|By Liberty_Caps (Liberty_Caps) on Friday, January 11, 2002 - 08:37 pm:|
ok I have a quiestion I just made 3 syringes of the hawwian race useing the oven tek with a print the size of a 1/2 pint jar.. so now I have 3 really dark syringes and 4 days ago I injected 4 1/2 pint jars... well my previous FROZEN pf syringe is germinationg before my fresh hawwian spores....
so i was curious can spores just not germinate????? I used 2/1/2 cc's on each jar but in 8 diffrent places maby i injected to many spores for them not to germinate... could this be the case I'll give them a few more days but does anyone else ever have problems germinating spores???
thanx for your time.
|By Dr. Cubensis (Shroomzilla) on Friday, January 11, 2002 - 08:39 pm:|
They just need more time, IMHO...
Odds are your spores will germinate.
Keep the jars warm and BE PATIENT!!
|By Joe Mamma (Madscientist) on Saturday, January 12, 2002 - 01:58 am:|
I took a cap and had it drop its spores on some honey water. I incubated it along side all sorts of mycelia water. It took 1-2 weeks for everything else to grow out. It took the spore jar two months. It took 6 weeks just to start germinating. Once it started it took off fine. Something held the germination off.
|By Liberty_Caps (Liberty_Caps) on Monday, January 14, 2002 - 09:53 pm:|
ok I sent a dollar to Ralph to get an ecudor print but today in the mail I got my print but its not an ecudor print it says this
P. Tasmain 11/11/01 by kali (wood strain)
what the hell is a wood strain?????????
|By Dr. Cubensis (Shroomzilla) on Monday, January 14, 2002 - 11:37 pm:|
My friend will send you some prints if you like.
Think they are equador, but might be romero....
Pretty confused on the species... Stopped keeping track and decided they are all the same basically...
Will get some prints from my friend and send tommorrow if you get to me in time tonight.
Just let me know where, consider them there.
Started out with ralphsters spore ring to, but remember, you don't get to pick, it's a spore ring, you get what he gives you and be thankful you are getting spores for a dollar in the first place.
|By Dr. Cubensis (Shroomzilla) on Monday, January 14, 2002 - 11:39 pm:|
you have spores..
Sorry, mind is on other things today
Forget everything i just said!
|By Liberty_Caps (Liberty_Caps) on Tuesday, January 15, 2002 - 05:19 am:|
but I seem to be haveing extream trouble on getting my home made spore syringes to germinite
WHAT THE FUCK IS GOING ON??? why dont these basterds germinate???? like I said above thoese jars have no mycelium growing yet......
I do all my work as steril as possible it takes like an hour to make some syringes and everytime I do they suck grrrr
|By Hatcher (Hatcher) on Tuesday, January 15, 2002 - 06:39 am:|
I sent Ralph a dollar too, the thread had fishy1 mailin' 'em to ralph, TC & GT. Just hussled down to the mailbox, score TC! Man, too cool. Somethin' tells me the world would be a better place if it had more mycophiles in it!
Liberty...dunno. I got five Eq spore syringes I just made up on the living room floor in front of me, pulled 'em up in here, expect them to be fine. I've only ever done it one way..sporulating caps dropped into 1/2pt. jars, inoculum made in those, one hole in the lid to draw the stuff out, a couple small pieces of glass to get the spores up off the bottom. Yet to have a real problem. Sounds like ya just ain't gettin' any spores in your syringe...
Get to try the ole print and shotglass soon if I'm gonna add a fifth race to my collection! If you're listening, thanks guys!
|By Hatcher (Hatcher) on Tuesday, January 15, 2002 - 06:47 am:|
But Liberty, you said the syringes were DARK, didn't ya? Dark how?..
|By Liberty_Caps (Liberty_Caps) on Tuesday, January 15, 2002 - 09:47 pm:|
how black?? really black, sometimes and sometimes dark purple..
because I'll only make like 3 syringes off a 1/2 pint sized print
so thiere pleanty of spores in the syringe but they never geminate or contam the jars just sit thiere and after they havent germinated for a week they start to get weird looking not like a contam but like ageing/rotting BRF bcuase the jars are always in the incubator at 86* for weeks
why would these spores not germinate?????
Ive only came acrosse a b+ print that worked, and Pf syringes I dont understan why they wont grow?
|By quote: (Quote) on Tuesday, January 15, 2002 - 11:33 pm:|
there's no good reason to make syringes so dark, you might notice that pf's aren't.
as for your problem, you need to tell us in exact detail your entire process for getting from sporulating cap to loaded syringe, perhaps we can spot the error.
|By Liberty_Caps (Liberty_Caps) on Wednesday, January 16, 2002 - 12:57 am:|
ok thanx quope im going to try to do that right now...
ok so first I bought some spores frome PF then I injected thoese spores into my jars made 8 hawwian jars 24 days later ,, I THEN birthed thoes jars the first flush usaully has tons of skiny shrooms...
but for printing I thought I'd wait till the 2 nd flush (better for printing I gusse)
so once the seconed flush came buy I had some really nice big shrooms so yes!!!!!! I was going to make some prints so I waited probly 7 hours after the veil broke then before,,,,, I opened the rubbermaid container to get the caps to print,
I washed my hands in achole with a cotton ball, then washed my hands really good went back into my room sprayed my room down with lysole let it settle.. then proceded to open my terrium pick the mushroom and quickly and swiftly cut the cap off never touching the gills!!!! so then I proceded to take that cap and put it in a STERLIZED half pint jar the put the lid on loosely, then I let them print for 34 hours then I tighted the lid down... took them downstaries to heat up the oven and use the oven tek ,,,,I boiled 4 syringes for 6 mins then sucked boiling water into them 3 times then shot it into the sink/not back into the water then sucked up 4 more syringes let them cool on my window cell,
then once they were ready I took the caps out took a (sterlized )key thingy scraped the spores on the jar bottom so they would mix in with the water. then took my 4 cooled syringes of water shot it all into the jar through a little hole on the lid that was taped (while) printing,,, then shoke the jar a little took my syringes sucked up the spores shot them out one time then sucked the spores back up into my syringes put the caps on the syringes back on taped them shut then injected 4 jars the next day now 8 days later nothing/no growth WHY WHY WHY????
hope thats everything
|By Fanaticus (Fanaticus) on Wednesday, January 16, 2002 - 02:53 am:|
If it has been 8 days and no germination, that is a bit overdue. You should see something by now.
There is another possibility. The spore solutions might be contaminated. If there is bacteria in there, you will see no germination, but you can detect a powerful rotten garbage kind of smell if you slightly loosen the jar lid
and take a wiff.
|By plinkerdink420 (Plinkerdink420) on Wednesday, January 16, 2002 - 02:15 pm:|
is it possible that a slight amount of the lysol or rubbing alcohol got into the jars that you were printing in and killed the spores?
|By Hatcher (Hatcher) on Friday, January 18, 2002 - 07:25 am:|
If you're not growing ANYthing, including contams, other than maybe not giving them time to rehydrate(think they would even in substrate really tho..), ya almost gotta be killin' 'em some way, or they've mutated sterile some way...
|By J. Tay (Recombinant) on Friday, January 18, 2002 - 08:12 am:|
I feel you Liberty. I've made prints and syringes by the book and its totally hit or miss.
"Maybe new verm fucked up my substrate and I need to modify the ratio to accomodate the new anomaly?" No, b/c 2/4 spots have growth.
"Maybe its the winter and the jars aren't warm enough?" Put 'em in a cooler wrapped w/heat blanket. "Went over 90, maybe heat killed spores?" Nope, this proved to be false also.
So what's the fucking deal, eh? I can't figure it out. Fuck it. JUST GIVE UP! ;)
|By Liberty_Caps (Liberty_Caps) on Friday, January 18, 2002 - 07:03 pm:|
I am giveing up, I'll just waste 20$ on spores every few weeks
I don't understand why it happened yes thoes jars are still thier, thiers nothing growing, no contams or myc, but last night I opened a jar and put a fresh piece of mycelium in the substrate to see if that will grow, now I'll wait and see
|By quote: (Quote) on Friday, January 18, 2002 - 08:15 pm:|
alot of folks run into problems when they begin to make their own syringes, even the pros screw up every now and then.
i see nothing obviously wrong with the procedure used, but the devil is in the details,
as they say.
for most hobby-scale growers,
it's prolly best to buy ready-made syringes which are guaranteed, and which can then be extended into enough liquid mycellial solution do do hundreds of jars per syringe.
that should give anyone enough to cheaply play with.
then you can master your own technique without the pressure.
|By Liberty_Caps (Liberty_Caps) on Friday, January 18, 2002 - 09:00 pm:|
well hey that sounds good, I gusse if I make some karo jars I gusse could have alot more syringes to play around with.. I'll make a jar tonight or tommrow but before I do. do I just fill a half pint jar full of clean steril water add some(HOW MUCH?????? ) karo syrupe? or honey?? then boil for how long???? an hour???? or 30 mins????
ps. Ive never done it before so Im on my way to the archives to study. mabey I shouldent of asked thoes quiestions but oh well
|By plinkerdink420 (Plinkerdink420) on Saturday, January 19, 2002 - 12:28 am:|
Nan's karo tek
Temperature: Fruiting : Incubation : Lethal : Shroom Glossary
|By Hatcher (Hatcher) on Saturday, January 19, 2002 - 10:11 am:|
Dunno guys, advising lib to forego making his own syringes from spores? This is something he needs to do...
Don't give up hoss, you can do this. Hell, my first veil broke only a year ago this Feb. 17th. I'm only now attempting liquid culture, and the results haven't been what I had hoped, almost expected. Still tring tho, duh! But I can still knock-up all the jars I want w/spores...now how dd you say you printed your caps caps??
|By Maliki vision seaker (Maliki) on Saturday, January 19, 2002 - 02:54 pm:|
Hay Liberty , the one method most seem to forget and just skip is the agar tek . It works wonders you can scan for germination right there in front of you , nice and clear no obstruction. Then simply transfer the mycaeium from the agar to liquid tek and make your syringes that way. It just seems like a process that can be observed much closer and once you make a good jar of inoculum you just have to suck up a new syringe instead of start the whole process again. 24 days is all it took for me to see the fruits of my labors. I could have sworn it was longer though.
|By quote: (Quote) on Saturday, January 19, 2002 - 03:21 pm:|
i recommend mastering liquid mycellial techniques before printing/syringe making, not instead of.
although there is no requirement that one make syringes at all, many people just buy commercially made syringes and that's ok,
we're not trying to turn everyone into master mycologists, we're just trying to help them grow some shrooms.
but consider the progression logically.
liquid mycellia should be the very first technique we teach a newbie.
you can whip up a jar of mycellial water as soon as you rec'v your very first syringe, before you ever grow a crop to even get a print.
if one uses polyfil plugs in the lid, as i do, you can make mycellial water syringes without a glovebox, pressure cooker, or any special equipment other than polyfil and a half-pint jar.
1 cc of spore solution from the commercially bought & fully guaranteed syringe can easily make enough mycellial water to fill 20 syringes,
and he can do that 10x before he needs another.
that's roughly 2000 jars from one commercial syringe, without ever taking a print.
that way, any newbie can afford to play around at his own pace, gradually acquiring more skill and equipment with full confidence that he's not going to have to spend more $$$.
how many times have we seen newbies crying out because they've only got 1 syringe and they used it to make 8 jars and they all contam'd, or fruited poorly, or messed up in some way ?
or as liberty cap said "I am giveing up, I'll just waste 20$ on spores every few weeks?
too many times, and such problems are easily avoided by learning liquid culture first.
|By Hatcher (Hatcher) on Saturday, January 19, 2002 - 07:46 pm:|
Point taken quo and the advice, as always, excellent. But, never had a real problem w/spores, and liquid culture is turning into a stumbling block. Haven't lost confidence tho--never say die!
|By Liberty_Caps (Liberty_Caps) on Saturday, January 19, 2002 - 11:20 pm:|
the thing I cant really understand is why my first 2 prints were good/sterial and they actually grew faster then my PF myc but now I have been doomed.
so I'm going to try printing again with some fresh cakes, in a fresh terrium, and then maby these B+ prints will work this time around
PF or any other vendor must be makeing a shit load of money because you could make like a gallon jug of spore sloution with 1 big dark print. if you wanted to make them as clear as PF does
|By Hatcher (Hatcher) on Sunday, January 20, 2002 - 12:25 pm:|
PF's syringes have never failed me, no matter what they looked like, and a damn fair price imo. Shouldn't really discuss such matters here, also, imo.
Are you printing directly into the jar you're going to make your inoculum in L.C.? If not, try that. I always keep the KISS principle in mind.