|By Dr. Cubesis III (Newbieshroomer) on Friday, December 07, 2001 - 06:05 pm: The Nook|
NewbieShroomers EZ FoolProof innoculation Tek.
Ok, I wrote this for a guy who's subject line was " FAILURE " at the shroomery.
I am sure that I will be attacked there for offering it up to the general public
I always am when I contribute ( must be my breath ) So I am including it here, Hopefully I will only get constructive critisism as opposed to viscious attacks.
Maybe a newbie can make some use of it if it makes it into the archives, it works for me, every time.....
If you are like me, you do not wanna spend hours and hours cleaning
just to innoculate your damn jars. I have been using this EZ tek
for a while now and every jar takes off clean. It takes no time
at all to set up. Oh, nothing in here is original ( as if! ), but
it's the way I DO these things.
You will need:
A viable Syringe
PURE cotton pads
Disinfectant wipes ( WITHOUT BLEACH )
Latex Goves ( un-powdered )
Something to sit on while you innoculate.
And that's it!!!
Assuming you are using a tek that involves foil, do not bother with any tape, any " Magic discs " or silmilar crap. You don't need tape or disks, unless you are EXTREMELY unsterile or live in an EXTREMELY unsterile enviroment.
First, Before pressure cooking your jars, put the foil on the prepared jar.
Make sure it's around down around an inch below the top of the lid. Form fit it to the lid and rub your finger over the nail holes. you should see visible depressions in the foil so now you know where the nail holes are WITHOUT taking off the foil. These will be your innoculation points and innoculation is done THRU the foil.
Pressure cook for about an hour at 15 psi. don't forget to put a towel or a rack down. Don't wanna dry your jars out. Let cool overnight. Do not touch the PC again after you turn the oven off. You probably wanna get to it and cool your jars quickly, but why risk your spore juice? Why risk all the work putting your jars together?? So JUST WAIT. If you have a job, or school, it's perfect, PC in the evening, you are innoculating the next evening.
Now the Fun Part! My Flavorite at least! Turn the oven on to 200 degrees, then, start gathering your materials. You need to make an alcohol lamp, so simple take a few cotton balls, set them lightly in the shot glass, fill the shot glass halfway with the alcohol.
Spray some lysol on the counter, all over the kitchen, put your crap down, open the oven ( leave it on low ) and go take a shower, be sure to use soap and brush your teeth. Gargling won't hurt either.
You are out of the shower now, waves of soft warm heat are coming from the oven,
you are ready. Take the jars out of the pc and set on oven door about 3 to four inches back, if you have a lot, make two rows.
On the left or right top corner of the oven, set the alcohol lamp, a wipe, your syringe, your lighter, thats all you need. We are going to be FANATICAL about out needle sterilization. This will stop contams from going jar to jar thru the needle. This is the part it is said when it is easiest to contam your jars.
Time to put on the gloves, rub your arms down with rubbing alcohol, spray the gloves with lysol after they are on, rub the can down with lysol soaked gloves, then spray the gloves again. Wait till gloves dry ( less than a minute ) Light the lamp, and here we go!
Between innoculting EACH hole ( NOT each jar, but each hole ) we will be flaming the needle, NOT red hot, but you will hear a satisfying sizzle and then wiping on the disinfectant pad, hold the pad over the needle until it cools, and right before innoculation. this lessens the chance that contams will land on the needle. If you have the spore juice to spare, squirt a little out between flamings, You will be piercing the foil with the needle, DO NOT take the foil off at any point, there is no need. After however many holes you choose to innoculate are done, simple TWIST the foil an inch to the right. The holes in the foil no longer match up with the holes in the jar. Do this after each jar to lessen the chance something might fall in. Then store in a dark warm place,
Forget about them for a month ( LOL! ) then come back and check out your success!
Try this with EACH jar and you should not have any contam probs, and even if you do, you are not likely to lose a whole batch. This tek looks long, but it is oh-so-simple after you have it down... I hope this tek helps someone, it sure has helped me! :0) Questions? Email Me at [email protected]
If things get any worse, I'll have to ask you to stop helping me.
Too many freaks, not enough circuses.
|By jim brown (Shrhobbyist) on Friday, December 07, 2001 - 07:03 pm: The Nook|
That is a bit excessive for PF-tek innoculations. And that last sentence was a bit of an understatement. And I'm not sure if flaming the needle between every shot and waiting for it to cool is so EZ. More like tedious...and redundant. Maybe this is a good tek for Pigpen from the Peanuts cartoon but a bit much for the rest of us. I mean no offense but it doesn't seem right to avoid spending an hour cleaning your area (which you don't need to do either for PF innocs) by spending even more time showering and flaming and waiting for the needle to cool at least 48 times.
|By Dr. Cubesis III (Newbieshroomer) on Friday, December 07, 2001 - 07:13 pm: The Nook|
Read the part at the top about WHY I Wrote this jim. the guy had contams. He was new, it was his first time. subject, failure. At the shroomery, there are TONS of new people every day.
This is a FOOLPROOF tek.
For the NEW NEWBIES
Built to work every time.
In case you haven't noticed, NEW NEWBIES constantly have contam problems. This is for them, as they get better they can cut steps out or create thier own, so in other words, this tek is not directed toward you in any way
Anyway, Thanks for the critisism...
|By Nan (Nanook) on Friday, December 07, 2001 - 07:14 pm: The Nook|
It's a good tek though. I use a similar one for Glovebox work when I am working with open Liquid Cultures. I agree it's a little overboard for Shooting PF Jars, but I will find a place to load it in the archives when the time comes. I mean for me, I shoot jars in a Clean Room because I am filling Syringes there... so why not. And I do not flame the needle between every contact, my technique is pretty fluid, I flame before I go into the source culture with the needle, but after that I only wipe with an alcohol patch if the needle pulls up gum.
Not bad all in all, good effort.
|By Lichen (Lichen) on Saturday, December 08, 2001 - 12:18 am: The Nook|
technically, jars should be 'steamed' for an hour; pressure-cooked for 30 minutes at 12lbs.
Innoculation is easy and you should be able to do it anywhere. Just lift the tape, insert sterile needle through the holes, and use one cc per jar. If the needle comes into contact with anything besides the contents of the sterile jar, flame it in an alcohol flame, or wipe with an alcohol cottonball. It also may be a good idea to flame or wipe the needle periodically, just to hedge the bet...but a whole lotta pedantic steps shouldn't be necessary
|By UnkyHerb (Notapplicable) on Friday, December 07, 2001 - 07:47 pm: The Nook|
Heh.. I'm so lazy.. but no contams. How do I innoculate?
Steam jars for 30 minutes, let them sit for a few hours on a table near a window until they cool.
Turn the oven on 400 (my oven BLOWS, so to get any heat out, it has to be this high).. pull up a chair, put jars on the floor next to me. Put on oven rack take off foil, wipe the syringe with rubbing alchohol, shoot the 4 holes, put tinfoil back on it and stick em in a brown paper bag. Then the next jar, same thing, I wipe my needle inbetween jars.
Fastest way i've found to do it
Wanna know the secret? Stay still. Only thing that should move is your arm
Oh, I don't take a shower before either.. I've even done jars after comming home from a weekend camptrip without a shower.. imagine how dirty I was?
I do wash my hands though.. then dry them on a filthy towel just outta spite :o
I also live in a dirty apartment, no where near sterile.. but fairly clean to the eye ;)
Just don't do something stupid, and your jars will be fine :o
|By Imok Urok2 (Imok) on Sunday, December 09, 2001 - 01:04 am: The Nook|
jim brown, reading Dr. C's post, I think he covered your concern:
"Between innoculting EACH hole ( NOT each jar, but each hole ) we will be flaming the needle, NOT red hot, but you will hear a satisfying sizzle and then wiping on the disinfectant pad, hold the pad over the needle until it cools, and right before innoculation. this lessens the chance that contams will land on the needle.
It doesn't hurt a newbie to be too carefull, especially where contams
are concerned (and it was written with a problem with contams in mind).
The writeup may be too long and redundant for some, but others
may need exectly that (like me sometimes)