Invitro Speed Record

Nan's Nook: Archives: Spores: Strains: Invitro and shroom strain: Invitro Speed Record
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By quote: (Quote) on Tuesday, November 27, 2001 - 07:54 pm: The Nook

just had to brag.
12 out of 24 standard pf jars now have pinning visible only 19 days after inoculation.
strain pf classic.

By Lichen (Lichen) on Tuesday, November 27, 2001 - 08:02 pm: The Nook

that's pretty fast...and gives me an oppertunity to adress my critics from the shroomery. "And he probably has colonized cakes in 9 days, too"

No, actually I have never seen a fully colonized jar in under 12 days, so sorry

By quote: (Quote) on Tuesday, November 27, 2001 - 08:05 pm: The Nook

you can do it in 9, with plenty of liquid inoculant.
shaves off the 4-5 days needed to germinate.

By Lichen (Lichen) on Tuesday, November 27, 2001 - 08:09 pm: The Nook

well, I still can't perfect that tek, although that isn't to say I'm not trying

By jared (Jared112) on Tuesday, November 27, 2001 - 08:12 pm: The Nook

Ok Quote that's good but I'm working on a tech that will get pinning faster than that. I bet that I can beat you, however I guess that it will be considered cheating because I'm planning on using 1/4 pint jars.
Tomorrow I am also going to incolate 9 jars with 3 different Substrate formulas that I think will better emulate nature than PF TEK. 3 jars for each substrate mix, That should give me enough data to determine which one works the best. Wish me luck!

By quote: (Quote) on Tuesday, November 27, 2001 - 08:17 pm: The Nook

good luck,
you're going to need it.
but really, 3 jars won't tell you what works best everytime,
it could be just genetics or some other [unknown] factor which lets 1 set do better than the others.
you have to get the same results a few more times, it's called replicating the results.
if it's not the same every time for everybody,
then it's not proof.

By Lichen (Lichen) on Tuesday, November 27, 2001 - 08:18 pm: The Nook

If you're cocky enough, you don't need luck...look at Mohammed Ali

By nuecrew (Nue) on Tuesday, November 27, 2001 - 08:24 pm: The Nook

Muscle masters brothers, self mastery is bone- LaoTzu

By Great Expectations (Shade) on Tuesday, November 27, 2001 - 08:44 pm: The Nook

how the fizzy did you get your jars to pin 19 days after inoculation!!??!! I bow to your superior ways

By quote: (Quote) on Tuesday, November 27, 2001 - 09:10 pm: The Nook

simple, i give them light from day 1.
they were inoculated, pf max fruit formula, a little less than 1 cc of spores per jar [i get 12 jars per 10cc syringe].
they spent the first week huddled around a gallon jug of heated [aquarium heater] water,
then the second week on a shelf near a light bulb,
then the rest on metal shelves in a significantly cooler area.

By Snoopy (Snoopy) on Tuesday, November 27, 2001 - 09:38 pm: The Nook

Yep I believe quote could do it without problem I had 10 jars of EQ's that I innoculated on the 30th of october and when i birthed them on the 22nd a few of the jars had shrooms growing invitro that were about a centimeter in length... but that was 23 days..... but I Imagine if I had birthed them around 20 days I may have seen a pin or to.
I also had them exposed to light from day one....
they don't appear to be fruiting that great in the chamber tho..

By Dr. Cubesis III (Newbieshroomer) on Tuesday, November 27, 2001 - 09:54 pm: The Nook


I just dropped a big 11.60 on 12 more pint jars,
and was lucky enough to find a fish tank heater
at the swap meet this weekend... Looks like it's time to by a giant pickle jar.....

My point?? Lol! Right now my incubation for my entire stock is done soley in a cabinet stuffed with boxes... and a single night light up top. But I think now I will try quotes colonization method he has here with my newest jars, what a great Idea~! I really wanted to do some fancy incubation chambers but the boxes of jars are piling up and they'll eventually incubate anyway...

By Patrick (Valence) on Wednesday, November 28, 2001 - 01:55 am: The Nook

As a little experiment I sucked up the last bit of my Karo jar into a syringe and had only 2 jars left. So I inoculated the last two each with half the syringe. 5 cc's in each Jars. I did it slowly to let the jar absorb some of it before it drenched it. It actually didn't leave any visable moisture left. It was fairly thick with mycellia. Started out with 1 little pin of white on the 3rd day and by day five it was the size of a 50 cent piece. That was 2 days ago. It is growing at the normal rate now but it has a good head start. But as I just checked it, it is showing signs of moisture. When it gets to bad, I will flip it. And if it fails. Oh well. Always time for a new batch.

By jim brown (Shrhobbyist) on Wednesday, November 28, 2001 - 08:44 am: The Nook

I did four jars recently to test some mycellium water I had made. One got contaminated and the other 3 were 100% on the 13th day. They are producing great right now. That is my record by far. Also, Jared, I wouldn't make any bets yet it is going to be hard to beat the PF formula without a hell of a lot of experience. Good luck, though. I'd like to hear how it goes.

By wycrobro (Newman) on Wednesday, November 28, 2001 - 03:47 pm: The Nook

Two words LIPA YAI fully colonized 3 full pint jars in 9 days, from dry spores.came through the casing in 2 days. Just waiting for pins.

By ion ewe (Ion) on Thursday, November 29, 2001 - 09:07 am: The Nook

Oh yeah? Try 2 days! Granted, my buddy used myc water... and the substrate was all rice... and the jar was one of those little 1/2 ounce jelly jars you get in a holiday sampler...
But it only took 2 days! Super Micro Tek! We just popped 'em out and ate the things... not too shabby for a special holiday chocolate, either
Gift ideas folks?


Growing Invitro