Quasi/clean Room Counter Top G2g

Posted by: Mycota

Nan's Nook : Archives : Cloning  
A foaf had a few colonized WBS Jars in the fridge & noticed they were getting dehydrated. So, he decided to G2G then, to keep them going.

 

Posted: Feb 19 03, 02:58 PM GMT  
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Posted: Feb 19 03, 02:59 PM GMT  
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Posted: Feb 19 03, 03:01 PM GMT  
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Posted: Feb 19 03, 03:03 PM GMT  
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Posted: Feb 19 03, 03:04 PM GMT  
Screw the lid on & shake, to distribute the colonized WBS inside the fresh WBS jar & incubate @ 80 - 84F. Mycota wink.gif



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Also see: WBS TEK : Clean Rooms

Posted: Feb 19 03, 03:31 PM GMT  
Now that's purty!!! A perfect picture of my goal! (rubs hands together drool.gif drool.gif )
Posted: Feb 19 03, 07:20 PM GMT  
how much $ is it for WBS?
do they have it at wal-mart? laugh.gif

can i use it without a pc? i can boil with a really tight fitting lid for 4-5 hours if necessary, after soaking. i just can't afford a pc right now.
Posted: Feb 19 03, 07:54 PM GMT  
QUOTE
how much $ is it for WBS?

very cheap. i've seen it as low as 29 cents per pound at farm supply stores.
buy the cheap seed with less sunflower seeds and no fungicide.

QUOTE
do they have it at wal-mart?

yes.

QUOTE
can i use it without a pc?

no.

QUOTE
i can boil with a really tight fitting lid for 4-5 hours if necessary, after soaking. i just can't afford a pc right now.

save your money and buy when you can afford it.
Posted: Feb 19 03, 09:52 PM GMT  
5lbs 1 dollar. The best seed I have ever purchased. Huge grain, no stickin, no dirty shit. Rinse once drain and go!
Posted: Feb 21 03, 05:41 PM GMT  
QUOTE (eatyualive @ Feb 21 03, 09:24 PM GMT)
ingenius!!!!

mycota, in an open environment such as the one shown, would it be helpful to do the transfers as quick as possible just to be safe?


- a friend does open air transfers very quick and this is why i ask, and also to help any newbies in need....

It's not about QUICK

It is about first spaying a room with lysol, then filtering the room first, with any room type hepa filter running full blast. Then, wearing clean (fresh laundry) clothes, wearing a shower cap over your hair, wearing a face mask over nose & mouth, spaying lysol, shutting the hepa down (SO AIR IS CALM & THERE IS NO AIR MOVEMENT), then spaying your hands/arms & counter top with lysol & doing it CALM, least amount of movement & in a steady even fashion.

Part of the trick is to lysol spray the outside of the jars & wipe them down, first before doing this. Also, lysol spray a paper towel & if you set the spoon down, set it on that sprayed paper towel.

Between doing new jars, wipe spoon with a lysol sprayed paper towel.

This room is small (smaller is better - less air - less contam's), & ceiling - walls & floor plastic wrapped - air tight. My foaf has done 100's of jars just like this - with about a 1% contam rate. Now, he does it - in front of a laminar flow hood & contam rate is almost ZERO.

Mycota wink.gif
Posted: Feb 21 03, 10:30 PM GMT  
just like one big people sized glove box smile.gif
Posted: Feb 21 03, 11:18 PM GMT  
QUOTE (zoopsie @ Feb 22 03, 04:30 AM GMT)
just like one big people sized glove box smile.gif

Exactly. I like ya - already - Zoopsie. Mycota (6T)
  Posted: Feb 22 03, 05:13 AM GMT  
Mycota,

How are thoes fancy lids working out? Was that three layers of Tyvek? What was the spacing distance? What is the aproximate diameter of the filter area? Is it safe to inject through the rubber body? Had any problems sterilizing them over and over? Thanks.

I'm nobody.

BTW - How are you recoving from your trip to the emergency room? ohmy.gif
Posted: Feb 22 03, 01:03 PM GMT  
QUOTE (nobody @ Feb 22 03, 11:13 AM GMT)
Mycota,

How are thoes fancy lids working out? Was that three layers of Tyvek? What was the spacing distance? What is the aproximate diameter of the filter area? Is it safe to inject through the rubber body? Had any problems sterilizing them over and over? Thanks.

I'm nobody.

BTW - How are you recoving from your trip to the emergency room? ohmy.gif

The lid in the picture above is intended for G2G only & has no Injection Port. Hole is 1/2 inch, spacing is 1/16th apart & yes - 3 layers of Tyveck.

Limping, still, but getting better. Mycota (6T)

Clean Rooms : Cloning : Grain Teks : Misc Teks : Archives Main : Shroom Glossary

Oooops Just a Reminder!!

Posted by: Mycotia Nov 26 02, 02:34 AM GMT
Grain to Grain Transfers (G2G)

Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you donít have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place.

Remove the filter material from the colonized jar & dig up about ľ of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting itís filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet.

G2G transfer & shaking jars batters the mycelium. It takes it a day or 3 recuperate from that shock. There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.

Mycota


 

Posted by: DirtyWOP Dec 11 02, 01:27 PM GMT
I'm about to do a grain to grain transfer and want to inject some peroxide into the jars before I innoc......but I don't know how much to add. Will 1 cc suffice? Is this asking for contamination?


Posted by: Anno Dec 11 02, 01:42 PM GMT
Add 5 ml of 3% peroxide to a quart jar of grain and shake very well to distribute the peroxide over all kernels.


Posted by: DirtyWOP Dec 11 02, 01:46 PM GMT
thanks a bunch anno


 

6T Grain to Grain Transfers (G2G)

Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you dont have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ľ of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting its filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3 recuperate from that shock. There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.
 

Mycota (6T)

Remember ^ the ^ Reminder Jar?


Eatyualive's Lazy Mofo Bags

Posted by: eatyualive Feb 14 03, 10:58 AM GMT
here is an idea that I thought of. I just sterilized the working area as usual and sterilized the air with lysol. i crunched up a little of one of the colonized bags and opened the wbs quart. just poured a little of the mycelia in the wbs and then shake afterwards to get the mycel pieces distributed throughout the jar! this jar should colonize in a few more days and then it will go to dung!


Posted by: eatyualive Feb 14 03, 10:58 AM GMT
...birdseed spawned


Posted by: Fungusmaximus Feb 14 03, 07:47 PM GMT
use only cakes that are under 20 days old. the older they get the more chance of bacterial contamination. I got a load that I did the same thing with, only in a hepa box, and they all got wet spot from the cake sad.gif


Grain in open air is risky IMO, you are walking a fine line between fruits and contamination...IMHO wink.gif laugh.gif


Posted by: eatyualive Feb 16 03, 05:17 PM GMT
well it has been two days since inoculation( the bags were only 4 days old after colonizations). have about dime size growth from each spot of inoculation. lookin real good so far!


Posted by: Zoom Feb 16 03, 05:46 PM GMT
Hey Eatu...I tried putting 2 pf cakes in a ziplock bag
but they failed to recolonize. sad.gif
What are some common mistakes using this tek.
It looks very promising to me and would like to
get it down.


* Thanks Eatu..Read your reply on the other post. wink.gif


Posted by: OneDiaDem Feb 16 03, 05:58 PM GMT
I cannot stress being sterile enough. I just tossed major amounts of wbs that were almost at 100%. Funny thing was, the only thing I did not do, was have a filter on the jars, because I took them straight from the pc into the glovebox, where they were innoculated with agar, and left to colonize (inside the glovebox). Been a very frustrating month for me. So, starting over. The only thing that did not go bad was my h2o2 agar. This is a mean mold I have goin here. Dont be lax with your sterile teks. I wasnt, and still ended up tossing quite alot of work. cry.gif


Posted by: Zoom Feb 16 03, 06:05 PM GMT
Sorry to hear that OneDiaDem.
That does suck. Hope you next
batch does better for you.


Posted by: OneDiaDem Feb 16 03, 06:13 PM GMT
Back to Mycota 101. That "me do" stuff gets me into trouble every time. tongue.gif


Posted by: eatyualive Feb 18 03, 01:26 PM GMT
well it has been a few days and the birdseed is almost 90% colonized. just shook em to get the extra 10% colonized. interesting thing was that the mycelia was a rock hard solid mass already in a few days time and almost didn't break apart!


Posted by: eatyualive Feb 21 03, 04:34 PM GMT
fully colonized wbs 4 days from the bag tek

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