vendor (apperantly) copied the idea & began selling them. He simply added a 0.30 cent rubber septum plug to a 0.25 cent plastic lid & charges $5 each for them.
There is not even a need for that. All you do is drill a small hole in a plastic jar lid (Kerr brand 8 for $3 @ most grocery stores - canning supplies) & screw a small stainless steel screw (10 cents ea @ hardware stores) into it.
Just make sure a syringe needle will fit through the hole. Use either light dry malt, karo, brown rice syrup w/water as a nutrient. PC 15 - 20 minutes, let it cool, innoc w/spore syringe through hole, replace screw & you end up with a liquid culture. Remove screw to withdraw culture w/syringe.
It is wise to place some glass shards inside the culture jar. Because it can get THICK & plug a syringe, when you try to suck it out. The shards will help cut the myc up, if you agitate the jar w/them inside.
As for brown rice syrup (BRS), I think I was the first to use it, for liquid culture and/also as an additive into water spawn seed/grain is soaked in. Just to add complex sugars, carbs, protein & more nutes to the spawm seed/grain. No big deal.
|Posted by: DirtyWOP Dec 07 02, 08:38 AM GMT|
| I don't understand.....
Why a steel screw? How is that a self-healing injection port.....?
I've made liquid cultures with a certain type of ear plug, and pencil erasor and they both work. You just have to have a sharp syringe, and insert it so it doesn't clog......
|Posted by: EvilShroomer Dec 07 02, 08:48 PM GMT|
|where can i read up on this liquid culture , that kewl , it just like water with mycelium in it, hah , id love to have tons of jars just with that , and then see pin in it , haha , and then mushrooms , hah that be kewl, plus it look like , it better to be cased...|
|Posted by: ShroomVator Dec 08 02, 11:11 PM GMT|
| Try munchin' on the archives for a bit.
|Posted by: HapplyDeranged Dec 09 02, 01:27 AM GMT|
| if you think about it the srew plugs the hole....just find a real small screw, drill a hole a tad bit smaller than diameter of screw and put the screw in before/after innoculation......cheap and easy.
on another note anyone know a retailer for the septum plug?? did a google search and came up empty handed after 2 hrs of searching.
Perfect Lid : More Injector Lids : Shroom Glossary