|By Admin (Admin) on Thursday, August 23, 2001 - 12:18 pm:|
Honeywater mycelium Tek
Honeywater mycelium Tek
A fast way to colonize your jars
The achievement of this tek is to shorten the time needed to colonize a jar as you'll see signs of growth in 24/36 hours, instead waiting 3/5 days for spore germination.
What you need:
A narrow mouth jar with lid
Organic honey - best is the pale yellow one
Some H2O2 (at 3% or 12 vol.)
Some heat resistant silicone
A filter disk
How to do it:
Drill a small hole in the lid, large enough to pass through with a needle.
Mix a small quantity of honey (less than a teaspoon) in the water, the water must be very light yellow colored.
Sterilize for 30 minutes and let it cool (don't over sterilize or the sugars in the honey caramelize).
Inject a small quantity of spore solution, 1-2 cc it's enough.
Put in a dark warm place, better to stir a bit once a day, and wait until you see small white hairs floating in the solution,
wait a day or 2 then add some H2O2 , few cc's, (I would put 2 cc in a 1/2 pint jar) then put the jar in the fridge. This helps to keep out contaminants. Specially bacteria.
Now the honeywater it's ready. To use it simply suck some water with a sterile syringe and inoculate your MMGG/PF TEK style jars as usual.
The solution will last at least 2 weeks, but anyway i wouldn't use a solution much older as the risks of contamination increases.
Some optional improvements:
A drop of silicone closing the hole helps to keep out contaminants.
advice!! If you close air tight your jars the sterilization will produce a negative pressure that will suck ALL your syringe into the jar. Best to tight the lids after sterilization.
You can drill 2 holes in the lid and use one to inject spores/suck liquid mycelium and the other one for gas exchange as shown in the the pic
|By Nugdumper (Nugdumper) on Monday, December 03, 2001 - 06:33 am:|
Alright, so I have two honey/water cultures in half-pint jars, using Quo's Microwave Tek. They are 7 days old today. What sort of growth should I be seeing right now?
There is definitely mycelium in the bottom of the jar, but it isn't forming a cloud yet, is this a sign of a prob? Also, when I look from underneath the jar, it looks like there is some sort of clear film in the very bottom center of the jar. Is this the honey breaking down or a sign of bacteria? ANY help is mighty appreciated people.
|By Nan (Nanook) on Monday, December 03, 2001 - 07:31 am:|
Read about cloudy scummy honey tek in my post on Karo Tek.
It will still work fine. Honey Tek generates a lot of questions because of the impurities.
|By Nugdumper (Nugdumper) on Monday, December 03, 2001 - 09:00 am:|
I can definately tell that the spores have germinated, there are several large spore clumps to see with the naked eye. So far I don't see discoloration or anything, so when should I be worried I have contams? What signs to look for?
|By quote: (Quote) on Monday, December 03, 2001 - 11:18 am:|
one of the biggest drawbacks of liquid cultures is the difficulty in spotting contams with the eye. many times i've had batches which looked perfectly good, yet turned out to be infected. my advice is to always test home-made syringes on a few jars before mass inoculations.
|By Nan (Nanook) on Monday, December 03, 2001 - 11:35 am:|
One of the biggest advantages of a crystal clear culture is that Contams are quickly and easily caught by the Mark IV Eyeball once the eye is trained glassing a jar or two.
|By Nugdumper (Nugdumper) on Monday, December 03, 2001 - 08:01 pm:|
The mycelium should form a cloud correct? If this does not happen within 14 days then there is contamination correct?
|By quote: (Quote) on Monday, December 03, 2001 - 09:58 pm:|
generally you'll see individual clumps in 5-7 days, which then join to become a cloud, filling most of the liquid.
after 14 days w/o visible germination, it'd be safe to assume failure.
|By Nugdumper (Nugdumper) on Monday, December 10, 2001 - 06:19 am:|
Ok, 14 days after starting my they both have small clouds forming. Should they be further along at this point? And is there any way to assume failure at this point? There is also alot of stuff on the bottom of the jar, probably from the honey, but it is white, and I'm not sure if it is good or not. I got the syringe from Ryche, and it had huge purple clumps in it. These have visibly germinated, it can be seen easily with the naked eye.
|By Nugdumper (Nugdumper) on Monday, December 10, 2001 - 06:22 am:|
Ok, being high, I just made that last post, not realizing I totally repeated myself in every line. My apologies for the waste of space. I should smoke less.
|By Nan (Nanook) on Monday, December 10, 2001 - 08:06 am:|
If the jars were incubated and have run for 14 days at temps of at least 80*F since spore inoculation... I would go ahead and open the jar in the Glovebox, thrash the mycelia with the needle, maybe draw it up and squirt it back out into the jar to fragment the "puffs" of mycelial growth...
And Shoot It.
|By Nugdumper (Nugdumper) on Monday, December 10, 2001 - 08:45 am:|
Cool, I'll try it.
|By quote: (Quote) on Monday, December 10, 2001 - 05:08 pm:|
test it on a couple jars before you do a bunch of them.
|By Nugdumper (Nugdumper) on Tuesday, December 11, 2001 - 10:08 pm:|
Both jars looking good now, visible cloud in the liquid, looking like thick mycelium. I will be shooting both of these on Sunday, we'll see if they are any good.
|By quote: (Quote) on Wednesday, December 12, 2001 - 04:20 pm:|