|Agar Plates (pic)||-|
|By J. Tay (Recombinant) on Thursday, February 07, 2002 - 06:21 am:|
I happened upon success with this operation guided by my intuition, after failed attempts w/Quote's "simple" agar tek, (what do YOU consider "large" potatoes?) and frustration trying to imagine what 300g of potatoes would be with out a super-expensive balance. Let's rock.
1.) Grate 1 potato to occupy 2 cups distilled water.
2.) Boil for 30 minutes, strain, add distilled water to 1L volume.
3.) Add 2 tbsp agar, 1 tbsp dextrose, 1/2 tsp yeast.
4.) simmer while stirring (but not beating) for 10 minutes.
5.) Pour into 1/2 pints (wound up with enough for four)
6.) PC w/foil covers, lids band up @ 15psi for 30 minutes.
7.) Cooled gradually so as not to create that sucking in dirty air by cooling too fast effect described by Quo. (But didn't wait for the PC to cool on its own either)
8.) Knocked up in my new ghetto-fabulous glovebox.
9.) Placed in incubator @ ~80-85 degrees.
10.) Waited for 1 week, yield = success.
Hope this can help anyone else in my position that wants to rock liquid culture like a mo'
|By onediadem (Onediadem) on Thursday, February 07, 2002 - 12:36 pm:|
Is this like the karo tek? Or does the agar solidify like on a petri dish? sorta confused here. Perhaps I need more info for a dummie.
|By quote: (Quote) on Thursday, February 07, 2002 - 01:42 pm:|
agar solidifies 'like on a petri dish'.
|By onediadem (Onediadem) on Thursday, February 07, 2002 - 03:38 pm:|
Ah, I get the post now. The liquid culture threw me. "rock liquid culture like a mo'"
I sees, lol.
More on Agar (btm thread)
|By Kenny Keller (Kennieyk) on Saturday, February 09, 2002 - 06:11 pm:|
I wanna do some petri dishes.,and it seems i need agar. So where can i get this. Is there something else i could use in plase of agar?
|By quote: (Quote) on Saturday, February 09, 2002 - 06:13 pm:|
i found some at my local health food store...
also you can order online, for example at fungi.com
|By Kenny Keller (Kennieyk) on Saturday, February 09, 2002 - 07:15 pm:|
i can give that a try. what did you ask for or where did you look in there? Whats it used for ?
I just dont wannna look stupid asking for something i dont know what its used for . thanks
|By C K (Phrozendata) on Saturday, February 09, 2002 - 07:31 pm:|
You need agar and nutrients
|By quote: (Quote) on Saturday, February 09, 2002 - 07:46 pm:|
just ask for agar, it's seaweed gel, someone there should know if they carry it.
as for nutrients in your agar, there are several suitable formulas here in the archives.
|Posted by: czen Nov 12 02, 12:07 PM GMT|
| I'm getting ready to try messing with agar. I bought some agar/agar at an asian market and I'm not sure I got the right stuff. I was expecting a powder or something like that. The stuff I got is sheets of strands about like linguine. I've ground it up in a blender which was about like trying to grind styrofoam. Is this the right stuff or should I use gelatin instead?
Have a good one,
|Posted by: Nanook Nov 12 02, 01:20 PM GMT|
| You got the right stuff. It comes in various forms: powder, sticks, sheets.
Weigh it out and dissolve in boiling water, it will work fine
|Posted by: czen Nov 12 02, 02:02 PM GMT|
| Cool. Now on to growing something that will likely glow in the dark.
|Posted by: OneDiaDem Nov 12 02, 08:24 PM GMT|
|Growing glow? I find that night vision goggles help tremendously. Jump into the agar, dont be shy. It really isnt all that intimidating if you have a glovebox and a pc.|
|Posted by: czen Nov 13 02, 06:20 PM GMT|
| The agar is going well so far. The potato dextrose agar gelled up like it is supposed to instead of remaining a slimy potato broth. I placed it in the glove box and inoculated with a tiny drop from a dirty syringe that I want to see if I can resurrect.
I used wide mouth half pint jars which are a pain since I don't have clear lids. Are presterilized disposable plastic petri dishes suitable? Will they withstand pressure cooking or must I pour in a glove box? I guess they'd be fine for peroxidated agar.
|Posted by: Nanook Nov 13 02, 09:08 PM GMT|
|Try ebay. Presterilized plastic dishes come in packs of 20 usually. Spray the plastic sealed bag of dishes down with lysol and put in the box with hot pressure cooked agar in a decanter. Open the bag, pour and stack plates. Leave everything sit in the box until cooled|
|Posted by: czen Nov 13 02, 09:45 PM GMT|
|I'll give that a shot. Thanks again for the help.|
|Posted by: OneDiaDem Nov 13 02, 11:15 PM GMT|
|Keep in mind, no spores on peroxidated agar. Only mushroom tissue and mycellium.|