Jared's Incubator

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By jared (Jared112) on Monday, November 19, 2001 - 09:15 pm:

I am growing PF 'shrooms everything PF TEK and I want to know that since I just have the jars sitting in the incubatore like this...
Will this cause premature pinning before the cakes is fully covered with mycelium? or am I fine? If not I suppose that I can put them back into the box and put the box into the incubatore, but that would probably be a bad fire hazard. Is it ok or should I cover these little bitches up asap?

By Underground_Shaman (Shaman) on Monday, November 19, 2001 - 09:30 pm:

With the full pint jars, you could see some pinning before the cake is fully colonized. You could just wrap the glass in foil and it should prevent that.

Also, keep the lid holes covered. This should help.
Remember, too, that you need Oxygen to induce pinning. That's why the tape is removed at 50% colonization during incubation...to induce pinning invitro.

The scientist in me wants to do half and half, just to see if it does pin invitro before full colonization. But practically speaking, cover the jars with foil and prevent air exchange in the jar. This should give you healthy mycelial growth without premature pinning.

Hope this helps.


By An guy (Boomer) on Tuesday, November 20, 2001 - 02:02 am:

::Raising hand::

Yuh- you can get pinning before complete colonization, I can attest to that....

But, it's not the end of the world, or ain't so far on my cake- I'll let you know what happens.

Exactly like Shaman said- I was getting pinning, had taken off the tape and flipped the jar. Cake wasn't fully colonized- one or two little pins.

But it was *moist* in there, and on the bigger pin, I could see the brown/brown striping that indicates more-than-needed moisture levels, so I unscrewed the jar from the cap, without taking the jar off the cap- just to get a little more airflow- started throwing pins right and left, and the cake wasn't fully done.

I birthed it last night, put some dampened verm in the microwave to sterilize it, and cased the top of the cake, where it wasn't colonized. Where it isn't colonized, it's more susceptible to contamination, which is why I wanted that spot covered, and why I wanted to mic both the verm and water that went on it. Since then I've dampened it with raw water, on the principle that it isn't going to mkae it clear down to that vulnerabvle spot, which by now might be colonized or not, I dont' know, but the upper particles of verm I'm thinking wil....anyways, I'll let you know what happens in case you're ever faced with the same thing...

boomer the talker

By John Sparks (Sparks) on Saturday, December 08, 2001 - 07:47 pm:

I am fairly new to this growing and have yet to produce anything impressive. (Currently looking at 3 cakes with only 1 pinhead on each) but back to the question. I am planning on incubating my jars in a warm bath in my aquarium. I can maintain the temparture with a heater and keep the water temparature at any temp. Is there any tips, precautions or recommendations for this setup? I plan on only sitting them in water half way up the jars. Should not worry about this and just sit them in my closet, Thanks Sparks

***Happiness is an inside Job***

By Nan (Nanook) on Saturday, December 08, 2001 - 08:42 pm:

Water bath incubators are fine and keep the humidity up nice and high. It's a proven Tek. Use a strong salt solution (very messy) or periodicaly add a few drops of tincture of iodine to the water to control contams.

Dry type incubators can cause excessive drying in jars especially in cooler weather when the humdity and temps are low. People screw the lids on tight and cover the holes with non-porus tape (electrical type tape) to conserve the jar mositure.

By jared (Jared112) on Saturday, November 24, 2001 - 06:31 pm:

Ok my cakes are all around 50% colonized here is a pic of one of them.

Now I am going to turn on my oven and make that a sterile work place, so what do I want to do? Just loosen the lids of the jars a bit and leave them like that? turn them upside down and leave them like that? or just take the tape off the top of them and shake them? somebody tell me what I should do and how I should do it so that I can speed it up. I can't believe this! I have only been incubating these jars for 6 days and I already have this! I'm telling you people 85 degrees F, is the sweet spot!

By Lichen (Lichen) on Saturday, November 24, 2001 - 06:35 pm:

you don't need to do anything to the lids or the tape if it's been only 6 days. Just leave them alone, they're doing fine. Don't shake them or open the lids. You can take the tape off, but you really don't need to yet.

By jared (Jared112) on Saturday, November 24, 2001 - 06:54 pm:

well will you tell me what I need to do when the time comes? or at least tell me how I know its time and exactly what I should do?

By quote: (Quote) on Saturday, November 24, 2001 - 07:05 pm:

you're fine.
just let them grow.
if they stop growing, remove the tape.
otherwise, just let them be.

By jared (Jared112) on Saturday, November 24, 2001 - 09:46 pm:

So how long do you guys think it will take before I have to birth the jars? another full week maybe? How long after full colonization until I start to see some pins, these are PF and they are incubated at 85 degrees.

By Liberty_Caps (Liberty_Caps) on Saturday, November 24, 2001 - 10:51 pm:

I'm my experinice I say just birth a week after 100% colinization.
but with the PF race u might want to wait till u have pins in the jar then birth.
good luck

By Lichen (Lichen) on Saturday, November 24, 2001 - 11:43 pm:

you should see pins a week after full colonization. Fanaticus puts it at 23 days, and that's usually right on the money. However, it's not necessary to wait till it pins; recommended, but not necessary.

By greenthumb (Greenthumb) on Sunday, November 25, 2001 - 12:39 am:

invitro pins

By Stewie (Stew) on Tuesday, November 27, 2001 - 05:40 pm:

This is my first time growing without help. I have 24 jars and planning to grow invitro.My jars are at about 15-20% colonization one week after I innoculated. I want to speed up colonization but i have no incubator so I placed the jars in their original boxes on top of the refrigerator. I am hoping this will provide enough heat to speed things up a bit, but I don't want to overheat or dry out the jars. Has anyone tried this or have any suggestions? Thanx all.

By quote: (Quote) on Tuesday, November 27, 2001 - 05:58 pm:

sounds ok to me.
another good place is a closet, if you can get a shelf fairly near a light bulb, it will heat things up nicely.

By jared (Jared112) on Tuesday, December 04, 2001 - 04:38 pm:



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