Quick invitro question

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By Arthur Oliveri (Zappa_Crappa) on Thursday, January 24, 2002 - 05:32 am:

Been doing the invitro tek since christmas time - pins started a week or so ago, now little blobs forming.

My question: Should I take out the plain vermiculite layer to allow more room for growing, or just keep it in there.

Things look a bit cramped as they are.

Any feedback - I couldn't find this touched upon in the archives.
Thanks yanks.

By Cragith Kilbonith (Kilborn) on Thursday, January 24, 2002 - 06:40 am:

I have never grown using this tek before but I remember a thread while back someone saying that you should remove the verm.

By Imok Urok2 (Imok) on Thursday, January 24, 2002 - 08:29 am:

Hey Arthur,
Check out Chronic007's current thread
Making my life easier.......Quote? Eatyualive?
on his invitro thread in growing.
In a nutshell, he transfers his 1/2 pint jars to
1 quart jars with spacers between the cake and
the lid and the lid on plastic/metal egg crate to
allow holes in the lids to let the cake breath.
The jar provides a mini-environment with space
for the mushies to grow into.
Hope this helps :)

By nuecrew (Nue) on Thursday, January 24, 2002 - 09:15 am:

I tried to understand this and looked for it in the thread. Could you try one more time to describe his technique? About 1/2 pints to 1 pint, egg crates, spacers.

By An guy (Boomer) on Thursday, January 24, 2002 - 09:25 am:

it seemed to me, he grows his 1/2pts, births them into qts, with the cake on a spacer so it's up off the lid so it doesn't block the holes in the lid, which are basically the holes you punch to innoculate, only now they're being used for air transfer, and then he's using expanded metal for shelves.

By quote: (Quote) on Thursday, January 24, 2002 - 10:30 am:

i leave the verm. usually intact for 1st flush, but remove it after that.

By Dr. Cubensis (Shroomzilla) on Thursday, January 24, 2002 - 05:59 pm:

Sounds like you are not transferring to quarts/pints???

"About 1/2 pints to 1 pint"

You are doing in-vitro in the same jars that you are colonizing in? or transferring to pints?

Be real specific when ya post and you will get more specific help :)

By quote: (Quote) on Thursday, January 24, 2002 - 06:04 pm:

he's using the customary pre-chronic tek definition of invitro, meaning in the same jar it started out.
you should probably assume that to be the case in most instances.

By Chronic007 (Chronic007) on Thursday, January 24, 2002 - 06:46 pm:

Arthur Oliveri: I leave the verm. on for the first flush same as quote when doing quo's classic invitro tek.

Newcrew: click on the link in Imok's post and make sure to start reading through the archived parts of the thread at the top of the page first, it describes every aspect of the "chronic tek" perfectly.

Dr.Cubensis: Hey bro how’s it going? Ya, quo's right, "invitro" most always means quos 1/2 pint jar through entire process tek. Great tek!. I did most of my learning on it before doing a bit of my own experimenting. It's awesome for low pro operations.

Take care and be safe

By Dr. Cubensis (Shroomzilla) on Thursday, January 24, 2002 - 07:11 pm:

Yea Meestor Chronic!

As I said previously, had never thought about or considered in-vitro. Thought that it was soley for the " furtive" grower... When I saw you were rebirthing into quarts, well, that put a WHOLE new spin on things....

My Apologies Arthur!

As for my friend.... Oh yea. He has been DROWNED
in success using quarts in-vitro...

Quick update for you on progress...

Dunked Rye grains, taking a LONG time...
First ( in-vitro ) pinning was witnessed starting
only three days ago. Will wiegh and publish findings here at Nan's Nook when they all finish thier friuting cycle.

Seed/dung casings dunked, then cut into cubes and stashed invitro... Was a bust. pinning from every angle of " cake " was rampant... Tiny shrooms, not worth the effort.

Full wide mouth pints cut in half... BEST flushes.
two cut in half made for four quarts. Getting cakes outta jars was tricky, but the flush was extraordinary in comparison to the tall pints.

Tall pints... Amazingly enough, Most tall pints
gave my friend ONE LARGE mushie and a lot of small ones. but when weighed dry on a trpl Beem, those large mushies were about an 8th by themselves per quart...
* whew! *

Now we're playing with power!!!

By ggg (Ggg) on Thursday, January 24, 2002 - 08:48 pm:

My pal fruits many invitro cakes and he says that when he loads the jar he uses the double ended tek. He puts about 1/4" of verm in his 1/2 pints before he loads his substrate. This results in a slightly smaller cake providing a little more room for fruit. It also improves colonization time. This bottom verm remains with the cake. His layer of verm on top of the substrate (the contam barrier) is removed at the first dunk. It is replaced by a clean 1/4" layer for fruiting.
The effect is a 1/4" double ended casing invitro and my pal is VERY satisfied with his results.

By Arthur Oliveri (Zappa_Crappa) on Friday, January 25, 2002 - 02:39 am:

Thanks quote + chronic - I will leave the vermiculite in for the first flush.

This is my first time so i dont want to go too crazy and birth to a full quart jar. I want to go with the PF+quote classic invitro.

thanks - really doesnt look like enough room in there though, i believe in you guys though!


By Imok Urok2 (Imok) on Friday, January 25, 2002 - 08:53 am:

I think Quote's got some pics in mycoporn
(in the archives) showing some clasic invitro.
Very impressive stuff :)