|By Professor Fustertingle (Fustertingle) on Tuesday, December 18, 2001 - 11:24 pm:|
I have a problem. I made a syringe the other day and when I looked at it today there is stringy bits of mycelluim sprouting about. Is the syringe garbage? Or might it still be useable?
|By jared (Jared112) on Tuesday, December 18, 2001 - 11:44 pm:|
If it has mycelium inside of it, and it's not contams. Hell If you use it right away It'll be even better than shooting a jar full of spores. It'll take off the spore germination time.
|By quote: (Quote) on Wednesday, December 19, 2001 - 12:20 am:|
it's prolly still useable.
apparently, your spores germinated.
|By An guy (Boomer) on Wednesday, December 19, 2001 - 12:26 am:|
I been wondering about this- what makes them *not* germinate in a regular syringe? Just lack of nutrients in distilled water?
|By Brettiejams (Brettiejams) on Wednesday, December 19, 2001 - 12:28 am:|
Hey Quote, generally, isn't it safe to say, that you need some food and heat for germination to occour?
|By quote: (Quote) on Wednesday, December 19, 2001 - 12:33 am:|
i've seen it happen in tapwater,
best to use distilled for syringes.
they don't really need food to germinate,
they're living off stored food until they find some.
heat + water can be enough.
By Kandiedan (Kandiedan) on Monday, April 30, 2001 - 04:57 am: Edit
I had some problems getting spores from spore prints to germinate. I'd appricate any comments about possible problems. I'll try and give as much detail as I can . . .
1) It all started about two month ago. I got spore syringe from PF and got started following PF tek . . . everyhting worked grate
4-12-2001 - I made 5 spore prints from my first flush. I stored these in one of my drying containters tupper ware with damp rid.
4-17-2001 - I make a spore syringe from one of the spore prints. I make the syringe by pressure cooking a syringe with water. When it cooled I scraped of some spores and mixed them in shot glass. I used this syringe to inocc. 4 1/2 pint bell jars. I placed these jars in my closet.(these jars are now fully colonized).
4-22-2001 - I used spores from three of the spore prints to inocc. 8 more jars (total so 2 jars with one on the syringes and 3 jars with the other two syringes). I wanted to try using a fish tank heater to keep the jars warm so I placed four of the jars in a bowl of water under my desk and places the other four right next to them as control.
I GOT VERY POOR GENMINATION
at this point (4-30-2001) only two of the eight jars have germinated at all and those only in one spot.
I used the exact same method to generate the spore prints and spore syringes. There is no contam problem just no germination.
I let everthing (syringes, water, shot glass, jars) cool to room temp before using.
Any idea what could have happen.
Could I ahve killed the spores from exposure to light or altoides or oxidization of tin . . . (I stored the prints in an old altoids box)
Do spores need to be in the dark to germenate . . .
Any ideas would be usefull, as I will try and repeate my experiments and figure out what happend . . .
By Quote (Quote) on Monday, April 30, 2001 - 05:49 am: Edit
soak spores in water 24 hours before using.
|By Professor Fustertingle (Fustertingle) on Thursday, February 07, 2002 - 10:23 pm:|
Hello and good day. Everyday when I come here, I learn something new and I try to put it to use. Lately I have been trying to make my own syringes. But in my haste I have not made one single successful syringe. All three I've tried to make have been contaminated. I have never tried a glove box. Is this my problem? According to some of the syringe teks in the archives a glove box is not a required piece of equipment. I've tried the oven tek without success. Is it possible that the prints I am using are contaminated or do I need to make a glovebox? I got these prints from what I consider to be a reliable source. Maybe I just need someone to tell me that, "Syringes are easy to make, you just need practice and patience."
|By quote: (Quote) on Friday, February 08, 2002 - 01:54 am:|
i would at least make a cardboard glovebox,
it really is a distinct improvement over open air or oven door techniques.
take a halp pint jar and drill a hole in the rim of the lid & then stuff a wad of polyfil
as much as you can wedge in.
trim it down afterwards to size.
sterilize the jar, let it cool,
then take your print on the jar's bottom.
working inside your glovebox, clean the cap, but not the gills, with a q-tip dipped in tincture of iodine. then place the cap in the jar and tighten the lid.
let it sit until you can see the print under the cap, then in the glovebox, remove the cap and reseal the jar.
when you get ready to make syringes, rinse the print off first with a syringeful of sterile water, just stick the needle thru the polyfil plug, squirt the print, then tilt the jar and draw up the fluid. discard it, it will carry 90% of any potential contams.
then to get a spore syringe, simply repeat the process, inject sterile water, you might need to draw it up and re-squirt it a few times to get plenty of spores, but remember if you can see tiny dark dots of spores, that's plenty, you don't need dark syringes.
|By I feel so weird (Phishgrower) on Friday, February 08, 2002 - 06:23 am:|
Here's another idea that uses supplies you probably already have laying around. Not sure if you read or not, but a while back I made a glovebox and immediately proceeded to accidently blow it up. So I decided it was time to try something else.
Take an extra sterilite container (55L) and clean it out. Then spray it down with a lot of lysol and put the lid on. This will keep the lysol inside and kill all germs.
Next you want to have 4 pre-sterilized syringes, your print, cotton balls, paper towels, a shot glass, and any dull knife. Take rubbing alcohol and sterilize the knife blade, syringes and shot glass and put them all into the sterilite container. Then take rubbing alcohol on paper towel and wipe your hands and fore arms. When you are ready to begin work turn the sterilite on its side and just work around the inside. (I'm assuming you've read on glovebox procedure for print to syringe methods). The dull knife is to scrape the spores off the print. It works much better than using the tip of the needle in my opinion.
There's no need to keep it completely air tight. I make 4 syringes out of one print and they are all very dark. I've done this about 10 times so far and I've had 100% success.