|Karo tek stalled?||6||12/22 02:57am||onediadem|
|By onediadem (Onediadem) on Wednesday, November 14, 2001 - 04:26 pm:|
The jars have about the size of a quarter, of a white stringy fuzzy looking mass in them. I did two jars. 1 jar has a black dot in the middle of this mass, and has not grown or done anything, so maybe it ws something in the jar. My q' is this. Its been 3 days, The temp is set at @ 82-84 degrees. Seems like the growth has slowed. How long should I wait to draw up in syringes? thx folks.
|By quote: (Quote) on Wednesday, November 14, 2001 - 04:53 pm:|
you can do it now if needed.
but i'd pass on the one with the black spot.
|By Nan (Nanook) on Wednesday, November 14, 2001 - 05:25 pm:|
Pam you can shoot anytime between now and day 7. I usually pop them in the fridge after a week if everything has gone well. With fresh spores and incubated liquid culture jars you can indeed see spore germination in 24 hours and be ready to shoot in 3 days.
Be sure to break the clump up well once the jar is opened in the glovebox. Then you will be good to go for the Western Show
And Quo's right. The jar with the black floatie is probably good, a bit of dirt in the jar before sterilizing, but if you have a choice use the cleaner inoculum. You can always test a few jars first before running an entire batch from that inoculum with the floater.
Lichen... Any word on your Karo jars shot with spores?
|By onediadem (Onediadem) on Wednesday, November 14, 2001 - 06:38 pm:|
Okee, think I will shoot em tommorrow.I am gonna let the black floatie one go 7-8 days and see if anything comes of the dot. The mycel in that jar is doing well. But, it does have the dot. thanks guys
|By Snoopy (Snoopy) on Thursday, January 31, 2002 - 04:21 am:|
Hey everyone remember me?
I was reading Nan's Karo Tek post in the archives and it sounds pretty cool, and wanted to perhaps give it a shot.
Nan do you think, after you have tried this I assume many times, that it is the most efficent way of colonizing jars? It seems like you could produce more jars with less spores. But spores aren't an issue for me as I have many prints. What say you nanook of the north.
|By onediadem (Onediadem) on Thursday, January 31, 2002 - 05:49 am:|
Hey Snoopy, I started off with karo tek, never did a jar with a spore syringe. Nan is a Genius.
Glad to see ya!!
|By Nan (Nanook) on Thursday, January 31, 2002 - 03:25 pm:|
Yes, overall Karo tek is faster than spores. The reason? The spores are all germinated in the Karo, you shoot jars with live growing mycelia and the jars show growth in 24-36 hours. Shooting spores into jars you are going to wait 3-7 days to see the same progress.