|By Tripitydoodah (Tripitydoodah) on Wednesday, November 21, 2001 - 08:52 pm:|
quote, i saw a picture that you posted a while back where someone was growing shrooms out of a tupperware container. a foaf tried the flat cake tek and he got one flush out of it then lost it to contamination. it seems better to just leave it in the container and fruit it that way. if you have any info on this tek please help out. he has got an albino cake in a pyrex baking dish now and i was just wondering about if he should just keep it in there. thank you.
|By quote: (Quote) on Wednesday, November 21, 2001 - 09:03 pm:|
sure, why not ?
the container will hold the casing as well as anything else.
and, since i'm assuming it's transparent,
you'll get extra fruitbodies down under.
just pop the 'cake' out, pick them, and return to continue the cycle.
|By Tripitydoodah (Tripitydoodah) on Thursday, November 22, 2001 - 04:27 am:|
|By Brad (Raze) on Sunday, November 18, 2001 - 06:53 pm:|
I grabbed 6 half pint ziplocs today, just wondering if its safe to PC them?
|By Nan (Nanook) on Sunday, November 18, 2001 - 07:10 pm:|
Ziplocks are sterile out of the box. They will melt in the PC. If you need bags for PC work, get oven bags.
|By Brad (Raze) on Sunday, November 18, 2001 - 07:17 pm:|
No no, not bags... The ziplock disposable tupperware things
|By An guy (Boomer) on Sunday, November 18, 2001 - 07:29 pm:|
Glad makes them too- those hard-walled lock lid containers. I know they have some heat resistance, but not sure what/how much.
Are they those oven ones? It should mention somewhere what they can and can't take and if not, I would go back to the store where I got them, and look at some other ones, see what they say- they're all pretty much the same technology, so what you can do with one, you can most likely do with another.
An alternative is to do some plain old fashioned experimenting- toss one in the pc and see what happens- I dont' think they're that expensive, are they? If you do though, try putting a flat, non-metallic surface under it, and let it cool in the pc before you touch it, let it harden again.
|By Nilli Willis (Procell) on Sunday, November 18, 2001 - 07:53 pm:|
I have a set of ziplock containers runing right now. They survived the PC, just make sure they don't touch the side of the pot during heating. I also put them in individual ziplock bags to aid sterility.
|By Brad (Raze) on Sunday, November 18, 2001 - 08:02 pm:|
Thanks procell, that pretty much clears that up.
I did them in the microwave anyways today, made up 4 conainers of 150mL (with 2 tsp of dextrose).
Gonna let them sit a week or two to see if they contam. I have two good ones on the go anyways.
|By Nilli Willis (Procell) on Sunday, November 18, 2001 - 08:04 pm:|
Oops, guess I should have said, "I put them in bags AFTER cooling". I also didn't have the heat on max high when in the pc, still sterile but a little longer with a slightly lower flame.
|By Brad (Raze) on Sunday, November 18, 2001 - 08:29 pm:|
|By Nilli Willis (Procell) on Sunday, November 18, 2001 - 09:15 pm:|
There was a little mis-shaping of the lids where they touched the pot sides, so putting them in baggies helped maintain sterility. No contamination as of yet so I think it worked.
|By relic (Relic) on Monday, November 19, 2001 - 04:10 am:|
i've done many ziplock container cakes. they survive the p.c. fine, but the lids loosen a bit. i wrapped the lids with foil.
|By Cragith Kilbonith (Kilborn) on Monday, November 19, 2001 - 04:52 am:|
relic but they turn out fine? what are the contam rates, wana share
|By quote: (Quote) on Monday, November 19, 2001 - 01:41 pm:|
i've used them, too.
they work ok, but one must be careful when moving, stacking them, as they are flexible.
|By relic (Relic) on Monday, November 19, 2001 - 07:08 pm:|
ditto, what quote said. watch those lids. i never had any problems using them. if i want to make cakes, i still use them. i use 12 inoc. holes 2 on each side of the lid near the corners and 4 evenly spaced into the middle of the lid. i use the square ones. they colonize at least as fast as w.m. 1/2 pints and with more substrate w/ an incredible success rate, for me anyway. i initially tried microwaving them, i steamed them, and p.c.'d them. the microwaved ones contam'd(only nuked them for 3 min. though and never tried again). the ones that were steamed worked great and the one's that p.c.'d worked great. lid got looser p.c.'ing than steaming. i wrap foil over the lids before sterilizing and return the foil after inoculation.
|By UnkyHerb (Notapplicable) on Tuesday, November 20, 2001 - 09:11 am:|
Heh, i just bought 24 of those ziploc ones too.. guess what i'm going to try ;) One problem I see, it when you heat plastic, any germs and whatever adhere to it permanently... So make sure no air gets inside :o
|By quote: (Quote) on Tuesday, November 20, 2001 - 02:22 pm:|
|By Rick (Npno) on Tuesday, November 27, 2001 - 02:55 am:|
I was just wondering is there are any other ways to sterilize jars other than boiling or using a PC.
|By quote: (Quote) on Tuesday, November 27, 2001 - 11:10 am:|
some have used microwaves.
|By Rick (Npno) on Tuesday, November 27, 2001 - 09:26 pm:|
can anyone give me some tips on how to use a microwave for sterilization
|By Lichen (Lichen) on Tuesday, November 27, 2001 - 10:26 pm:|
I have not had good results with it, but I only tried it a few times, in a few ways...you might be able to do it effectively, but you can't use metal, obviously, and plastic becomes too pliable and collapses, unless you bought a special container. Then, once the sterilization is complete, your container will suck air, causing failure. If your vermiculite layer on top inside can hold out the contams that get in this way, good. But that's been my problem with it. Every time I tried this, I ended with contams.
You might try a glass casserole dish, put the substrate in, cover with 3/4" of dry verm, nuke for five minutes or ten, and leave it in the microwave till cool. I never tried this, because of the failures, it just didn't seem to be working out.
Why are you doing this, if I may ask? Is there a reason you cannot do it in jars with steam in a pot/pc? If there isn't, go with the tried and true--steam
|By Lichen (Lichen) on Tuesday, November 27, 2001 - 10:39 pm:|
if you have a pc, it only takes 30 minutes to steam a batch of jars. Someone else have an idea for microwave sterilization? Like I said, it's not the best way to go
|By quote: (Quote) on Wednesday, November 28, 2001 - 01:40 pm:|
the gladware microwave-safe disposable containers work ok for 'waving.
but you'll need to experiment to get the amount of time to nuke right, as microwaves vary in power.
start at about 4 minutes, then work up to longer times if they still contam after nuking.
a glass of water placed in the 'wave at the same time helps prevent the substrate from drying out.
|By jared (Jared112) on Monday, December 10, 2001 - 02:32 pm:|
I seen these 1cup bowls at kmart the other day for under $2 each and I think that they would look like the perfect thing to use for cakes. They are Martha Stewart brand(whoever the fuck that is!?) and instead of screwing on like jars, the lids snap on. They appear to be made out of a hard rubber, it says they are oven safe, microwave safe, and dishwasher safe, so do you think that they would be "steaming safe" or "pressure cooker safe"? I'm just worried about the lids melting or something like that.
The one on the very top is the 1/2 pint bowl. I think that if I used these I could just stick the syringe through the lid and it would be ok. What do you guys think about using something like this?
|By quote: (Quote) on Monday, December 10, 2001 - 03:14 pm:|
i've used similar containers with success b4.
|By jared (Jared112) on Monday, December 10, 2001 - 03:16 pm:|
So the lids won't melt in a pressure cooker or during steaming?
|By Nan (Nanook) on Monday, December 10, 2001 - 07:11 pm:|
Don't let the plastic touch the metal sides of the pressure cooker. I have heard reports of the lids deforming slightly if they come into contact with the hot metal.
|By Cragith Kilbonith (Kilborn) on Monday, December 10, 2001 - 09:39 pm:|
quote have you grown invitro with thoes rubbermaids? damn container touching the ground looks like a flat cake itself! would they melt if they are touching the bottom of the pc?
|By Kaijan (Kaijan) on Monday, December 10, 2001 - 09:45 pm:|
use a standoff..
Microwave Sterilization : Shroom Glossary