Culture source and cloning
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Either acquire a spore syringe or get started
from a fresh fruit body. Spores can be injected into the whole grain
recipe or the MMGG recipe. It /seems/ that spores work better in
the MMGG recipe, but more tests need to be made. After getting a strain
started, you can farm it for a long time with the procedure below.
Take a healthy mushroom for cloning
around the time that the veil breaks. You want one that is still
growing vigorously, not an abhort. Place it in the transfer chamber.
Avoid fruit with brown streaks on the stem. Use a sterilized Xacto
knife and tweezers to extract a clean chunk of stem to add to sterile water
in a mason jar.
This is done by holding the freshly picked
shroom near the cap, and using the sterile knife to cut a ring around the
stem about 2" from the bottom. Only cut a little way into the stem.
Use sterile tweezers to grab and peel the surface
layer back toward the base of the stem until it resembles a peeled banana.
Then cut the stem just above the peels.
Once you've removed the Al foil from the sterilized blade assembly and
mason jar, You have a sterile table to work on for cutting off the stub.
Then slice off the clean chunk and let it fall
into a mason jar (8 oz regular mouth) 2/3 full of sterilized water.
The jar is prepared by covering with the metal blade assembly and seal
from the blender and that is covered with Al foil and sterilized.
The transfer is done in the glove box. For more details see the "Blender
It turns out that a regular mouth mason jar has the
same size and thread as the plastic blender container part from an Osterizer
blender (probably other brands as well), so after dropping in the piece
of stem, remove the jar from the chamber, then screw on the base of the
blender and proceed to blend for about 3 or 4 seconds at the fastest
speed. Allow to stop, then repeat. Be sure to hold
the jar while blending. When done, remove the blender base,
put the jar (w/seal and blades) in the chamber, remove the blades
and seal, then immediately cover with a new baggie (in the transfer chamber).
Then draw syringes of the inoculant water by piercing the plastic
bag. This procedure has proved to be very effective.
For pictures of the details of the blender parts
and mason jar assembly go to the Blender usage
1) It is best to use a rubber band around the shroom water
jar/baggie to keep out contaminants.
2) If one cuts the peeled chunk of stem almost all the
way thru', then one can lift the blender parts just enough to stick the
stem thru and finish severing the sterile chunk from the rest of the stem
with the edge of the jar and the blender blade assy.
3) The shroom water has been stored for 1 week with no harm
and it's gone bad in 2 days. I have no idea how long it can remain
viable. It is MUCH better to make up the shroom water and use
it immediately. The growth rates are incredible when the shroom water
is fresh. One pint cakes (rice only) have been birthed 10 days after
inoculation. 14 to 21 days is more common.
4) The knife and tweezers are sterilized in Al
foil sheaths in the pressure cooker.
5) Eventually the cloned strain will lose stamina and
stop producing and growing vigorously. It is a good idea to take
a spore print and start a new culture every year or so (at least.)
See the spore print procedure.