Glovebox question!!

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I have glovebox questions!!! 4 6/03 glasshoppa
Operation of a Positive Pressure Glovebox     cerberus
Pros Debate: Oven Tek vs Glove Box  -    

By Festerfreak (Festerfreak) on Wednesday, November 21, 2001 - 06:37 pm:

I have a glovebox made from a 20 gl. fish tank and I was thinking of putting a negative ion generater in it.
It's about the size of two halfpint wide mouth jars and is suppose to cover 1500',is this a good idea or not.

By quote: (Quote) on Wednesday, November 21, 2001 - 07:02 pm:

not in the box itself, but outside in your work area would be good.

Be careful with flammable disinfectants and flame in a glovebox, they can (and do) explode! Sanitizer fumes are also highly toxic to mycelia

By Festerfreak (Festerfreak) on Wednesday, November 28, 2001 - 04:48 pm:

Sorry to take so long to get back to this quote but could you please elaborate on why I shouldn't put the negative ion generater inside the box. the box is totally sealed if that will help with any answer you may have.
Long live the fungi!!!

By quote: (Quote) on Wednesday, November 28, 2001 - 05:00 pm:

negative ion generators work by neutralizing the static electrical charge of dust particles, etc.
that makes said particles fall out of the air onto the ground.
that means there will be a really nasty residue on the floor near the generator,
which is not something you'd want inside the 'box, since your movements will easily swirl it back into the air.

By prepschoolquote (Quoschoolprepy) on Friday, December 07, 2001 - 08:05 pm:

C:\My Documents\My Pictures
C:My DocumentsMy Pictures91295069129506

By Cbee (Cbee) on Saturday, December 08, 2001 - 01:05 am:

thats what i need

By Cbee (Cbee) on Monday, December 24, 2001 - 12:44 am:

Well i finally made myself a glovebox today! in 3-4 days i am goina try it out and make some prints, after i check if i can make em sterile i will post in spore trade to see if anyone would like to trade some prints Anyways just wanted to tell the world I finally got around to doin it, used a fish tank and plywood w/ dishwasher gloves! put a nice weatherstripping seal around the edges too! woohoo! maybe ill get some pics sooner or later, as soon as I get a damn USB card for my comp!

By Cbee (Cbee) on Monday, December 24, 2001 - 01:52 am:

hmm ok, i have been reading for then last while about glovebox use, etc. I still dont get it tho. Do I spray the inside with lysol then put all the stuff in it i am goin to use? If i do that then wont it kill the spores? I am wanting to make prints right now so I know i wipe the cards/tin foil off with alchohol then let them dry, place in glove box, cover the clean portion, spray the box with lysol, but how do i have the cap inside while i sterilize?? i dont get it! any help or links that i cannot find would be great i have been searchin the archives for info

By quote: (Quote) on Monday, December 24, 2001 - 03:51 am:

you put all the stuff in 1st, including the lysol, then you spray lysol after it's sealed.
you can put the cap in a jar, or wrap items in foil, to protect them from direct lysol contact.
never light a flame in a lysol-filled box.

By Liberty_Caps (Liberty_Caps) on Monday, December 24, 2001 - 06:33 am:

wont the lysol fumes kill myceilum and spores???
i'm makeing a glove box tonight thanx

By Nan (Nanook) on Monday, December 24, 2001 - 08:24 am:

Concentrated Lysol fumes will kill the gills of printing caps. Pretty quickly too. If you are using a sealed box and you are going to be printing... Probably a good idea to use 10% bleach solution and spray with that instead of Lysol.

By UnkyHerb (Notapplicable) on Monday, December 24, 2001 - 12:48 pm:

Everyones getting into the glovebox buisness :) I just built one usuing a dust mask, rubbermaid, and a muffin fan with a power source.. It beets the hell outta a 300 degree oven ;)

By Bob Smith (Maliki) on Monday, December 24, 2001 - 02:05 pm:

Glove boxes are beatiful things. I love mine , mine was made from a big rubbermaid container, onle large side of a ten gallon aquirium for the see thru top cut into the plastic top and sealed with RTV sealent , for the gloves i just used Subway sandwich bags taped all around the edge of the wholes with ducttape. The end cut off of the bag with a rubberband rolled into the end of the bag inside the box. And latex exame gloves, so what happens here is the gloves are on, all materials in box ready to go , I slide hands into holes thru bag where the rubberband closes snug around my wrist. Spray the insides with bleach solution let stand for a few minutes and start work its all good.......Blessed Be

By Dr. Cubesis III (Newbieshroomer) on Monday, December 24, 2001 - 06:18 pm:

Positive pressure hepa box, open holes, no gloves...

Nary a problem yet :)

By Cbee (Cbee) on Monday, December 24, 2001 - 06:18 pm:

i bought lysol for nothin nan? Alright, well Im goina be makin up some syringes from some prints i got from ralph today. Figure if i wipe down a shot glass with alchohol, toss it in the glovebox, with the print and a few syringes full of sterile water (boiled syringes while dissasembled and then assembled with clean tongs and alchohol cleaned hands, and sucked in and squirted out 3-4 times with boiling water, then sucked in once, put the caps on the needles and tossed in a zip lock) oh ya and use a sterile scapel or something of the sort to scrap the spores off, should work, right??

By Nan (Nanook) on Monday, December 24, 2001 - 06:46 pm:

Sounds like a plan Cbee You don't need to disassemble the syringe plunger from the barrel.

By Cbee (Cbee) on Monday, December 24, 2001 - 07:26 pm:

thanks nan, the stuffs been sitting in the glovebox for about 30 mins since i sprayed it. Goin down to make some syringes =) wish me luck!

By Cbee (Cbee) on Monday, December 24, 2001 - 07:48 pm:

wow, that wasnt too bad! i heard i have to wait 24hrs till i knock up some jars, is that true? i wanna test a syringe in some honey water to see if its contamed, is that possible to do right now? I got 6 beautiful Plantasia Mystery syringes, with 1/4 of the print left!! woohoo! that wasnt so bad

By Nan (Nanook) on Monday, December 24, 2001 - 08:01 pm:

You can inoculate liquid culture directly with dry spores, no need to wait as the liquid media will rehydrate spores just fine. In fact I inoculate liquid culture jars directly from prints and skip making spore syringes altogether.

I would think you want to wait 6-12 hours minimum rehydration time for spores before you shoot PF jars.

By Cbee (Cbee) on Monday, December 24, 2001 - 08:04 pm:

ok cool nan, im goina go shoot up a honey jar =)

By Cbee (Cbee) on Monday, December 24, 2001 - 08:30 pm:

k, i gotta tell you guys, dont microwave a 1/2 pint with a plastic lid, i picked it up outa the microwave and crash the lid fell off, honey water everywhere! i dunno why it was fucked. Anyways i heated up some water, thru some honey in it (in a 1/2 pint) thru a lid on it and into the steamer it goes. about 30 mins should do I think. i dont need a PC to do this do i? arg i hate not having one, shoulda asked for one for xmas =)

By Nan (Nanook) on Monday, December 24, 2001 - 09:28 pm:

I have no posted Tek on steamer sterilized Honey Tek... But if it works for jars...

If you get good results, post it as Tek for the next guy...


By Cbee (Cbee) on Monday, December 24, 2001 - 11:19 pm:

will do, i just shot it up, so ill let ya know what happens in the next few days in this post, but then again if it contams it could be 1)my syringe or 2) the unsterile jar... we'll have to see what happens

By Kman (Kman) on Friday, December 28, 2001 - 07:08 pm:

CBEE? how did that honey water turn out? Since I got 2 of those syringes of you I sure am curious to know if it was contaminated or not? I gonna go shoot some honey water myself later!

By Cbee (Cbee) on Saturday, December 29, 2001 - 12:30 am:

the steam sterilizing of those jars didnt work, I guess you gotta PC them.

By Hatcher (Hatcher) on Saturday, December 29, 2001 - 08:55 am:

Thirty bucks for a six qt. aluminum pc is a small investment I think.

By Scotsman (Barrowland) on Saturday, December 29, 2001 - 12:14 pm:

What if i cant find lysol will any spray you use on cuts be ok.

By quote: (Quote) on Saturday, December 29, 2001 - 03:49 pm:

you can substitute chlorine bleach, used for laundry, instead of lysol.
dilute it 10:1 water:bleach.