Cropping Consistent Yields.....
|Posted by: DirtyWOP Apr 29 03, 11:28 AM GMT|
| So you want consistency in your yields?
Tired of the random results?
I think some culture trials are in order.....
but let me warn you....this is a lot of work.
Step one is selecting a spore race. It's best to avoid races that people complain about, for they are likely to produce to more illegitimate mating than others, and will make the work towards a known fruiting strain tougher.
Now that you have your print.....you'll need to lightly streak 5-10 media-filled Petri plates with spores. Do this by flicking the print over the plates, rather than swabbing in one spot, for this will make discerning strains easier.
Now let me assure you that once the plates are growing, there are SEVERAL DOZEN different strains, ie...paired dikaryons, growing in each plate. But just because two hyphae happened to fuse and form a dikaryotic strain, doesn't mean the pair is legitimate, ie. a fruiter.
In each plate, the different strains ought to sort themselves out to certain degree, and you should be able to see the different strains segregate into small sectors of differing physical appearance and growth rate. Now it's time for transfers....
Now......your gonna need a lot of disposable petris, and a system of sorting them out and labeling them. Numbers and letters. Each fruiting strain, once pure (isolated), will have a unique set of preferred culture conditions. Our goal is to isolate as many as possible and test them for yield ability.
So what we do is prepare a few plates to culture from each plate of spores. Try to discern the differing sectors and transfer them to new plates. On the first transfer, sometimes it's unavoidable to get several strains in your wedge, but they will sort themselves out eventually and produce a pure strain. From all the spore-plates, try to make about 10 new plates, trying to get a diverse range of different types of growth, but avoiding all cottony sectors( only for cubensis, if working with pans, the cottony stuff is sometimes better).......
Continue to isolate differing strains by transferring new growth to new plates until you have 10 pure strains. Should take 6 or 7 plates per strain to get a true, pure strain. You know it's pure as soon as it stops sectoring.
Now there are two paths we can take to test for fruiting ability.
I'll detail the better, traditional way first. Take a wedge from each pure strain and transfer each to one quart jar of grain a piece. Don't forget to label. I have to note now that if your looking for consistent yields, it's best to have a fruiting procedure, in other words, grow the same way all the time. I recommend grain to compost....but anyway, find a way and stick to it. Once your grain is colonized, it's time to test for fruiting on your choice of fruiting substrate.
Spawn each quart of grain to two trays of your choice bulk substrate. You have to do AT LEAST two trays just for control, to avoid flukes. You will need a large fruiting chamber since you have twenty trays you need to fruit out. You need one large chamber b/c all the trays need to have identical environmental conditions.
Keep environmental parameters constant until the trays fruit. Note the characteristics of each strain and choose the best overall fruiter. There will be many factors involved, like whether it's a cluster fruiter, or not, or if it forms large fruits or lots of small ones, so if you want, choose more than one.
>>>>>The simpler way to test for a fruiting strain is to expose all your petris to light and see which fruits invitro. This method is fast, is less work, and easier, but it's not as good as the method above.
Now it's time for stock culturing. Go back to the isolates that performed the best on both test trays. Use that plate to transfer to a stock culture, usually made in test tubes....grow it out, and take it to the fridge. This is your master culture and can be used to produce spawn in the future.
This is the way to REALLY get it right. I don't really do this, because I'm not interested in producing huge reliable crops of psilocybe mushrooms.....but maybe that's what someone's been looking for, so there's the dilly for ya....
Also, P. cub is so easy to grow from spores, that this technique isn't really necessary. Even multispore cultures perform well b/c it's just an easy to fruit mushroom. But this isn't the case with almost every other mushroom. So strain selection is good to know if you want to produce any other mushroom.
Remember, contamination can devastate all your lab work and fuck your results........so stay as clean as possible during the procedures and use plenty of peroxide.....
have a nice day
|Posted by: killdannow Apr 29 03, 12:17 PM GMT|
|Archive material WOP. Good piece tho - satisfied my curiosity.|
|Posted by: dcyans Apr 29 03, 12:35 PM GMT|
|I'd also like add for storing these stock cultures for long periods of time is place a thin layer of sterile mineral oil on top of the mycelium once it has been established in a test tube nice read DirtyWOP|