Lots of Invitro Discussion Here

Nan's Nook : Archives : Jars : Growing invitro? : Lots of Invitro Discussion Here
  Subtopic Posts Updated Creator
Black head  5 12/24 06:13pm Mr. Tambourine Man
Flipping Invitro Jars  5 12/31 09:10pm  
Invitro ==|)'s  16 12/03 09:56pm Timothy Leary
Invitro Question, Drop Temps?      
White fuzzies on shrooms  12 12/13 12:46am Mr. Tambourine Man
Quick invitro question  13 01/25 08:53am Arthur Oliveri
Birthing 1/2 pints to quarts for invitro fruiting  13 12/13 04:46am Mr. Tambourine Man
Loosening jar lids for invitro fruiting?  3 12/13 02:39am Mr. Tambourine Man

By The Silly Scybe Scribe (Toadstool_God) on Tuesday, December 25, 2001 - 09:41 pm:

Lo all! Once again happy hollidays. Anyway Ive got a couple of questions. First...once my cakes are completly colonized and have formed pins I am going to pre flush dunk them as they are PFC and Ive heard they benifit from the dunk. Im using the Quopy Neglect tek and I was curious. Would it be a good a idea to put a small layer of vermiculite on the bottom of the jar before I put em in as well as on top? Would this be kinda like invitro double casing? Next question..are the cakes more prone to "puddling" i.e water pooling at the bottom of the jar after a dunking? If so is it neccasary to empty this out? Thanks all.

By Brettiejams (Brettiejams) on Wednesday, December 26, 2001 - 07:13 pm:

If you pat your cake dry after the dunk, I see no reason to fear any kind of "pooling" taking place.

Invitro fruiting's biggest obstacle, in my thinking, is the lack of good air exchange.

That is why the cakes are more prone to contams when fruiting in-vitro.

I wouldn't add any verm post colonization to an in-vitro cake.... the stale air coupled with the uncolonized verm is just asking for trouble, if you ask me.

And there is very little moisture loss in an in-vitro cake.... other than what is lost to the shroom during development... so a verm casing is not as imperative as a cake fruited in the traditional terrarium.

I haven't done a ton of in-vitro work.... so these are educated guesses, really.

I'm sure Quote will straighted me out if any of the info I have offered is contrary to his own experience.

I feel pretty good that my theories are sound, though.

By quote: (Quote) on Wednesday, December 26, 2001 - 07:48 pm:

i think it's arguable whether cakes are more susceptable to contams while invitro. true, there's not much air exchange ordinarily, but there's nothing stopping a grower from opening his jars daily to exchange air, dry the glass, etc. and being in a jar actually protects it from many contams which are airborne, or on the grower.
you definitly want to remove pooling water, etc.
i'd pass on the verm. in the bottom, it really isn't needed and adds unnecessary risk.

By The Silly Scybe Scribe (Toadstool_God) on Thursday, December 27, 2001 - 07:05 am:

Quopy or anyone. Could I use the cooked brown rice substrate tek and still do invitro fruiting? If so how much water to rice? Still quarter cup?

By Eatyualive (Eatyualive) on Thursday, December 27, 2001 - 09:41 am:

yes because i have about 12 jars growing invitro as we speak. it seems the brown rice cakes want to pin much faster than normal.

By quote: (Quote) on Thursday, December 27, 2001 - 03:12 pm:

never tried it, so i dunno....

By quote: (Quote) on Thursday, December 27, 2001 - 11:34 pm:

there are plenty of invitro success stories,
and lots of people fail at all of the other teks as well..
it's usually the grower's fault, too.
not the tek's.

By mr. mista (Mistabud) on Friday, December 28, 2001 - 12:04 am:

Your right quote...Here is another success story. I'm not quite sure how you could screw this tek up, but oh well.
DCP_0034 (Medium)

By mr. mista (Mistabud) on Friday, December 28, 2001 - 12:16 am:

Also, check out this little cluster of shrooms. You can't see them all, but there are at least 22 in that little cluster.
Do you think they'll all grow? or abort?
DCP_0040 (Medium

By mr. mista (Mistabud) on Friday, December 28, 2001 - 03:18 am:

Yeah, thats what I thought.

By the way...Is it ok to have all that condesation in my jars?

Should I wipe out the jars?


By quote: (Quote) on Friday, December 28, 2001 - 03:55 am:

some condensation is normal, but it wouldn't hurt to dry them once a week or so. or whenever you open them up, etc.

By mr. mista (Mistabud) on Friday, December 28, 2001 - 04:28 am:

Cool, thanks

By Stewie (Stew) on Wednesday, January 23, 2002 - 09:21 pm:

Ok, Im growing invitro and I dunked before my first flush and the results were good. I then dunked after the first flush when they didn't really need it. now all my cakes are growing tiny little shrooms that are over saturated with water.Many of them are dieing or just stop growing.

I was thinking that maybe i should clean my cakes and let them sit under a fan over night. Does anyone think this would be beneficial or should I just let them be?

By quote: (Quote) on Wednesday, January 23, 2002 - 11:16 pm:

i wouldn't put them directly under a fan to fast dry it, you'll kill the outer surface mycellia. better to slowly let it lose the excess water, just don't give it any more for awhile. and do as nan suggested...

By quote: (Quote) on Thursday, January 24, 2002 - 04:23 pm:

i understood they are invitro, but fast drying is bad, it kills. slow and easy does it.

By Arthur Oliveri (Zappa_Crappa) on Tuesday, February 05, 2002 - 04:47 am:

I am doing invitro
it has been 5 1/2 weeks. 8 jars. they all have tiny fruits but they just stopped growing a week or so ago. One mushroom got big but looks like top is tearing. is when the tip turns black a sign to birth? I am disappointed. There aer about 10 tiny little runts on each of the cakes. They are all starting to turn black at the little tip. Are they stopped growing? Is this the degenerate - or is it on my end - or should i just birth and get 1 good trip out of the bunch? disappointed.
thanks yanks.

By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 05:14 am:

Well, you can take the cakes out of the jar anytime you want and then put them back in if that is what you mean by "birthing" them.

Black = rot. gotta pick those ones pronto.

The one that the top is tearing you can pick as well.

Are you growing the P.F. race, if so, the spores clould have been of the degenerated race, but your shrooms could all abhort if you don't have enough water in your substrate also.

Could be that they could use a good Dunk

By Drstrange (Drstrange) on Thursday, February 07, 2002 - 03:20 pm:

About to start 1st time, Quote Neglect Tek. One last question (unless another arises ): Any benefit or loss from making 1/2 pint cakes in pint jars and fill the bottom with a few extra inches of verm. My thought was that when if starts to pin I could carefully remove cake (like at harvest) and loose the extra verm, leaving some more space for a bit easier growth and harvest. Sort of a mini Chronic version without the risers. Any advise on the pros and cons of this would be appreciated! Thanks

By Chronic007 (Chronic007) on Thursday, February 07, 2002 - 08:19 pm:

Hmmmm.....Well this really is quotes field and I'm sure he'll be around soon to give you a more definitive answer but I'll see what I can do until then. The very first tek I tried was quo's neglect/invitro tek and one thing is for sure, it works great as is. If your only motivation for the extra verm is extra space for fruiting and you donít want to put the minimal effort in for a chronic tek terrarium than I think your idea is the only path left toward that goal. Keep in mind however that after the first flush the cakes shrink considerably and the have plenty of room to flush in half pints on their second flush. All in all Iím sure your idea will work fine. Good luck and be safe.

By quote: (Quote) on Thursday, February 07, 2002 - 08:40 pm:

bottom or top,
split the difference,
matters little.
just be sure that all your inoculant doesn't drain into the bottom, where there's no food.