Nan's Nook : Archives : Liquid Culture : Honeytek
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Easy No PC Needed HONEY TEK  29 11/08 07:34am  
Honeywater mycelia tek  15 08/23 12:18pm Admin
Is this too good to be true?  10 01/17 03:25am Mr. B

By Coolmist (Coolmist) on Tuesday, July 31, 2001 - 02:41 am:

Honey Tek

a foaf sent me this pic of the honey tek. a week after knock up.

By Xpeanut (Xpeanut) on Wednesday, August 01, 2001 - 03:36 am:

Tnx Vitti for getting me on! Honey tek is the cat's meow for me, although I have found that I like molasses better. Get the non sulphured type. I have been doing some experimenting and prefer honey or molass over brf in water.

By Mushy (Mushy) on Wednesday, August 01, 2001 - 12:01 pm:

tort, mycellial water is used to speed up jar colonization. With a tiny bit of spores, you can get a jar like this. This jar would make like 4 syringes, at least. Since the spores are already germinated, you will get growth in the jar within a day. Full colonization within 2 weeks for sure.

Also, if you take a chunk of shroom or mycellium from a fruiting cake to innoculate the honey water, you will have an isolated, fruiting strain. Much higher yield by using this method.

By J Stanton (Khanif) on Monday, August 13, 2001 - 02:54 pm:

Have been seeing the same cloudy precipitate in the Honey/H20 jars IMMEDIATELY after PC'ing for 30 min @ 15 psi. Since there's only honey & DI H20 in the jars, assumed the precipitate must be carmelized sugars. Can these jars with precipitate in them be inoculated successfully? Or should new honey /H20 jars be prepared? Anyone know how far the total PC time can be backed off without compromising sterility of the jars?

By afg on Friday, August 03, 2001 - 07:30 pm:

hey coolmist, do you know your foaf's recepy / procedure.

i've seen it done before but the results didn't look quite that nice. i'm thinking that during the pc process much of the sugars got carmelized r somthing... what i saw, even in the control jar, was a fine dusting of small white precipitant in all of the jars, it took 2 weeks for the innoculated jars to about 1/2 the growth of the jars in your origional picture, but it did prove to be uncontaminated n the mycelium innoculated jars did colonize n fruit just fine. next time i'll prolly just pc for 20mins, but curious bout your foaf's methods.

the procedure i witnessed:
1 tbsp honey dissolved into just under 1pt of tap water then pressure cooked for 30mins @ 10psi(da pc only goes to 10psi)

jar lid had 1 hole packed full of polyfill.. trimmed, duct taped down to outside of jar, layer of plastic wrap on the inside side of the lid to keep the polyfill from gettin wet, oh yea, there was also some small shards of glass inside to help break up the mycelium b4 fillin up da syringe. i gotta find a hobbyshop n make one of those super-duper mycelium syringes that.. (who was it) Rochester or Nanook posted on the old board.

By Nanook of the North (Nanook) on Monday, August 13, 2001 - 05:08 pm:

Sediment will drop out of honey when it is pressure cooked. You also get scum. Honey contains protiens which denature (cook) when it is heated,
especially under pressure. The denatured protiens produce a whitish haze and some precipitate. The wax forms a scum. The tint of Honey Tek makes carmelization difficult to see...

Nothing you can do about it (switch to Karo or Dextrose perhaps), but it will still work fine.


By Fungusflipper (Fungusflipper) on Tuesday, August 14, 2001 - 02:06 am:

Mycellium doesn't like caramelized sugar. If you are gonna pc, you need to use dextrose powder added to water. It will not caramelize, according to Nan, and I haven't seen it do so yet. As always, report your findings here to help others.


By Nanook of the North (Nanook) on Tuesday, August 14, 2001 - 03:13 am:

Carmelization does not normally take place in dilute sugar solutions in a pressure cooker unless you are doing something wrong. Jars of sugar solution should not be placed directly on the bottom of the pc. Use a standoff plate, a handtowel, something. Other than that you should be OK.

The temperature inside a pc at 15lbs steam is about 250*F, not hot enough to carmelize a dilute dextrose solution that is for sure. They remain clear as glass after 30-40 minutes under steam and it makes spotting germination and contams a breeze. It is so cheap and easy to get: Ebrew.com, Beer Additives, Corn Sugar/aka Dextrose).

Get a pound, Ebrew are good people and you may find other items of interest there as well.

Some sugars in honey may degrade, but there is plenty of sugar types present in honey, and I am sure it will remain sweet and grow mycellia.


By Purge (Purge) on Tuesday, August 14, 2001 - 01:41 pm:

ebrew lists dextrose as corn sugar. would corn syrup (karo syrup) work the same way?

By Nanook of the North (Nanook) on Tuesday, August 14, 2001 - 07:43 pm:

Clear Karo would work fine... But I am not sure what the syrup equivilent is (when compared to dextrose powder) since it contains some water. Still I imagine that a teaspoon of syrup in 75cc of water would work fine. The syrup is pretty thick.


By Karna (Karna) on Tuesday, August 14, 2001 - 08:36 pm:

nan, ever try brown rice syrup in water as a culture medium? I saw some of this stuff in the organic foods section and was contemplating giving it a try. I'm not sure what they did to arrive at it - prolly a process similar to making corn syrup from corn. Any thoughts?

By atk on Tuesday, August 14, 2001 - 09:37 pm:

do you guys highly recomend using this honey tek? colos fast but im worried about contams. looks like it saves alot of money and time also. scared to always take the next step, prints still havnt been made, dont know why i do this but ill change soon hopefully.

By Nanook of the North (Nanook) on Wednesday, August 15, 2001 - 03:22 am:

Karna, I like dextrose (aka: corn sugar) because it is clear. Contams and off colors are much easier to spot in clear solutions.

There is more nutrition in grain extracts and cruder sugar products I am sure, just about anything would work as mycellia does not need anything other than a simple sugar to get started. I bet a spoonful of table sugar in 75 ccs of water would work fine.

But I like it clear so I can glass it carefully with an eyeball. Nutrition is something I worry about when I work on fruiting substrates.

ATK, yeah this is a simple, and highly recommended proceedure for producing inoculum.


Shroom Glossary

By Martaxus (Martaxus) on Saturday, December 08, 2001 - 09:09 am:

Is it possible to reliably check for carmelization of sugars when preparing honey tek without testing/wasting spores?

I've heard people recommending lower sterilization times to avoid this. Any ideas?

By Trollhunter (Trollhunter) on Saturday, December 08, 2001 - 03:40 pm:

hmmm as many times as i have done the honey tec never really thought of that. but i just leave it in for 15 mins at 16 psi . seems to work every time . but i would think if it did go wrong . you would see the sugar come out of solution , and thus you would see shit floating around .but dont mark my words on the part about the sugar .

By Mr. B (Argonaut) on Saturday, December 08, 2001 - 08:22 pm:

If the solution is darker comming out of the PC than it was goin in, you have some degree of carmelization...

By Mr. B (Argonaut) on Tuesday, December 11, 2001 - 05:52 pm:

Little round, fuzzy floaters about 1/8 - 1/4 inch diameter. No funny smells. This is Honey innoculated on 11-8

C:\Galleries\Main Gallery
C:GalleriesMain GalleryMycell.JPGMycell.JPG

By jared (Jared112) on Tuesday, December 11, 2001 - 06:40 pm:

Yup there it is again. No doubt about it, but 11-8, that was ages ago. What temp have you been storing the jar at?

By Mr. B (Argonaut) on Tuesday, December 11, 2001 - 07:18 pm:

My mistake it was 12-8 was actually THREE days ago not quite ages ago... A simple typographical error...I have had the jar @ 81 deg F for about 16 hours a day. At night it gets to around 71 deg F.