|By Martaxus (Martaxus) on Monday, February 04, 2002 - 06:59 am:|
Oy! I've just inoculated 12 jars of ricewater to grow inoculum. With this or the Honey Tek, is 1/3-1/2 cc spores enough for a good inoculation?
Is there a minimum amount recommended?
|By plinkerdink420 (Plinkerdink420) on Monday, February 04, 2002 - 11:52 pm:|
i have used 1/4 cc before and it worked fine... i think there just needs to be enough spores to germinate and mate...
|By Hatcher (Hatcher) on Tuesday, February 05, 2002 - 01:38 am:|
Failin' miserably at liquid culture teks..
|By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 02:00 am:|
What's the problem Hatcher?
|By Hatcher (Hatcher) on Tuesday, February 05, 2002 - 02:44 am:|
God knows..I've only tried a couple jars, (1/2pt. jellies), but thought I'd get it right the first time. The first jar, 100ml/tps. karo, is now 30 days old. It's finally got a blob of mycelium floatin' around in there, would probably do a syringe, but nothin' like the cornucopia of mycelium I sorta expected. I've tried lowerin' the time in the pc. The spores are my own, but have been juicin' jars with 'em..(First time I've had slow/no colonization from my own syringes too, but I'm sure the solution saw enough spores, surely..)
|By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 02:55 am:|
It sounds to me like either incubation temps are too low, or you had slow germination due to low spore count.
Maybee try a P.F. syringe next time?
|By Hatcher (Hatcher) on Tuesday, February 05, 2002 - 03:07 am:|
That'd be a good control, but still..
I'm gonna give that first jar a prime spot in my new styrofoam/old milk bottle/aquarium heater deal, see what she does. In a mobile home on a hill now, never bothered w/such in an apt. But I keep the bedroom pretty warm w/ an oil-filled radiator type heater.
|By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 03:15 am:|
Cool, did you check out the one I just built?
Pickle-jar heater(incubator version)
|By Hatcher (Hatcher) on Tuesday, February 05, 2002 - 04:02 am:|
Cool Brettie..my father has alot of old bottles, wasn't hard to find an old square milk bottle that the glass tube of the heater fit close w/, masking tape. I was more worried about having to replace the water than humidity in the cooler..well, never worried 'bout that, ain't come up...
I've sat the jars away from the heated water like you did, but the heat isn't penetrating, w/ the thermometer reading 86F 4 inches away, the nearest jar 3. What about shootin' for 93-95F on the mercury? Thermal death is at 104-107 or somethin', right? The glass insulates, then the dead-air space in the verm it's self..
Think I'll turn up the heat.
|By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 04:28 am:|
The bigger the jar (Greater the water volume), the more stable the heat will be.
I wouldn't go much over 90 on the heat,
Even though, as you say, thermal death occurs at 104, if you get over like 94 the myc starts using more water than it normaly would, and the cakes go into a sort of "stressed" growing.
Best to keep it under 94
|By Hatcher (Hatcher) on Tuesday, February 05, 2002 - 06:26 am:|
Kinda what I was wonderin', at what temp would max conditions occur, and beyond that would start to detrimental. Thing is, can't measre the temp inside a jar. And the micro-climates our individual set-ups create..Trial 'n' error, observation, the man tools of the mycophile!..
|By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 08:33 am:|
Usually like 2 degrees hotter in the jar, from what I understand, never stuck a thermometer in there though.