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|Nan's Nook > Fungi > Q About Tyvek|
|Posted by: Dell500 May 11 03, 05:08 PM GMT|
| If using tyvek, and it's placed before metal lid is on, can syringe go through tyvek when innoculating?? Thanks.
Edit: Also, with WBS, should the sunflower seeds be removed or do they add nutes?? (Big pain in the arse to remove because there are so many lol), thanks
|Posted by: Dawgboy May 11 03, 06:05 PM GMT|
| It can go through, but you should seal it afterwards with tape.
The question about sunflower seeds gets asked a bit. Some people say that some of the nutes provided by them are quite useful, but that the myc might have a bit of trouble colonising them (and/or that the thick shell makes sterilising more difficult).
The easy way to get rid of them (if you want to) is to pour the the wbs into lots of water. I believe the sunflower seeds float while the rest sinks. I'd say get rid of them but others might disagree.
|Posted by: dcyans May 11 03, 07:29 PM GMT|
|When I soak my WBS I pick out the floaters and don't worry about the rest I've never had a problem I like to use a drop of hot glue to seal any innoculation sites|
|Posted by: maryxmas May 11 03, 08:26 PM GMT|
| what i do is rotate the lid a half turn over the tyvek and then innculate and then rotate the lid back a half turn. that way the innoc hole in the tyvek isnt in direct line with the hole in the lid so if anything does fall though it lands on the other side of the tyvek away from the hole.
|Posted by: Samsara May 11 03, 09:03 PM GMT|
|I have always punctured the tyvek when innoculating and just left the hole without sealing. I have never had any contams at that stage of growth (although I haven't done very many grow either). If you want to be on the safe side, use tape or hot glue like dcyans said. Good luck!|
|Posted by: mycofile May 12 03, 11:29 AM GMT|
| You won't get away with not sealing for very long. Well, you might, but you might as well not be using a filter if you are that lucky.
Sunflower seeds are argued often. I used to pick them all out just to be safe. Then I started picking out most of them to be somewhat safe. Then I just left them in and realized they were fine. They do contain a unique nutritional profile which is quite helpful to mycelium. The thick hull may make them a little more difficult to sterilize, but once sterilized, sterile is sterile whether its from a sunflower or a cow.... I suspect that it's the dark color that makes it easier to spot contams on before other grains, not that the contam originates with them, but it's just noticed there first.
They do float when mixed with water, and can be skimmed off or poured off. You'll have to do this many times, stirring as you go to remove them all from a big bag. Even at that you'll have to go through and pick some out by hand if you want all of them out. I say leave them be, and pc for 1.5 hours just to be sure. But, if you rinse your grains (which you should anyway, at some time before pcing) then it's easy enough to pour the majority of them off.
|Posted by: Camelot May 12 03, 11:42 PM GMT|
| I just picked up some WBS..."No Waste Select" (Stokes brand). It has 'sunflower chips'...(just the seed part...no hulls)...along with white millet, hulled millet, cracked corn, peanut pieces and red millet. It was about $4 for a 7.5 lb bag.
1. Is this WBS OK?
2. If so, has anyone considered either using this type or some other type without SF seeds and just adding 'shelled' SF seeds to regain the nutrient value?
|Posted by: mycofile May 13 03, 04:57 PM GMT|
| 1. Yes
2. No, the beauty of WBS is it's simplicity. Put it in a pot, soak it over night, rinse, strain, pc. Adding stuff to it takes away from it's simplicity. Also, I see no problems with sf seeds of any form. I think if people think they have problems caused by sf seeds, they have other problems that are being misdiagnosed. If you don't want to use sf seeds, fine, but I wouldn't worry about compensating for any lost nutrition.
|Posted by: trinity May 13 03, 05:17 PM GMT|
| my foaf just put WBS, then tyvek, then a ball of polyfil, then the dome lid, coffee filters, then foil. pc'd for 95 min. they're cooling in the glovebox, palenque liquid innoc. tomarrow.
poly fil because he has no hot glue gun to seal up the hole. hopin for the best
|Posted by: dcyans May 13 03, 07:38 PM GMT|
Couldn't agree more mycofile I've seen straight white millet in the pet store i'm sure that would work awesome too
|Posted by: Maineiac May 14 03, 02:15 AM GMT|
| I tried plain white millet from the natural food store. The mycelium RAVAGED the stuff. Moisture content is fickle though, took a few tries to get it down.
I'm trying to get a hold of some Tyvek but I've only found it in big ass rolls for way too much money. Where do you folks get yours?
|Posted by: Bobkat May 14 03, 02:27 AM GMT|
Mainieac you should try construction sights where some unsuspecting homebuilder is not paying attention or ask him if he can spare you some of the scrap.
|Posted by: ace May 14 03, 03:39 AM GMT|
| I wasn't sure of what you all meant when I heard Tyvek. Now I know. What I've used is this fabric that is like goratex. It's about a 400 count thread nylon with a rubberized backing. It can be PC'd and I've used the same piece 3 times PCing it and poking the hell out of it with those big 18 guage hypo's. The hypo hole just kind of seals back up. No shit. I bought a six inch by three foot length of it for about $4 I think it was at the local fabricworld. I've got to see some of this tyvek now.
edit:can't put a pic up on an edit I guess
|Posted by: ace May 14 03, 03:47 AM GMT|
|This is a pic of the fabric I was describing above.|
|Posted by: trinity May 14 03, 07:32 AM GMT|
|i just wanted to say that it is illegal to use TYVEK express mail envelopes from the post office if you are in the USA. repeat, do not use the TYVEK express mail envelopes from the post office. they are perfect for this application, but it is illegal to use them for anything else besides mail. that being said, my friend of a friend of a friend of a friend uses those, for eh, mailing stuff, yeah. they can be PC'd and they are free|
|Posted by: Twista May 14 03, 10:36 AM GMT|
my foaf ran into the same problem. Go to local staples if you have one. They sale 9"x16" envelopes. Around $15 for 100 and you can make at least 8 from one envelope if you cut it right. Tearing this stuff can be a pain and ultimately result in ill-shaped tyvek filters.
|Posted by: glasshoppa May 14 03, 11:17 AM GMT|
I'm uncomfortable with your implication here, Bobkat. Remember that you may build a house yourself, some day.
This is a much more agreeable suggestion. Throughout my whole life I've found that people are usually accomodating when dealt with honestly and straight-up.
|Posted by: Dell500 May 14 03, 11:53 AM GMT|
|I didn't think this would get so many replies lol. Well, I PC'ed some WBS, didn't come out right, looks really burnt. So going to try CC(Cracked COrn) today. Going to use the same tyvek filters too. But ya, a little off topic, but oh well, thanks for all the comments peeps. Peace|
|Posted by: psilli me May 15 03, 03:24 PM GMT|
I will trade Construction grade Tyvek for spore prints. Interested? PM me
|Posted by: Smerd May 15 03, 03:33 PM GMT|
|Someone over at myco supplied this link - http://www.intothewind.com/cgi-bin/detail.cgi?itemnum=402&sql=ks. It's a kite shop - pretty cheap, and it'd last quite a while.|