Still more discussion....

Nan's Nook : Archives : Jars : Incubation & Storage : Nan's Nook Incubation Info : Still more discussion....
  Subtopic Posts Updated Creator
Are spawn jar air exchanges required?  3 12/20 07:27pm Ryan Waters
How important is temperature, really?  41 12/24 07:52pm Delekhan J. Delekhan
How important are incubators?  15 02/11 10:46am sammy

By mushmasterflex (Mushi) on Tuesday, November 27, 2001 - 06:39 am:

This is my first shot at growing shrooms and I followed the pf tek and used his pf spores. I steam sterilized the jars and inoculated them after cooling down. Its been over 5 days and I see no growth at all. Also, the jars were misplaced on a heating pad overnight and were warm to the touch and removed from the pad. I'd say they reached 90F but had no thermometer to be sure. How much heat can the spores survive thru? I'm not sure where I could have gone wrong in following PF tek except the heating pad incident. I dont see any discoloration or smell of the substrate so I think the jars are still sterile. I really dont know where to go from here... just throw a little knowledge this way please I'd really appreciate it.

By Tripster (Tripster) on Tuesday, November 27, 2001 - 07:09 am:

I'd say that you should wait a few more days to see if anything starts to grow, before you do anything else. What temp do you keep the jars at regularly? And how long do you think they were at the supposed 90F? did you pack the substrate down at all? There could be a lot of reasons as to why you haven't seen anything yet. Sounds to me like you should be ok as long as you followed the tek, however this is just my humble opinion. Good Luck.

By mushmasterflex (Mushi) on Tuesday, November 27, 2001 - 07:17 am:

The jars sat for about 6 hours or so at that temp. The ambient temp around here has been on the chilly side, 60-68F. I keep a blanket over the jars to keep them a little warmer. I did pack the substrate down and filled the remaining space with verm. I know I should give it some more time but I am worried that I killed the spores. Thanks for the input Tripster.

By ggg (Ggg) on Tuesday, November 27, 2001 - 09:33 am:



I think your dead at 106
while incubating try to stay warm not hot!
Keep it clean-

By quote: (Quote) on Tuesday, November 27, 2001 - 10:30 am:

that's too cold.
if you want those spores to germinate,
you'll need to get that temp into the low 80's and hold it there 5-7 days.

By wycrobro (Newman) on Tuesday, November 27, 2001 - 03:48 pm:

From what i have read they wont germinate at all at that temp. Try an incubator there are good cheap teks in the arc.
Or try putting the jars in some sort of container and put it on the heating pad. Start low then turn it up as needed? I think this should work.
good luck

By Snoopy (Snoopy) on Tuesday, November 27, 2001 - 07:37 pm:

Also....... wrapping a blanket around them won't help inless you have a heat source within.. THe blanket will retain heat, but if there is no heat in there in the first place than it isn't doing much good other than keeping the light out...
Food for thought

By quote: (Quote) on Tuesday, November 27, 2001 - 07:56 pm:

interestingly enough, the jars do generate some heat of their own [once germinated].

By mushmasterflex (Mushi) on Tuesday, November 27, 2001 - 08:32 pm:

Thanks for the advice guys. I just got a styrofoam box which I think will work well as long as I get a heat source inside. If anyone could suggest where I might be able to get a thermometer, please do. The local supermarkets and drug stores don't seem to have any.

As for the blanket idea... It was just plain thinking that blankets keep me warm at night. Stupid me.

By mushmasterflex (Mushi) on Wednesday, November 28, 2001 - 12:05 am:

Okay, I think I got the incubation problem covered. The styrofoam box plus a set of 100 white christmas lights seems to be a good mix. I still dont have a thermometer to be sure but its warmer than the ambient temp. If anyone sees any faults with my makeshift incubator please do let me know. Also, if any other information is available on how much heat spores can take, please post. I know there is only one way to find out if my spores died but if any other info is out there, I'd be more than happy to know.

By Lichen (Lichen) on Wednesday, November 28, 2001 - 12:08 am:

well, a simple thermometer can cost you as little as $3. Get one. It's the only way to make sure your incubator is right. The problem with incubators is the ambient temp outside the box can affect the temp in your box.

Spores should not be stored any higher than 75F

By jim brown (Shrhobbyist) on Wednesday, November 28, 2001 - 09:02 am:

If you can't find a thermometer you can't be looking too hard. Do you have any '-mart' stores near you?

By any chance is Ggg Greenthumb? If so, why the new name?

By ggg (Ggg) on Wednesday, November 28, 2001 - 09:24 am:

sick of typing that name out.
I used to be able to grow things-
Won't be able to prove that again until spring!!


By jim brown (Shrhobbyist) on Wednesday, November 28, 2001 - 09:34 am:

What will you be growing come spring, if you don't mind me asking? And what do you mean by "used to be able to grow?" Is it a problem of location or did you lose the touch. Oh well, welcome to the Nook Ggg. Thanks for all the links!

By ggg (Ggg) on Wednesday, November 28, 2001 - 10:32 am:

Full blown 1/2 acre veggie garden.
Hot peppers out the ass-
I make my own hot sauce and can my veggies so I hate winter. I can't grow shrooms cause my designated grow spot is too dirty.

Thanks for the warm welcome Jim
CU soon

By jim brown (Shrhobbyist) on Wednesday, November 28, 2001 - 10:50 am:

Me and my girlfriend grew oregano, parsley, chives, basil, tomatoes, beans and garlic this summer. Enough spices for about 3 years of heavy use. We dried the spices and that made them suck! We froze the rest and that keeps them near perfect. But, damn, we lost a lot, already done with the parsley, which we both love. Oh well, we had fun doing it and will have fun doing it again next summer. My folks have had there gardens featured in a few national magazines. They built a greenhouse 2 years ago to keep stuff going all year long. Man, I could use that greenhouse!

By wycrobro (Newman) on Wednesday, November 28, 2001 - 03:35 pm:

Well mushmaster. the lights in the incubator might be a problem?
I would suggest the styrofoam tek in the arks Im not sure of the link but im sure someone can post it for you.
As for the thermometer I use one of those floating fish tank ones, I'v cut a hole just big enough(make it small and force it through) the thermometer is round and goes half way in so you can read the temp with out opening the incubator.

By mushmasterflex (Mushi) on Wednesday, November 28, 2001 - 04:36 pm:

Alright a have a mini update. I got me a cheapo thermometer after a long search and 100 christmas lights are a little too hot for the mycelium. The temp reached about 100F and now I am @ 50 lights @ ~80F. I will continue to tweak it til it reaches about 85F, sounds good? And another possibly good note, I see some white growth in one of the jars in one of the innoculum sites. I hope they pull thru.

By quote: (Quote) on Wednesday, November 28, 2001 - 04:48 pm:

sounds like you're on your way...

By wycrobro (Newman) on Wednesday, November 28, 2001 - 05:42 pm:

What about the light? unless he is using your neg tek or going for invetro pinning wont this be a problem quote?

By mushmasterflex (Mushi) on Wednesday, November 28, 2001 - 05:59 pm:


I'm not sure what I might do as to after if the cakes colonize. Invitiro sounds good but a bigger yield for christmas shrooms sounds muy bueno. I'm still contemplating that... or maybe I'm counting eggs before they hatch. As far as my knowledge goes, light won't hurt the mycelium while its colonizing, so I *think* I'm safe.


That is one trippy picture. Might you happen to have one in a bigger format so I could put it on my desktop?

Thanks to everybody for their assistance and I will keep you guys posted.

By Ron (Clubbenguy) on Wednesday, November 28, 2001 - 07:06 pm:

Good luck, I go to retile shops for my thermometer/humidity gauges. They come in a two pack you can just stick to the inside of the walls of your grow chamber/incubator.

By mushmasterflex (Mushi) on Wednesday, December 05, 2001 - 11:54 pm:

Hello again, I have a little update for those who might care and another small question. My styrofoam incubator is doing the job but it is doing it rather oddly. There is enough space in the container to fit 8 jars on the bottom and plenty more room vertically. So i stacked the jars 8, 8, 4 which gives a total of 20. The thing is that the bottom 8 have colonized much faster than the other 12. I'm not freaking out or anything but just damned curious. I'd say the bottoms jars are about 70% and the rest are barely starting out @ 20-30%. I got the temp in there @ 86F which seems optimal. The source of the lighting are christmas lights and I have them running in between jars and evenly distributed. I was thinking that heat rises and it would be cooler at the bottom of the incubator. Would that mean I should be lowering the temp? Eh... I'm confused.

Above all, I'm happy my spores didn't die and hopefully I can look forward to X-mas shroomies.

By SYDYSTYK (Addict) on Wednesday, December 05, 2001 - 11:57 pm:

rotate the jars then-top to bottom

By quote: (Quote) on Thursday, December 06, 2001 - 01:12 am:

but it's best not to wonder about somethings too much.
moving the jars around sounds like a good option.
btw, i don't have that pic sized,
you should be able to set it as wallpaper, just right-click on it,i think, but i'm no 'puter xpert.

By mushmasterflex (Mushi) on Thursday, December 06, 2001 - 10:24 pm:

Another quick question... The bottom jars are reaching full colonization. Should I take the tape off the innoculum holes? Let the mycellia breathe and promote growth? Thanks in advance.

By Nan (Nanook) on Thursday, December 06, 2001 - 11:28 pm:

You can remove the tape. Some people flip the jars. If the jars are going good it's personal preferance.

By Hell No (Mr_Rogers) on Monday, January 28, 2002 - 10:19 pm:

when the shrooms are colonizing in the jars, what will happen if the temp is around 65 instead of 75?

and if it wont work at that low of a temp, how should i warm the area around the jars efficiantly?

also once the jars are innoculated, I keep them in complete dark while they colonize?

By C K (Phrozendata) on Monday, January 28, 2002 - 10:48 pm:

They will just colonize slow.

They *should* be 80-85 degrees. Remember the jars are usually 2 degrees warmer than the surrounding air.

By Brettiejams (Brettiejams) on Monday, January 28, 2002 - 10:52 pm:

Give em light at about 75% colonization..... unless you plan on fruiting in-vitro, in which case exposure to light throughout the entire colonization process is recomended.

By Hell No (Mr_Rogers) on Monday, January 28, 2002 - 11:02 pm:

wow everyone here is so helpful. This is going to be my first attempt at growing shrooms.

Right now it is winter, how do i keep the jars at 80 degrees?
I only keep my house at like 65, 50 during the night. I was thinking of putting them in that little closet where my boiler is, but its off at night. Is there some sort of safe heat pad i can get inexpensivly for the jars?
thanks for the help.

By Doc Thirst (Doc_Thirst) on Monday, January 28, 2002 - 11:06 pm:

Yeah, you can get a heating pad at walgreens for like 15 bucks

By Brettiejams (Brettiejams) on Monday, January 28, 2002 - 11:31 pm:

Check out this link for more ideas... Heater

Putting your jars in a cheap strofoam cooler or a nicer thermos cooler would be a good start.

Then, just add a stable heating source.

By 420 (Mr_Rogers) on Tuesday, January 29, 2002 - 02:17 am:

eh we dont have any walgreens in this state, what type of store is it?
also can someone point me to a link of what a heating pad looks like or post a picture?

By Martaxus (Martaxus) on Tuesday, January 29, 2002 - 02:47 am:

I've another way, though it hasn't been tested on live shrooms yet. If you leave the colonizing jars in the box they came in, 6 small white x-mas lights will raise the temp to around 80F. You'd have to put in a thermometer to test before use.

To do this, cut some cardboard that will fit inside your box & sit on top of the jars. Then tape the string of lights to that so that you can close the box normally. The cardboard with lights could then be lifted out for working on the jars. Done efficiently, you could light/heat 4-6 boxes this way.

By Hatcher (Hatcher) on Tuesday, January 29, 2002 - 04:38 am:

Pet store:reptile under-tank-heaters are fantastic...getting ready to build the styrofoam cooler w/aquarium heater in jar o' water incubator tek myself.. I use the UTH's for my grow units. Target temp for fruiting: 75F.

Birthing : Double-End Casing : Growing Invitro : Dunking After Birth : Shroom Glossary

By Nick Nick (Heytheresmoker) on Tuesday, February 05, 2002 - 03:30 am:

im looking to grow these mushrooms the fastest and easiest way possible..i have a incubator that is nice and big that is used for incubating reptile eggs..will this work if i keep it at 85 degrees all the time??how much will this help speed wise..and is the PF tek fast or is there a faster way to get mushrooms?? im growing PF and Huawian(spelling) thankx

By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 03:38 am:

Yes, that will work just fine. Maybee put a towel or something under your jars so they are not sitting directly on a heating source.

Stick with the P.F. tek before you get too worried about speeding things up.

The P.F. tek is the essential tek for beginners.

Succeed first, cut corners later.

By Nick Nick (Heytheresmoker) on Tuesday, February 05, 2002 - 03:43 am:

the heat source is above the long till i can birth the cakes when in the incubator???

By Brettiejams (Brettiejams) on Tuesday, February 05, 2002 - 03:47 am:

Should wait until about 5 days after complete colonization, or until you see your first pin(s).

Usually about 28 days or so from innoculation with a PF syringe.

By Nick Nick (Heytheresmoker) on Tuesday, February 05, 2002 - 04:01 am:

ok after i inject 1 cc into a jar do i have to burn the tip to make sure its not contaminated or just do all my jars in one shot..i also have a crock this like a pressure cooker??can i use it to sterlize my jars??

By plinkerdink420 (Plinkerdink420) on Tuesday, February 05, 2002 - 04:09 am:

i think that unless you touch something with the tip of the needle other than the inside of your jars, then you don't have to flame it... i usually do it between every jar, though just 'cause i'm paranoid

i might be wrong, but i don't think that you can use a crock pot to sterilize your jars... i think you will have to either steam them or pressure cook them

By Nick Nick (Heytheresmoker) on Tuesday, February 05, 2002 - 04:14 am:

i have never done shrooms before because like you im paranoid about things to so i wanna grow my own..what is it like(if able to explain)??and is it true that you can die from it or is that just something your teacher tell you so you dont do it??

By plinkerdink420 (Plinkerdink420) on Tuesday, February 05, 2002 - 04:21 am:

dying from eating mushrooms? i don't know... don't seem possible... if it is, i'm sure you would have to eat quite alot

as far as the experience, it's impossible to explain... kinda like lsd but kinda not... just different

By Nick Nick (Heytheresmoker) on Tuesday, February 05, 2002 - 04:37 am:

i cant wait.. i never did LSD but i have heard both good and bad things about much should i take the first time??and should i dry them or eat them fresh??whats the most potent strain of shrooms?

By dan k. (Cheerio) on Tuesday, February 05, 2002 - 04:55 am:

You can die from eating the wrong mushroom, or ingesting a contaminated mushroom.

Straight from PF Tek
Contamination - a word of warning

Psilocybe Toxicity Information - The original copy of this document was be found at - (Just a note, but this document is no longer there) The following are common symptoms reported during a typical intoxication.

Onset: dizziness, giddiness, nausea, weakness, muscle aches, shivering, anxiety, restlessness, abdominal pain.

Not to metion the poisonious alakaloids in the wrong type(of some picked) mushrooms. This you dont have to worry about if growing your own.

By Ron (Clubbenguy) on Wednesday, February 06, 2002 - 05:38 am:

I used a crock pot to sterilize, anything with a good fitting lid. I just fill my pot with 2-4 inches of water (bout 1/2 way up the sides of the jars) and boil for one hour. Good luck.

By jim brown (Shrhobbyist) on Wednesday, February 06, 2002 - 11:08 am:

Hey Nick Nick; when teachers or policemen tell you about drugs they are lying to you. I hope for your sake you are not fooled by some full of shit teacher who tells you stuff (lies) about drugs while in fact this teacher has never done drugs nor has heard anything about drugs outside of shameless urban legends (My friend's friend did acid and now he thinks he's an orange). If you believed that shit you would not be here. Read this board (Nan's Nook), see Erowid for some real information. Next time a teacher tells you about a student of there's who died because he took acid and taught he could fly, tell them they would have made a five star Nazi. Propeganda makes me sick.

By gnas (Psystep) on Thursday, February 07, 2002 - 07:51 pm:

Hey guys, if your growing invitro or have a set-up inside your room, which includes a computer, one way to raise the temperature is to always leave your computer moniter on. (with a screensaver ofcourse so you don't burn the screen up)
I found this the best way to warm up my room since I don't have temperature control here and no incubator.
Better yet, set your jars on top of the monitor with some kinda barrier.

By Ink drop (Headrastaman) on Sunday, February 10, 2002 - 08:38 pm:

I was just wondering how exactly i should keep my jars at a constant temp at around 80 deg. what types of methods should i use? right now i use an aquarium heater set at 80 degrees and i float the jars in water.. i dont like this method, but it works, i'm just looking for something different thats all.... any ideas? thanx... i was thinking of getting a heating pad u use for hydroponics... but i'm not sure how well that'd work... ok thanx for ure help..

By quote: (Quote) on Sunday, February 10, 2002 - 10:13 pm:

try puting that heater in a gallon milk jug full of water and sit that inside a plastic box with your jars.

By Ink drop (Headrastaman) on Monday, February 11, 2002 - 10:00 am:

damn... thats a good idea

By nuecrew (Nue) on Monday, February 11, 2002 - 04:12 pm:

I use a oil filled radiator style space heater with a small fan plugged into a "variac" adjustable transformer. I have them in a 100 square foot room(10x10'). I run them both 24/7 and keep the temp. of the room around 76*(76* ceiling,72 floor). Without the fan the top of the room gets very hot while the bottom stays cool. The room is used for all stages of growth. Innoculation, growing and drying. My electric bill has gone up maybe $10 a month. I have tryed localized heating and usually end up with harmful drying. I am a beginner and am still learning though.

By Richard Warren (Tex) on Tuesday, February 12, 2002 - 04:24 am:

I just mixed the cake mixture from the PF Tek and injected the spores. How much air should I allow in the jars while during incubation? I have duck tape over the wholes in the lids from sterilizing, should I leave those on there or take them, what? Thanks for the help!

By Brettiejams (Brettiejams) on Tuesday, February 12, 2002 - 04:57 am:

Duct tape is not real good... too much adhesive, adhesive that gets soft at incubation temperatures.

Masking tape works, and that plastic micro-pore tape works real good too.

As fan as whether or not to use tape, some do, and some don't. Both ways work. IF you use tape it should come off at about 50% colonizaton.

Just a little aluminum foil over the top of the jar works real good.

Next time.. avoid the duct tape though.

For more about Tape, check out that link.