Syringe Sterilization Tek Needed

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By Kevin Smith (Canshroom) on Sunday, January 13, 2002 - 08:28 am:

I'm following the spore syringe tek in the archives, but the water in my syringes has been boiling off during the boiling stage, decreasing the amount of water that I can inject into the jar. Is an hour too long for them to boil/steam? Or could my syringes be at fault? If anyone knows this tek, please help.

By Pinhead (Pinhead) on Sunday, January 13, 2002 - 08:59 am:

your PC is leaking, it seems that when your PC ing syringes, if the presure cooker is losing steam alot, because of high boiling volocity the water in the syringe must be boiling too .

I had success bringing my pc to presure and backing the temp down on the stove to a point were the steam doesnt come out very much, still a little to insure maitain temp but less water escapes.

ALSO very important, wrap you syringes in a clean washcloth and then in tinfoil a few times, and use the cover from the needle to to maintain needle clean till last minute.

I have had success with this method but every now and then it still happens,

I tried a piece of rubber on the end, and tapped the plunger of the syringe after pulling a vaccumm of air but it didnt work. i only did it once and the plunger came out

also I dont know if your PC ing spores or Myciliam or just water but I was under the impression that this killed those things, if anyone knows weither it does or not, let me know.

I use syringes to inject water into my cakes after ever flush and before the first . I also put a little dextrose in the water seems to give it a little kick. I am working on a iv drip idea to feed dextrose water to the cake all the time. i will post if this works, waiting for iv bags
I don't like dunking my cakes cause it takes so long. I like to put the water in the center of the cake where it remains for a long time.

By ggg (Ggg) on Sunday, January 13, 2002 - 10:03 am:

you could keep a small pot of distilled water boiling off to the side of the sterilization pot.
This boiling water can be drawn up into the syringe to replace any distilled water that had boiled off during sterilization.

By Brad (Raze) on Sunday, January 13, 2002 - 05:09 pm:

Um, are you letting the PC cool fully before opening it? If you just pull the release pressure valve the water is gonna all boil out on you. When the pressure's gone, the water is still hot enough to boil and w/o the pressure, it will
And yes, heat kills spores. Thus the reason we sterilze our jars (to kill mold spores and such)

By awalter44 (Farmerinthedell) on Sunday, January 13, 2002 - 05:20 pm:

What if someone just used rubbing alcohol to clean the syringe? Would it kill the spores?

By plinkerdink420 (Plinkerdink420) on Sunday, January 13, 2002 - 05:24 pm:

if there is any alcohol residue left in the syringe, it will probably kill your spores

By An guy (Boomer) on Sunday, January 13, 2002 - 06:56 pm:

might try just steaming them empty, and drawing them up from a jar of spore water. Worked for me a couple times so far. Tried it because mine kept boiling themselves empty too.

By Kevin Smith (Canshroom) on Sunday, January 13, 2002 - 09:29 pm:

Thanks for all the info...not sure if it changes things, but I don't have a pressure cooker, so I steam them. I think you're right though...the water inside the syringes was probably boiling off. What if you just sucked boiling water into the syringe and left it there to cool? Does it really matter what contams may be on the outside? Or would exposure for that short a time not do the trick?

By An guy (Boomer) on Sunday, January 13, 2002 - 11:28 pm:

they say to suck up a syringeful of boiling water, and then expel it, then suck up another one, and let that one cool and use that second water.

I didn't do it that way just because I printed into a jar, added some sterilized water, and then in a box sucked up several syringes which I had steamed, and taken directly to the box still in the little deal I had steamed them in. Seemed cleaner that way to me. YMMV

By An guy (Boomer) on Sunday, January 13, 2002 - 11:31 pm:

oops- then I found out it was much easier to print directly into a jar of sterilized water, mix it up, and syringe from that.

Printing into a dry jar kinda sucked for me- spores stuck to the glass, and I had to manually hand mix them up into the water, and although it came out all right, I hated all that stirring action above an open print jar, moving my arm around.

By Kevin Smith (Canshroom) on Monday, January 14, 2002 - 12:43 am:

Boomer...what tek did you use to print directly into the sterile water? Sounds like an easier way to do it.

By An guy (Boomer) on Monday, January 14, 2002 - 03:18 am:


Steamed a jar of distilled with a lid on kinda loose just like a regular 1/2pt of br. Covered that in aluminum foil.

put it in the box, after cooling in the pot. Kept it covered on the way to the box.

In the box, took it all off.

Had a cap ready- had slipped a ziploc over it when it was still on the caked, picked it in the bag, took it to the box.

had two popsicle sticks cut like waterbed supports, and notched at the ends where they laid on the edge of the jar top. put them together like you do waterbed supports (I had steamed them along with the jar of water and had them wrapped in foil) on top of the open jar, reached into the ziploc, pinched the stem off the cap, then, still in the bag, I lowered the cap down onto the sticks. Gently took the bag off, and covered the cap, sticks, and jar with with sterile foil, domed so it wouldn't touch the cap, left it all in the box for a day. A fresh ziploc that hadnt' been opened yet would have done nicely too, and been watchable... The whole time the fans ran, it's a pos. pressure box.

Steamed a lid in foil that I had drilled off-center, so I could get a needle down into the edge of the bottom to get every last drop, more or less, took it into the box, took it out of the foil, took off the foil/stick/cap arrangement on the jar of water, saw a nice, thick, black/purple print on top of the water, screwed on the steamed lid and tightened it. the box had been sprayed and wiped with alchohol before operations commenced, and again when I did the cap/lid transfer, as it had been sitting there for over a day. Even with the fans running, I thought it was prudent.

Took the jar out of the box, shook it up, got a nice jar of spore water. when I made syringes, some stuck to the glass, but were pretty easy to squirt off it, lots easier than the one's I printed straight to the dry jar were.

Won't say how many syringes I got, but I had filled that jar mostly up.....they all were lightly purple. Just shot some jars last night, so we'll see how it goes.

I think though, other folks told me about this in a basic way, I'm sure you could easily adapt it to a stove top or oven door....

By An guy (Boomer) on Monday, January 14, 2002 - 03:20 am:

Sorry- When I steamed the jar of distilled, I steamed it with the foil on- that makes a kind of air shield when I carry it to the box. Don't keep it sterile, but does help.