|Simple Cubie Tek||-|
|By jared (Jared112) on Friday, November 09, 2001 - 04:49 pm:|
I was just reading about how people say that steamig is not a good way to sterilize substrate jars. I don't have a pressure cooker or know anybody with one, so I'm going to have to steam my jars. Is there a way to make sure that I can do it perfectly or is steaming just the shwag way of doing it? If somebody has a fool proof way of steaming please let me know.
|By XUnkyHerbX (Notapplicable) on Friday, November 09, 2001 - 04:52 pm:|
you can steam pf jars.. steaming grains/seed will leave you with contaminats down the road.
|By Timothy Leary (Timothyleary) on Friday, November 09, 2001 - 04:56 pm:|
i put them in a strainer(colonder) over a pot of boiling water, put a lid on top and let them sit
for 1hr 10min.
i have never had any contam issues doing it this way.
*keep your eye on the water, make sure it all
doesn't eveaporate. i know have it down to where
i can get the water to last 2hr20min b4 a refil,
practice makes perfect
|By jared (Jared112) on Friday, November 09, 2001 - 05:26 pm:|
Ok so you think that if I do it for a little more than an hour then I won't have any contams. I'm going to be using the PF Tek. So I should be fine then?
|By quote: (Quote) on Friday, November 09, 2001 - 05:49 pm:|
i used to say that steaming worked great,
but some bad experiences a few months back
left me less convinced.
steaming can work,
but it's a last-resort option.
you can expect to lose a few jars if you're forced to do it,
but most should be ok.
definitly steam a full hour, or even 15-30 more.
|By property of Friedrich Nietzsche (Economist) on Friday, November 09, 2001 - 05:59 pm:|
I did 10 jars with steam for an hour. two contam'ed, but that was due to questionably sterile circumstances during inocculation.
so go for it.
|By Timothy Leary (Timothyleary) on Friday, November 09, 2001 - 06:10 pm:|
yea, steam for more then an hour, you will be fine.
remember to keep the jars lids loose
and foil covers on.
|By Cosmic Charlie (Cosmiccharlie) on Friday, November 09, 2001 - 07:59 pm:|
Any of you heard of autoclave tape? I used it when I steamed my jars, it changes color when its reached sufficient temperatures for long enough time to kill contaminants. Its an indicator that you've been successful in autoclaving, used in research labs all the time. Just put a piece on the outside of the jar. Gives you a little peace of mind after steaming.
|By Timothy Leary (Timothyleary) on Friday, November 09, 2001 - 08:11 pm:|
here is a link to where u can buy some
|By greenthumb (Greenthumb) on Friday, November 09, 2001 - 08:12 pm:|
Fanaticus gives great instructions for boiling/steaming and pressure cooking PF jars. I love my PC. It only cost about $40.00 but I use it all the time!
Good luck and be sterile!
|By Nan (Nanook) on Friday, November 09, 2001 - 08:36 pm:|
Here is our current link to Pressure Cooker Suppliers. Ebay is a good bet, good used cookers are 30%-50% off list (they hold their value guys, it's an investment). All the intermediate and advanced Teks here specify a Pressure Cooker. Look for the All American brand... They are gasketless.
|By relic (Relic) on Friday, November 09, 2001 - 10:29 pm:|
i steamed for over a year when i started and never lost a jar.
|By Happerous Maximus (Happyguy) on Friday, December 28, 2001 - 03:36 pm:|
I keep hearing one should use a P.C., but i ruined mine converting it into an alcohol still (minor catastophy) and it is gone. Sooo.... please help
1. How good is boiling water in a huge pot to sterilize?? and for how long etc??
2. Why can't I just use an oven?? And can I use an oven to sterilze sealed jars full of water, or perhaps water and dextrose/honey??
3. If I buy spring or distilled water at the store in plastic gallon jugs, is the water sterile, or does it harbor contams?? Hence, if I were so inclined could I innoculate the entire gallon jug via sticking a syringe with sterile dextrose and spores through the gallons own plastic cap????
4. Can I use an oven to sterilze anything, water, anything?? (i really want to use an oven.) jars of water?? honey??
5. If oven sterilizing anything is possible, what temp/time and for what?? im prolly sounding like an idiot but im curious.
Thanks muchos grandes bien.
|By quote: (Quote) on Friday, December 28, 2001 - 04:39 pm:|
pf tek, you can steam your jars on the stove.
but an oven is not suited for sterilizing substrate.
and bottled water is not sterile.
|By Happerous Maximus (Happyguy) on Friday, December 28, 2001 - 05:10 pm:|
thanks for the ino about the bottled water, i suspected as much. As for basic pf tek, a friend of a friend has indeed had a lot of success with it. However I am interested in other endeavors that would be 1000 times easier if i could use an oven to sterilze some of the components such as jars of water or agar solutions, or dextrose in water. Even basic tools. I really wish my friend didnt blow up my kitchen, i would still have two pressure cookers.
As for PF jars in a pot of boiling water, how long??
Thanks for this amazing site.
|By Serialkiller (Serialkiller) on Friday, December 28, 2001 - 06:15 pm:|
steam at a rolling boil for about an hour
|By onediadem (Onediadem) on Friday, December 28, 2001 - 08:24 pm:|
Check out thrift stores or flea markets for a used pc. When I wasnt into the myco hobby, i saw em sometimes for a couple bucks.
|By An guy (Boomer) on Friday, December 28, 2001 - 08:29 pm:|
I steam at a low boil for about 1/2 hr, maybe 45 minutes- that means the stuff is in the pot longer, letting the water get back up to the boil, and then shutting the burner off to cool down some before I take them out.
ovens are dry heat, and much harder to sterilize in- a good degree of higher heat is needed.