Mixing Strains

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PF's Experiment  -    

By jared (Jared112) on Wednesday, October 31, 2001 - 06:48 pm: The Nook

I just wanted to know if I would be fucking anything up by trying to grow a few cakes of PF and a few cakes of B+ in the same terranium? I would like to do this just so that I can see the differences between the two side by side.

By Brettiejams (Brettiejams) on Wednesday, October 31, 2001 - 07:20 pm: The Nook

Fruiting two different kinds of cakes in the same terrarium is A-O.K.
By UnkyHerb (Notapplicable) on Tuesday, November 20, 2001 - 09:06 am: The Nook

What would happen if you took half of an A strain cake, then crumbled it and put it in a pie pan, with half a cake of B strain already in there, then mixed it up and let it colonize... think the mycellium would work together?

By quote: (Quote) on Tuesday, November 20, 2001 - 02:20 pm: The Nook

don't mix them together, they'll fight and yields will suffer.
but you can case them in the same pan, just give each strain its' own space.

By Kman (Kman) on Sunday, December 30, 2001 - 09:05 pm: The Nook

Hey I have 3 different strains on the go right now, I have EQ's, Plantasia and some unknown cubensis. I have 4 jars of rye colonizing and 9 pf style jars colinizing right now. What I plan to do is make flat cakes out of them, I want to know if these strains can be mixed together in the same flat cake without any ill effects? Also can the rye and verm/brf be mixed in the same tray if absulutely necessary? Or would it be best to leave them seperate?

By ShrooMaster (Shroomaster) on Sunday, December 30, 2001 - 09:28 pm: The Nook

man this is a good question. according to pf the 2 strains would combine and fight it out and the winner would fruit but only on the portion of the cake it was inoculated on and spred across itself. i guess it would be a idead if you absolutly hat to cover somethng up quick but if youve got enough to do them all right, do it right.

By quote: (Quote) on Monday, December 31, 2001 - 01:02 am: The Nook

i've tried it b4, what seems to work best is to place each separate strain in its' own area, and not to intermix them. then they'll each grab their own sector, and fruit.
whereas if you mix them up, the strains will fight for dominance, decreasing overall yields.

By Kman (Kman) on Monday, December 31, 2001 - 01:13 am: The Nook

so could I make 2 different sections in a tray, by either seperating the two sections(leave a gap between the 2) or maybe a tin foil divider or something? The problem is I got my labels mixed up on 3 jars of rye 2 eq's 1 plantasia whats the min. amount of spawn I could use for a 11"x8" pan? the rye jars are pint jars filled half full? But I should definatly NOT mix the strains?

By quote: (Quote) on Monday, December 31, 2001 - 02:35 pm: The Nook

correct, no need for any divider though, just keep them un-mixed.
layer your spawn 1/2-3/4 inch thick.

By Kman (Kman) on Monday, December 31, 2001 - 05:42 pm: The Nook

Thanx quote! Thats Y I come here, you guys always amze me with the straight facts! I know I wouldn't be able to get hard answers anywere else, you guys are da shiznit

By nuecrew (Nue) on Thursday, January 03, 2002 - 09:30 pm: The Nook

Heres a Hawaiian and a flush of PF's *SUPERSTARS* growing side by side each with there own pint jar of rice below.Haw. & PF

By quote: (Quote) on Friday, January 04, 2002 - 02:19 pm: The Nook

nice photo,

By plinkerdink420 (Plinkerdink420) on Saturday, January 05, 2002 - 07:10 am: The Nook

that photo is simply amazing nuecrew

By nuecrew (Nue) on Saturday, January 05, 2002 - 09:02 pm: The Nook

I've tested quite a few different strains now and out of six the PF classic is still the best performer in flush yield and potency(although the Equadors were quite strong). I have my first B+ coming in soon, I've heard they are biggies.

By Bjsloan (Bjsloan) on Saturday, February 02, 2002 - 07:27 am: The Nook

What if substrate were innoculated with 2 different strains. Such as, innoculate one side of a cake pf style with one strain, and the other half with a another. I am wondering what would occur.

By Hatcher (Hatcher) on Saturday, February 02, 2002 - 07:36 am: The Nook

Don't do it.

By Brettiejams (Brettiejams) on Saturday, February 02, 2002 - 07:50 am: The Nook

You could prolly get away with doing a half and half cake.

The two strains will sector off though, and if the sectors are not large enough, the fruiting won't be good.

Two different strains will not combine to form a single colony.

By Some Body (Texasshroomer) on Sunday, February 03, 2002 - 12:33 am: The Nook

I hope this isn't a dumb question, but...

Could two different fungal organisms of the same species but of different strains (cubensis mycelium) reproduce sexually in substrate?

I know that fungi can reproduce sexually and asexually. So how could this happen in a lab? Could it happen in the jar?

Hopefully I'm not way off track. It's been years since my last biology course.


By jim brown (Shrhobbyist) on Sunday, February 03, 2002 - 11:04 am: The Nook

No, if that could happen every grower could have there own race. It's a fun thought though. You could make a kit where you mix spores and come up with a mushroom no one has ever seen.

By Some Body (Texasshroomer) on Sunday, February 03, 2002 - 06:14 pm: The Nook

Quote from Biology site about sexual reproduction of Basidiomycetes (mushrooms):

Sexual reproduction occurs when haploid nuclei (having a single set of chromosomes) in basidia (club-shaped structure typically bearing four basidiospores at the tips of minute projections along the gills of mushrooms) fuse, then undergo meiosis (which produces haploid cells with less chromosomes). The resulting haploid basidiospores germinate, and hyphae of compatible mating types fuse to regenerate the dikaryotic state.

I added the parenthesis for clarity.

Ok so now that I understand how it works. Has anyone accomplished this in a laboratory setting?


By Erik B. (Tryptamine) on Wednesday, February 06, 2002 - 06:12 am: The Nook

First of all this is possible and assuming the two strains you used are both pc you could get a fruit that is a cross and it will be viable as well. Basidiomycetes reproduce exclusively through the sexual process unlike other fungi. all it takes is two hyphae of different mating types (+ and -) to meet and fuse (plasmogamy) and then the dikaryotic hyphae turns into the fruit. most species of pc are close enough to interbreed. It won't work one hundred percent of the time nor even close but it will work eventually.

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