Germination Problems

Nan's Nook : Archives : Jars : Germination Problems
  Subtopic Posts Updated Creator
Adding spores  5 02/01 05:02am Richard Warren
Additives??? (Honey?)  -    
Can't get spores to grow  19 01/18 07:14am kmd59
Help!!! 12 6/03 glasshoppa
Damn spores!  26 02/10 09:00pm Liberty_Caps
Incubation Problems  -    
Dishwasher residue preventing germination?  13 01/29 12:10am Martaxus
Single points of myc growth, re-inoc?  6 01/26 06:19pm ratdog

By Delekhan J. Delekhan (Delekhan) on Friday, January 18, 2002 - 09:59 pm:

Ok guys, I innoculated Tuesday afternoon, and it's now Friday afternoon. My jars have been incubating at ~85 degrees. Should I be seeing any signs of growth, because I'm not... Some of my jars are displaying a small amount of condensation, some look bone dry (depending on my sterilization method it seems), but none are displaying any whiteness.... Should I be seeing something by now, or should I be waiting patiently?


By Snoopy (Snoopy) on Friday, January 18, 2002 - 11:53 pm:

Keep waiting sometimes it can take a week to 10 days. Patience I'm sure something will happen soon. = )

By Dr. Cubensis (Shroomzilla) on Friday, January 18, 2002 - 11:59 pm:

What snoopy said :)

relax.... Make up some more jars if yer ansy...

By Delekhan J. Delekhan (Delekhan) on Saturday, January 19, 2002 - 12:32 am:

I'm not that ansy really, I just wasn't sure if I was supposed to see anything soon at all, or if it takes a little while for it to start going (enough to be visible i mean)... thanks guys


By Dr. Cubensis (Shroomzilla) on Saturday, January 19, 2002 - 01:13 am:

"sometimes it can take a week to 10 days."

Sometimes it's in three days or so, but more often than not ( in my friends experience ) it's at day four and beyond when you START to see growth...
The mycellia is working on colonizing your jars as we speak, when it gets to big to stay small, you will see it... Does that make sense???

By Chick (Schroomchick) on Friday, January 25, 2002 - 10:22 pm:

In my 1/4 pint jars, I saw white at 5 days, while in the 1/2 pint, it took about 7...hope that helps.

By kobayashi (Impalerzz) on Saturday, January 26, 2002 - 04:01 pm:

The mushroom teaches patience.

By Arevewe (Arevewe) on Monday, January 28, 2002 - 05:01 am:

This depends on the innoculation method and freshness of myc or spore. I saw solid colonization start within 48 hours with a fresh spore syringe (2 days from print). Also have seen 2 months to colonize because of excess water and crappy spores. You could also use a liquid tek and innoc with myc rather than spores. Since this is the myc already, the colonization will be much quicker.

By jim brown (Shrhobbyist) on Monday, January 28, 2002 - 05:49 am:

Chick, where did you find 1/4 pint jars? What brand are they?

By Chick (Schroomchick) on Tuesday, January 29, 2002 - 11:15 pm:

I got my 1/4 pint jars at Wal-Mart. This is the first year I have found them easy to find in my area. They are Ball jelly jars...

By Ricky (Retardricky) on Wednesday, January 30, 2002 - 07:57 pm:

Ok here we go. I mixed up brf and verm I used fish tank water cuz thats what I read works best. It has been 2 weeks today and only one jar is showing any growth. The one is about 25% colonized. No contams. Smells Ok at least through the holes in the jars. One jar has a black chunk in it but it hasn't changed size or shape so I think its just a big chunk of verm. Please let me know what you think.


By Bullwinkle (Bullwinkle) on Wednesday, January 30, 2002 - 08:07 pm:

I think the fishtank water is overkill. A cheap bottle of spring water is best cause its clean and pH is good.

You may have them too wet. This will cause bad aeration. Sounds like the holes arent plugged, that cuts down on air too. You could try cracking the lids a tad, and keep them at 80 deg if possible with heated water or such.

The only other possibility was bad spores by overheating the syring, supplier, etc.


By Jack Daniel (Jackdaniel) on Sunday, January 06, 2002 - 11:48 pm:

I'm having problems with germination and I'm pretty sure it's the syringes. The reason why is that the 12 - 16 jars I inoculate with one syringe will germinate fine, while the 12 - 16 jars I inoculate with a different syringe only germinate at 1 or 2 inoculation points, or not at all. Also, I have been doing this for a couple years now and never had a problem... I use to stretch one syringe across 30+ jars, but since I have been having this problem, I am using more inoculum per jar.

I order my syringes from Fanaticus and they have always been really reliable. Has anyone had any germination problems with their Mazatec or Hawaiian syringes lately?

Do the jars need light or have to be above a certain temperature to germinate? I have been inoculating them and then storing them in a dark room at a temperature of about 65-70F.

By jack cracker (Cptcracker) on Monday, January 07, 2002 - 12:47 am:

always better off to germinate at 82-84 if you can swing it. faster germination, and colonization. drop it back down to fruit...

By quote: (Quote) on Monday, January 07, 2002 - 02:12 am:

prolly lower spore count than you're used to.
you really should only do 10 jars per pf syringe.
streching it out will lead to many empty sectors.
but you can't blame pf if you don't follow directions.
pf tek specifies 1 cc per jar, .25 cc down each of 4 holes.
and he sells 10 cc syringes.
that equals 10 jars per.
and 65 is pretty cold for germination anyway.
more like 85 is where you want to be,
if only for the 3-5 days it takes to hatch the spores.

By Roc (Rochester) on Monday, January 07, 2002 - 04:18 am:

My beginners luck was good when I got started last spring... this winter it's been the shits until I faced the fact that those thais were in an area that averaged 85+ in the summer but that same area is only 70+ this winter, hell I just raised the temp with the fish heater/glass jar and boom, they are off and going. Temp is everything for incubation.

Temperature: Fruiting : Incubation : Lethal : Shroom Glossary