|Strain Isolation Tek||3||
|Inoculation - Inoculation Loops||?||
|Kimax glass petri dishes||4||Fishy1|
|By J. Tay (Recombinant) on Wednesday, February 06, 2002 - 06:35 pm:|
After the top of the agar (In a 1/2 pt. jar) has been fully colonized by mycelium, is it at that point safe to open the jar to remove excess moisture, etc. like a regular cake?
Or, does the myc actually colonize the whole thing? (I'm doubtful of this one.)
And, after the terminal point in myc colonization of the agar, is it okay to remove from incubation and store in a refrigerator?
[coming soon: Agar TEK for enormous dummies w/o scales to measure out 300g of potatoes]
|By Maliki vision seaker (Maliki) on Wednesday, February 06, 2002 - 07:05 pm:|
I have done exactly that to my agar cultures a few times. This was how i made my dextrose culture liquids. I waited untill the mycelium has coverd the surface of a tall half pint . Then with a sterile exacto I scraped the surface bare except for the very center and reclosed the jars. All of the above being done in my clean box. Put back into incubation chamber let the culture grow out again and repeated around 5 times. Now the culture sits completly top surface covered and down the sides of the jar as well in my refrigireator. Its kinda blue but ok otherwise. Just started new culture yesterday, today it has fresh growth.