Honey tek / Mycelia growing in homemade syringes

Nan's Nook : Archives : Honey/Dextrose Q&A: Questions??? : More Liquid Culture Discussion : Honey tek / Mycelia growing in homemade syringes
  Subtopic Posts Updated Creator

By Dr. Cubesis III (Newbieshroomer) on Wednesday, November 28, 2001 - 09:59 pm:

Ok, first a few questions about the honey tek...

I have tried it once, with so far mixed results,

and I have started it again 3 days ago.

I took three wishbone salad dressing jars ( like ranch italian ect )
with the fat bottoms and narrow, long necks and pressure cooked
them at 15 psi for 45 min. The i filled them with distilled water
and added different amounts of organic raw honey to each one, the smallest amount a teaspoon, the largest a tablespoon.

I shook them really good, put tape ( clear packing tape ) over the single innoculation hole and replaced the foil. Proceeded to sterilize again in the cooker for another 30 min. After they cooled for a day, I then and brought out my syringe ( all syringes I use are put together in my hepa box, no contams yet, with over 15 syringes produced to date, innoculation is done in a positive pressure box or oven, depends on what I feel like using at the time. )
and innoculated about 2 cc's of fairly dark spore juice into each one, a little more actually, used a whole syringe between the three of them.

Now, the jars range in color from very light, to pretty dark colors right now.
the mycelia is growing at a rapid pace and you can see the black spores in the middle of large mycelia clumps.

My question:

1. I screwed up, the mycelia clumps I want to suck up with the needle
are at the bottom, if I shake the jar they are all over, is this ok?
Or should I figure out a way to just draw from the clumps when they settle?
Problem being, the needle barely reaches the top of the water in the jar......
What do i do??? HELP!!!

Uhm, actually thats it. Growth is flying, just not sure how to get the
damn stuff out of the jar... Should I turn the jar upside down and pierce the tape??? If the stuff leaks out, is there a chance it might act as a wick to infect the whole jar???

Sterility is not as important as i understand the hows and whys of my, er, our little hobby, but some things i still need to know, and learn.

Second question.

I innoculate ALL my own syringes these days, it's fun, it's easy, and I enjoy doing it. save myself TONS of dough by doing it by my lonesome. So far, ALL of my needles, have been packed in a fairly cool dark place and seem ok. They are very dark, spores aren't clumping and when I shake the syringe, it goes almost black. but ONE of them, one I was preparing to use last night, looks like it has mycelia clumping the spores together, LOTS of it. At first I thought it was contam, but upon closer inspection I realized I had seen the same growth in my honey tek water, virtually the same anyway. Fluffy, whitish, and clumped around the spores, Is it possible that I didn't properly clean a syringe i used on my previous honey teks? Might it still have had honey particles in it for the mycelia to feed on??? Or is it more likey a contam??? I am not sure whether to use it or not, but as my innoculated jar collection is rapidly approaching 90 I get less and less worried about losing a few. So far I have lost nine from my first batch, two to my previous failed honey tek, and one more to green mold,
and, with the contams cut out, all of these cakes seem to be recovering nicely cased in iceberg lettuce tupperware ( round tupperware containers.

Anyway, my boss just asked if I was writing war and peace, so I better get outta here, Any suggestions will be highly appreciated as I have 8 more pints that put together last night & sterilized this morning that
I wanna innoculate via my honey water this evening.

Take care all!!!
Happy Shroomin

The Newbie Shroomer

By Patrick (Valence) on Thursday, November 29, 2001 - 05:26 am:

First question.....Shaking it is fine as long as there is enough of a clump that when you shake it up the water becomes saturated with the white fibers over it. If not..wait a day or two for it to clump back up and remove some of the water so that it will be more concentrated...How to do this with your bottles...You could use Nan's needle making tek and make longer needles. Or you could pour it into another container using you flow hood but that is opening everything to contams.....

Second Question...You could just inoc a few jars just to make sure...I have a syringe exactly like that right now but I haven't used it yet.... Test it so you can tell me..

By Nugdumper (Nugdumper) on Thursday, November 29, 2001 - 05:30 am:

Question 1: I started two honey/water cultures 3 days ago, mine are in 1/2 pint jars, but we are seeing the same kind of growth right now. You need to wait on the cultures, they will actually form a very visible cloud of mycelium in the liquid. The stuff will be thick enough to even block your syringe sometimes. There will be no need to tip the jar, just suck the stuff up, you can stir it up with a sterile needle to break it some.

Question 2: I've heard it is actually possible for spores to germinate in a totally sterile syringe of water. I don't think it is too rare.

By Dr. Cubesis III (Newbieshroomer) on Thursday, November 29, 2001 - 07:34 pm:

LOL!!! Thanks you three!!

To Patrick, thanks for the needle making tek!!
I need longer needles, top to bottom the jar is prolly close to 8 or 9 inches ( if not taller )

I Halved the jars I had ready last night, 4 pints got new orleans blues ( whatever that is!! ) that I procured from Captain Max Mushroom, and the other four got the syringe that was experiencing mycelial growth inside. That syringe was one of the last that I had from Ralphsters free spore ring. A print marked COMBO that was almost as big as my hand. I know the print is not contaminated as I have been very careful with it and made at least 7 syringes from so far, over 20 jar innoculations with it to date and no contams so far .... Will Keep ya update Patrick, But i think nugdumper is right. It looks like clean growth, just not sure how it got there!

To Nugdumper. yea, I noticed when I sheake the jars they become very dispersed throughout the
glass, but I wasn't sure if I just want the clumps
or if shaking would be enough. At this point I think I will drain some of the water out in my big hepa glove box so I can go straight for the huge clumps at the bottom! Thanks Nugdumper, and thanks for setting me straight on the poll thing. Like Mr. Jared, I let me Newfound newbie enthusiasm get the best of me sometimes..... LOL! Though not as often as Jared!!!

Thanks guys!!! Will be sterilizing another 8 or ten jars tonight, innoculating via honey tommorrow.. Of course I will keep everyone posted :)

By Karna (Karna) on Thursday, November 29, 2001 - 11:30 pm:

The color of your jars is better lighter than darker.
Switch to half pint or pint jars. It makes no sense to culture in a container you cannot reach the surface of. This will eliminate the labor you are imposing on yourself to make elongated syringes. If you cannot get jars for some reason, check out glass food containers - a small spaghetti sauce or pickle jar, a medium to large jar of capers, a medium to large jar of marinated artichoke hearts will all work.
If you can actually SEE spores in your honey jar, you have used WAY too much spore solution.
Store all your syringes (the ones that have culture solution or spore water) in the fridge.
Everything else is cool.

By Dr. Cubesis III (Newbieshroomer) on Friday, November 30, 2001 - 01:48 am:

Thanks Karna,

Noted and VERY appreciated!