Can't get P. F. spores to grow

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By kmd59 (Kev) on Wednesday, January 16, 2002 - 02:02 am:

frist off i am know to Growing shrooms but i've been reading for 2 month's on this site and www.fananticus and have read the tek at least a dozen times and followed it but i might be doing something wrong. i cleaned my kitchen and sprayed it down with 20% bleach mixed brf then steamed 10 tall 1/2 pint for 60 min. and shot them with hawaiian and mazatec from p.f. (i've read he is the best to order from) 2 c.c. each in the oven and put them in a styrofoam incubator they were still warm to the touch that been four weeks now
and nothing so i figure they were to warm because i read anything over 95 degrees kills the spores so i ordered more syringes from p.f. another hawaiian, mazatec and this time a p.f. olso got a p.c. at wal-mart tried the honey tek to stretch the syringes i've tried it 3 time in the last week and
still nothing so i'm down to my last 4 c.c. of each
and don't know what to do now the only thing i can thik of is i live in the antrax scare and maybe there nuked
thanks kev

p.s. any help would be appeciated
agian thank's

By Liberty_Caps (Liberty_Caps) on Wednesday, January 16, 2002 - 02:35 am:

you got my kind of problem the devil is curseing you
but PF's spores are almost 100% garented to grow
you waste alot of money tho buyeing them

By Dr. Cubensis (Shroomzilla) on Wednesday, January 16, 2002 - 02:41 am:

You could have gotten bleach in your brf mix. That would spell disaster for sure.

You waited till they COMPLETELY cooled before you innoculated, right? the heat from steaming the jars can easily kill your spores. Even if the jars are cool on the outside, they can be VERY hot inside.

Doubt it was the mail, but it could have been.

3 times on a honey tek with nothing? three separate times or three jars?

Anyway, you are having an extreme run of bad luck, or you are doing something horribly wrong.

I'm just floating thru and know nothing, so don't take my word as gospel, but someone who does should be along shortly...

By Fanaticus (Fanaticus) on Wednesday, January 16, 2002 - 02:45 am:

If your jars were "still warm to the touch"
after you inoculated them, I am sure the
spores died (to hot in the jar).

the honey tek is not a sure fire thing
for beginners.

What is with the "oven" thing?
whatever it is, you don't need to do it.

I don't think you followed the directions
at all.


By Dr. Cubensis (Shroomzilla) on Wednesday, January 16, 2002 - 03:12 am:


Pf has a fantastic record.

My friend followed the pf tek in
fact with his first growing experience.

If you feel you followed the PF tek to the letter.
Then talk to pf Privately and try to resolve it.

You really need to try again IMHO and this time, even if you already have, follow the tek to the LETTER.

Good luck

By kmd59 (Kev) on Wednesday, January 16, 2002 - 03:14 am:

P.f. that what i though on the frist for the oven thing is i read a glove box should be used but you could heat your oven to 200 for 20 min.
then pull out the lower rack inoculate them there to help stop contaminations.

dr. cubensis i sprayed the kitchen took a shower and then started the tek and 3 jars 3 times
thanks guy's

By kmd59 (Kev) on Wednesday, January 16, 2002 - 03:17 am:

p.f. I did follow it printed it out and had it in font of me the whole time

By An guy (Boomer) on Wednesday, January 16, 2002 - 03:20 am:

let your jars cool over night in the thing you cook them in.**

give those 3 sets of jars a few more days- it's too soon to see anything if you just shot them up this last week. The first one's might be showing something. takes anywhere from a few days to upwards of a week sometimes. I'm guessing you didn't get growth in the honey, which means you shot straight spores, and the honey water might have been too hot also. Shooting spores in honey water into a jar of substrate increases contam chance. Shooting mycelium in honey water into a jar of substrate isn't as chancey.

Stay away from the honey tek for now. Have some success with a straight pf tek first, get some confidence, and then try it. Don't try to stretch those syringes. If you get some fruits, learn to print, you can mess with syringes all you want, and same with honey/karo tek.

Try strictly straight pf for a few jars, and let them cool before you shoot them. mostly sounds like it's impatience on your part which is screwing things up. That's understandable, but it's really worthwhile to have some patience.

Good luck


By kmd59 (Kev) on Wednesday, January 16, 2002 - 03:26 am:

anyguy all the honey jar where cooled over night and i will try agian with what i have left

By plinkerdink420 (Plinkerdink420) on Wednesday, January 16, 2002 - 02:18 pm:

i use the oven tek very often... and i like it... i use the top rack in the uppermost position, though, pull it out all the way, and close the door up to it... haven't had a single contamination since using it..

By Kman (Kman) on Wednesday, January 16, 2002 - 05:17 pm:

ya I use the oven tek too, but I heat the sone bitch to like 500 to kill anything inside then I put it on low and just use the oven door, it makes a nice big working area. I got 3 bad jars my very first time I grew and didn't use the oven tek, since using the oven o% contamination although I do have some humidity issues to figure out still.

By I feel so weird (Phishgrower) on Wednesday, January 16, 2002 - 05:42 pm:

Make sure you know how to use your pressure cooker. That's what killed me when I first started. If you have a weight on top, it has to rattle up and down. Start the time of the pressure cooking when it starts to do this. It should bob 1-6 times per minute. good luck. peace

By Herman Manderchuck (Youenjoymyself) on Wednesday, January 16, 2002 - 06:20 pm:

Stick to the basics...Try the PF tek a couple times before jumping into Honey and all that jazz...And I Wouldnt recommend incubating in the oven...If your jars are warm to the touch during incubation thats probably too hot.Cool temps can only slow growth.HEAT can mean Murder!! Stay up and try again! Good Luck.

By Maliki vision seaker (Maliki) on Wednesday, January 16, 2002 - 07:04 pm:

Just my 2 cents here to kev59 . Go read a litle more go check out the PDA agar recipie . Its simple to make. With the agar your garentied to be able to spot growth. And use just a tiny drop and you can grow out more inoculum than you could posibly need in around 2 weeks time. If there is one thing that i have learned it it is things take time that are worth doing . But the times are shortened with the more advanced you get at least to a point . Then the mushes take there time too even though were worried abought them not comming up. Use what you have and maximise your chances for succes. Glove box and ALL

By plinkerdink420 (Plinkerdink420) on Wednesday, January 16, 2002 - 11:42 pm:

oven incubation is bad... makes for dry substrate

By Doc Thirst (Doc_Thirst) on Thursday, January 17, 2002 - 06:14 pm:

Looked through the archives but didn't find it, can someone drop a link to the PDA agar recipie when they have a moment?

By Maliki vision seaker (Maliki) on Thursday, January 17, 2002 - 06:26 pm:

PDA (Potato Dextrose Yeast Agar): Wash 250 grams of unpeeled potatoes and slice them 1/8 inch thick.
Wash these several times in cool tap water until the water is clear. Drain the slices in a collander and rinse once
with distilled water. Cook the potato slices in distilled water until tender. Strain the cooking liquids through a
flannel cloth or several layers of cheesecloth and collect the liquid in a flask. Rinse the boiled potatoes several
times with distilled water, add these rinse waters to the liquid in the flask, and discard the potatoes. Add enough
distilled water to the flask to make one liter. Bring the liquid to a boil and add 15 grams of agar, 10 grams of
dextrose, and 1.5 grams of yeast extract. The agar must be added slowly and carefully to prevent boiling over.
While the liquid is hot, pour it into petri dishes or other culture containers. Each should be filled half way.

PDY (Potato Dextrose Yeast broth): PDY broth is made in exactly the same manner except the agar is
omitted. Mason jars are filled half way with the hot or cool liquid.

MEA (Malt Extract Agar): To one liter of gently boiling water (distilled) add 20 grams of malt extract, 20
grams of agar (slowly, carefully to prevent boiling over), 100 mg of potassium phosphate dibasic (K2HPO4), and
100 mg of calcium carbonate. While still hot pour the liquid into the culture dishes.

The most difficult item to get for me was the dextrose powder. I wound up finding a single pharmicy out off around literley 25 or so. The day i picked it up I found out i could just go by an ounce or two at a brew store for wine and bear. Agar healthfood stores. Potatos we hope no explanation needed on where to get them, other than white older is better potatos . Kinda soft but not mush. But any will do.

By Doc Thirst (Doc_Thirst) on Thursday, January 17, 2002 - 08:07 pm:

Holly cow! What a receipe! Gonna have to give that one a try. Don't know about the rest of you, but I often have to ask myself (while reading this board) how the hell did someone come up with that! Thanks for the info Maliki.

By Maliki vision seaker (Maliki) on Friday, January 18, 2002 - 07:14 am:

Actualy agar was discoverd by accident in china i think . Its just seaweed jelly a cook left out and it jelled at room temperatures. The potatos are for your starches and protiens as most things need them to grow. And the dextrose is just a refined sugar to suport rapid growth. The agar is just the sterile media that holds the nutients in suspension. Like gelatine, but it jells at a much warmer temperature.

Temperature: Fruiting : Incubation : Lethal : Shroom Glossary