Pinning - help?!

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Pinning!!!  -    

By JJ (Jjf) on Friday, February 08, 2002 - 07:08 am:

Hi everyone - I'm new to this board :D I'm hoping I can get a little help in regards to gettin these magical friends of ours to fruit. I'm using the PES Hawaiian strain from sporeworks and did a multispore innoculation. I managed to get 5 jars fully colonized and about a fortnight ago I birthed 2 of them. I made 2 casings with the other 3. The smaller casing has a top and bottom layer of verm and the bigger casing just has a top layer. I did the vooodoo tek on the bigger cake, and the double ended casing tek on the smaller one. Temps vary from 70 - 80. I tried coldshocking the two cakes and the smaller casing yesterday and I'm waiting to see the results of that. There are a couple of tiny little white spindly things sticking out from the cakes but these haven't grown for a week. Everything's in a rubbermaid bin with perlite humidification. I'm guessing the humidity is close to 100%. I fan it 3 times a day. Any ideas or help welcome :D
O and I just thought I'd add - they were exposed to light the whole way through colonization (both the jars used for cakes and the jars used for the casings)

Small Casing:
Small Casing

Big Casing:
Big Casing

Small Cake:
Small Cake

Big Cake:
Big Cake

By quote: (Quote) on Friday, February 08, 2002 - 02:16 pm:

not much else you can do,
but wait patiently.
isn't a 'fortnight' like 40 days ?
surely you don't mean they've been birthed that long ?

By JJ (Jjf) on Friday, February 08, 2002 - 02:42 pm:

Heh. Noo it's 2 weeks.. (comes from Middle English fourtenight, alteration of fourtene night, fourteen nights - according to Thanks quote! Umm also is it really true that 25% of all multispore innoculations just don't fruit at all? How unlucky would if by some miracle they were all non-fruiters :~(

By jim brown (Shrhobbyist) on Friday, February 08, 2002 - 04:49 pm:

You say you innoculated the same cakes with different spores? I have never tried that before because I have heard it is a bad idea. What the theory is is that some types of spores will grow together and some types won't. So it would seem all of yours would or all would not produce depending on the compatiability of the spores. And if they did produce it would be smaller than that of a single spore innoculation. By the way, where did you get that 25% figure?

By quote: (Quote) on Friday, February 08, 2002 - 05:06 pm:

no, since we inoculate at 4 different points, there's almost always a fruitable substrain on each cake.
but if you only inoculated from one point, there'd still be more than one substrain produced since hundreds of spores are germinated at each point.
so cakes almost always yield something, although bad luck genetically can give you pretty low yields.

By JJ (Jjf) on Friday, February 08, 2002 - 05:28 pm:

Ahh I understand now.. thanks for clarifying that quote! I did innoculate at 4 different points..
Just on that topic actually.. I've only been able to ever make 1 syringe that worked (out of about 10). The rest were all green mould failures. The 1 time I got it right I started working inside a little cardboard glove box but it got too cramped so I threw it off and finished the job - worked fine.. Haven't managed to repeat that since.
Do you think I should build a proper glovebox? Umm also - do I need a fan with a filter thing or can I just use a box with two holes for gloves, and spray it down with some bleach solution prior to making the syringe?

jim brown: I think you misunderstand.. a multispore innoculation is simply one done with a spore print - from what I understand, if you grow out a strain on agar then you know what you're working with.. this way you don't.

By jim brown (Shrhobbyist) on Friday, February 08, 2002 - 05:38 pm:

Okay, now I understand. I kind of wondered why you would have used different spores on the same cake. I thought that's what you meant by multi-spore.

By JJ (Jjf) on Friday, February 08, 2002 - 05:46 pm:

Ya.. sorry if you already know this but I'll just clarify for you anyway - a spore print contains millions of spores. Hence when you do a use a spore print you're doing a multispore innoculation - thousands of spores in suspension in the water. More than one of these spores germinates in the substrate. Hence multispore innoculation.

By Brettiejams (Brettiejams) on Friday, February 08, 2002 - 05:53 pm:

Multi spore innoculation = innoculating with more than one spore. Not different strains mind you.

And the spores may or may not come from a print.

There are ways to make syringes up that skip the print part... and when you innoculate with one of these syringes, it is still a multi spore innoculation.

Make sense?

By jim brown (Shrhobbyist) on Friday, February 08, 2002 - 07:57 pm:

I got it now, thanks. multi-spore innoculation sure does beat the old single spore innoculation, huh?

By Brettiejams (Brettiejams) on Friday, February 08, 2002 - 10:04 pm:

lol.... sure does!

By JJ (Jjf) on Sunday, February 10, 2002 - 06:52 am:

The day after my last post my big cake got pins Here's a couple of pics - I'm wondering if this first pin is an abort - it's quite dark in colour.


Some pins
Some pins

Some more pins (see the tiny little two below :))
Some more pins

My small casing is yet to pin - there's condensation on the mycelium, I'm wondering if that's a bad thing.. should I put it in a terrarium with lower humidity?

Closeup of small casing
Closeup of small casing

My big casing wasn't doing much so I took it out of its container and made it into a kind of flatcake thing:

Flatcake round thing
Flatcake round thing

By plinkerdink420 (Plinkerdink420) on Sunday, February 10, 2002 - 07:26 am:

i don't think it should be that wet... the condensation is a bad thing i think.. that first pic looks like an abhort to me, though

By quote: (Quote) on Sunday, February 10, 2002 - 03:46 pm:

does look a bit over-wet.
but that's not an abort, yet, it may go either way.

By onediadem (Onediadem) on Sunday, February 10, 2002 - 05:16 pm:

Very nice pics. I wish my camera could take pics like that.
Good job. Hope all your pins grow up.

By plinkerdink420 (Plinkerdink420) on Sunday, February 10, 2002 - 05:24 pm:

yeah, sometimes i think a pin is gonna abhort and i look at it a few hours later and it's doubled in size... these things really baffle me sometimes... i'm sure that will come with time though.... sorry for any misleading information

By JJ (Jjf) on Sunday, February 10, 2002 - 05:53 pm:

Cool thanks guys :) That one wet abort lookin thing is still growing so I guess we'll see. There are now 15 pins on this cake.. it's crazy :D

O and any other comments on the casing perchance?

onediadem: its macro capabilities aren't great.. closeups like that are not the sharpest. it's a canon digital ixus 300 (about $500 US?) - though I'd recommend the Nikon Coolpix 5000 if you've got the money :D

By plinkerdink420 (Plinkerdink420) on Sunday, February 10, 2002 - 06:07 pm:

i would replace that paper plate with aluminum foil and let it dry out a little bit so it can start pinning... it could take several days, though...

By JJ (Jjf) on Sunday, February 10, 2002 - 06:16 pm:

That's not a paper plate - it's ceramic. Also umm that's not the casing I meant heh - that's the flatcake thing. The casing's the one with the closeup. It's in a plastic tray thing:

On that note.. I couldn't figure out how to show that previously uploaded image (from another thread) in this post.. anyone?

By quote: (Quote) on Sunday, February 10, 2002 - 10:10 pm:

you can't show a previously uploaded image directly, you have to either link to it as you did, or re-load it from your hard drive.

By JJ (Jjf) on Monday, February 11, 2002 - 07:00 am:

Ok thanks quote. Also - does anyone reckon that those shrooms will be harvestable come Saturday morning? (Friday night for you people from da USA). Reason I ask is - in a dream we were all going up for a hike in the rainforest on saturday and I thought trippin there would be kinda cool :D

By quote: (Quote) on Monday, February 11, 2002 - 02:41 pm:

if it's warm enough, they should have no problem being ready in 5 more days. might not quite yet be full sized, but prolly close.

By JJ (Jjf) on Monday, February 11, 2002 - 03:31 pm:

Thank again quote
I have yet another question (heh sorry).. the dosage calculator on the shroomery seems somewhat strange for fresh weight consumption. Is 17.6grams fresh really a level 3 trip? That would dry out to around 1.8grams... *confused* What fresh weight would you recommend for somewhere between a level 2 and 3 trip for a bunch of firstimers out in the rainforest?

By quote: (Quote) on Monday, February 11, 2002 - 06:36 pm:

fresh is often more potent than dried, i'd say the shroomery's dose estimate is not far off the mark. stay with less than 30 fresh grams and you should be safe, 20 would prolly be a bit more 'comfortable' but 30 will fry you hard,
assuming normal tolerance and potency levels.

Troubleshooting Casings : Dosage