Karo tek stalled?

Nan's Nook : Archives : Liquid Culture : Questions?  : The Karo Tek Story : Q' about growth in karo tek : Karo tek stalled?
  Subtopic Posts Updated Creator

By onediadem (Onediadem) on Friday, December 21, 2001 - 01:00 pm:

I used a pf and hawaiian syringe to innoculate, and have found that after two weeks, the mycellium quit growing. Its been 6 weeks, and all that is in the karo jars is a quarter size mass. should I redo? they are still in the incubation chamber. I just wondered since the growth has stopped, if they wont be very effective innoculating jars. Any tips? should I redo? I have plenty spore syringes left.
Thanks all

By plinkerdink420 (Plinkerdink420) on Friday, December 21, 2001 - 02:02 pm:

is it still viable?... when was the last time you had a successful shoot off it?... if it's still alive i would just pop it in the fridge... id you haven't been swirling it from time to time, i would do that as well to help distribute the food and possibly oxygenate the water... it may have just ran out o food

p.s. how is your spine doing these days?

By onediadem (Onediadem) on Friday, December 21, 2001 - 02:56 pm:

I dont know if its viable or not plink, havent shot any of it cause it quit growing. I dont think Im gonna use it, its been 6 weeks incubating. I think I will do up some new, cant hurt. My neck is in bad shape. The operation was a success in the fact that it relieved the pain in my arm, but now I am having serious pain on the back side and up into my skull. Have called the Dr, and had new xrays done. Find out whats up on the 3rd. Other than that, Im doing really good, thanks for asking!
Merry HOHO

By Nan (Nanook) on Friday, December 21, 2001 - 05:45 pm:

If I were you I would go ahead and thrash those jars up and shoot them. Karo really should be drawn up and shot or removed from the incubatator no later than say day 14-16. Pure sugar cultures have no endurance. The goal is Karo or Dextrose is exactly what you obtained... A quick flash germination and a puff or two of mycelia. I generally shoot cultures started from spores at 7-10 days... Or into the fridge it goes.

By Karna (Karna) on Friday, December 21, 2001 - 10:36 pm:

I don't know exactly what you meant, but if your liquid jars have been incubating for six weeks, I'm very doubtful they will be viable. It is strange for contams to not show up in six weeks in a liquid culture.
If, however, they have been in the fridge for that long, they should be fine.
Here's how my liquid culture usually works. Mind you, I've never tried Karo.
After initial germination, the stuff grows out pretty quick for anywhere upto about 2 weeks. At this point, there is a puff ball like mass of mycleium visible at the bottom of the jar extending up the side of the jar about half or 3 quarters of the way to the surface of the culture liquid. From this point forward, very little visible growth takes place. At this point I tighten the lids, place them 2 ber baggie in a gallon size ziplock, suck all the air out and pop em into the fridge where they should remain good for a few months.

By onediadem (Onediadem) on Saturday, December 22, 2001 - 02:57 am:

Ok, I am gonna huck em. I have plenty of syringes to work with, so thats no problem, also, I have no more room for more jars right now, so I think I shall wait. Maybe redo the karo in a couple weeks. I dont want to waste anything on these jars that have done nothing in a month. They havent been in the fridge, but in the incubation chamber all this time. Thanks for the input, I really appreciate it all.
Live long and prosper,