|By Ryan Waters (Zerogravity) on Thursday, December 20, 2001 - 01:21 am:|
Should our spawn jars be airtight or vented with polyfilled holes/filter disks?
(Looked in the archives but I somehow missed this answer.)
|By relic (Relic) on Thursday, December 20, 2001 - 05:20 am:|
they need to be vented somehow for proper colonization. either filter disc, polyfil, or inverted lids taped to the bands and losened(sp?).
|By Ryan Waters (Zerogravity) on Thursday, December 20, 2001 - 07:27 pm:|
Thanks Relic for your good advice. We are trying to err on the side of caution, so what is your opinion of the following thoughts.
Our incubation cabinet has a 4" high-CFM fan (greater CFM than a computer case fan)necessary to achieve even temperatures throughout. Prior to the fan we had variances of 9 degress from top to bottom (which is 5 feet). Now there is no variance. The cabinet is in a small HEPA environment.
We are nervous about circulating (albeit slow) air when our "babies" are at one of their weakest points of growth.
Question: If the polyfiber wicks moisture from the damp grain when agitating for even myc growth, will this wet wick attract cobweb mold in the stagnant air underneath the 2 layers of foil?
Your suggestion to allow loosened lids lead us to believe that there will be more than 3 microns of jar exposed without a barrier...correct? We see it hard to make sure all of our jars would be the same degree of tightness...some too loose and others essentially sealed.
What are your thoughts re a ball of fiberfill in the neck of the jar like the type you find in vitamin bottles? Or what about 2 layers of filter disks on the top & bottom side of the lid? Our reasoning behind these is to reduce the chance of an exposed wet filter to the air.
Also when the doors are opened, should we spray the air with a 10% bleach solution?
Or are we just myco-hypochondriacs?
* This sounds fine, no need to spray bleach