|By An guy (Boomer) on Sunday, December 23, 2001 - 06:00 am: The Nook|
I know I saw it somewhere some time ago, but can't find it now and have to confirm-
Spores sticking to the inside of the syringe barrel-
How do you fix this?
Am I recalling correctly that you suck in some sterile air for a bubble, and use that to shake the syringe up?
|By goodNyou (Goodnyou) on Sunday, December 23, 2001 - 06:21 am: The Nook|
i think that would work fine..
pump a little out and just suck pure air into the syringe..suck the air from in front of the stove if you want.
i think 1/2 cc of air would suffice and then shaking the piss out of it would be easy...
or youcould just leave it..
if you have spores stuck to the sides that is visable.. you got like 10 billion others that you cant see(never mind the ones you can)
persoally i wouldnt bother doing anything, but i just feel talkative
|By onediadem (Onediadem) on Sunday, December 23, 2001 - 07:52 am: The Nook|
not that i ahve anything experiance wise to donate, but how about drawing air with the needle stuck in a wad of polyfiber? just an idea
|By Nan (Nanook) on Sunday, December 23, 2001 - 07:38 pm: The Nook|
If it were sterilized polyfil it would work great. Sterile cotton here is OK. Sterile cotton balls can be used for needle wipes too.
On the spores sticking to plastic... Use a suspension agent or wetting agent in your water.
See: Nan's Print/Syringe Tek
|By Hatcher (Hatcher) on Sunday, December 23, 2001 - 08:51 pm: The Nook|
I sterilize my syringes filled w/10cc of water and the airspace. You can shoot the sterile water down the sink leaving just enough to preserve the sterile air,or into your innoculum jar to effectively make more innoculum, given there's plenty of spores in your jar. Just now getting into my second year, I could only guess how long a man could get away with that!
Also, agree w/everything good gotta say!
Merry X-mas gentlemen!
|By An guy (Boomer) on Monday, December 24, 2001 - 05:59 am: The Nook|
Thanks for your input folks. I'll be mulling it all over, synthesizing things.
What I got to thinking was that maybe if you had a glovebox or positive pressure box and an alchohol lamp, maybe you could, inside the box, have your lamp going, being very careful of things in general, take a syringe, squirt some out over the flame, and then with the tip of the needle either right in the flame or right in front of it, pull the plunger back, suck air from the flame.
|By quote: (Quote) on Monday, December 24, 2001 - 10:34 pm: The Nook|
i guess one could pc a jar full of air, polyfil-plug in the lid, and draw up 100% sterile air inside it.
|By Hatcher (Hatcher) on Monday, December 24, 2001 - 11:07 pm: The Nook|
I'm w/Good--pull it from the stove. That's where I do my sterile work, good ole' electric range. I make my own prints and syringes with the thing. No glovebox, no HEPA filter, and no problems. Yet, anyway. Keepin' it simple.
|By ion ewe (Ion) on Monday, December 31, 2001 - 11:07 am: The Nook|
If you sterilize a jar of air, just make sure you don't open the PC for a full day to let those jars cool. If they're warm when you pull them out, they'll just suck in a bunch of dirty air. And if you try to thwart this by just sealing them tight after sterilizing, you'll end up with more serious problems... like negative pressure...
|By quote: (Quote) on Monday, December 31, 2001 - 02:50 pm: The Nook|
hence, the polyfil plug in the lid.
|By Anno Nymer (Anno) on Monday, December 31, 2001 - 03:53 pm: The Nook|
Suck app. 1cc of sterile air into the syringe by placing the tip of the needle into the flame of a lighter or an alcohol lamp and slowly pulling the plunger back.
|By ion ewe (Ion) on Wednesday, January 02, 2002 - 03:47 am: The Nook|
I know, Quo. That's why I mentioned the sucking action. If it sucks dirty air, the polyfill will not help much as it is not a super snazzy micron filter. Polyfill will keep out stuff from normal slow exchanges, but not strong suction.
The thwarting would be attempting this procedure without a polyfill lid or some other breathable cover... like those vaccuum cleaner bags I was talking about.
|By Hatcher (Hatcher) on Wednesday, January 02, 2002 - 07:34 am: The Nook|
Like the ida Anno! I strive for simplicity. Like a machine, the less parts there are, the less that can go wrong. I don't try to keep it simple ONLY because I'm lazy...=)
|By quote: (Quote) on Wednesday, January 02, 2002 - 02:03 pm: The Nook|
well, of course, ion, you are correct.
no one should ever open a pc until it has cooled off to room temp.
|By nuecrew (Nue) on Wednesday, January 02, 2002 - 05:54 pm: The Nook|
I'm working on this problem of spores sticking to the inside of syringes. I believe there are two steps to the procedure of having a low concentration of free floating spores. One to the syringe and one to the liquid medium. I believe PF and others worked this out long ago.
|By quote: (Quote) on Thursday, January 03, 2002 - 03:07 pm: The Nook|
static charge ?
|By An guy (Boomer) on Friday, January 04, 2002 - 12:18 am: The Nook|
That's what I think. I wonder if experimenting with some kind of charged water, or lightly charging a syringe is worth experimenting with. Myabe just rubbing it with pos and neg static materials- I'm gonna try that with my next syringes.
|By ion ewe (Ion) on Friday, January 04, 2002 - 05:45 am: The Nook|
Well, yes, but I mean to let it sit longer than that so the jars inside will cool to room temp, also. No weirdness intended, Quo.
As for the sticking spores, try a wetting agent in the water if it really bugs you. A tiny bit of mild soap should do it, but I don't know if this will cause the spores problems...
|By An guy (Boomer) on Friday, January 04, 2002 - 07:40 pm: The Nook|
yeah, like concentrating gold, I see that, but wouldln't that be more for clumped spores?
I don't see how that would help the ones that are adhering to the barrel, that seems more a matter of charge....
Hell, when I get enough prints to try it though, I will- a tiny drop of soap...might not hurt, and if one used antibacterial soap, that might even help bacteria infections....;)
I'm also going to try, when I get some fresh syringes made, the rubbing thing with different materials, just to see. I've got a loupe that helps tremendously in examining syringes I found out.
But I need to do something. I've tested my first syringes, and several spots show no growth. Don't know if it's because I flamed the needle and didn't shoot enough in the spot to get through the dead stuff in the needle to live spores or what, but I know that not shaking the syringe often enough to keep a good homogenous suspension might have something to do with it, so anything I can figure out to promote said suspension i'll be looking for....