|Mycelial Syringe Project||-|
|By plinkerdink420 (Plinkerdink420) on Wednesday, November 07, 2001 - 11:23 pm: The Nook|
about three days ago i innoculated four jars with liquid from a liquid culture, and, as yet have no signs of growth in my jars... i repeatedly plunged the syringe up and down to stir up the water a bit and even lashed the needle around a bit inside the water... but maybe my needle is too thin... it is a regular needle like they use in a doctors office... sharp and thin.. comes on a 10cc syringe... don't know what else the problem could be
|By Nan (Nanook) on Thursday, November 08, 2001 - 02:22 am: The Nook|
I started using liquid cultures with the standard needle that PF ships on his syringes, and those work just fine as long as things are not too chunky. I have never used anything thinner... But there are ways to check if things are looking OK during the sterile part of the Western Show (Draw and shoot}
Did the needle clog or was the plunger very stiff when you were drawing up? That is not a good sign. After drawing up, glass the syringe barrel in front of a decent light... You should see hundreds of little "floaties" in the syringe. If they got in, they will shoot out just fine.
I assume the liquid culture grew out normally and you had clean, viable, mycelia to shoot with. Contams in liquid cultures (Yeast esp) will prevent the mycelia from "taking" to substrate.
Maybe things are just exceptionally slow. Jars are being incubated???
|By Lichen (Lichen) on Thursday, November 08, 2001 - 02:51 am: The Nook|
wait another 2 days.
|By plinkerdink420 (Plinkerdink420) on Thursday, November 08, 2001 - 02:57 am: The Nook|
yeah.. the jars are being incubated at about 90-92 degrees... i don't think any mycellium (sp?) got into the syringe... i looked into the syringe for the "floaties" but didn't see any.. my needle is much narrower than PF's... mine is from a doctor's office... i think i will just have to use up the rest of my spore solution so i can use the wider gague needle... thanx for the help!!
p.s. i loved the western show thing hee hee
|By Nan (Nanook) on Thursday, November 08, 2001 - 04:32 am: The Nook|
Knock off 4-5 degrees from the temp... Get some floaties into your syringe... You should see growth at the inoculation point in 24-36 hours.
|By plinkerdink420 (Plinkerdink420) on Thursday, November 08, 2001 - 02:01 pm: The Nook|
|By Ryan Waters (Zerogravity) on Wednesday, November 28, 2001 - 07:46 pm: The Nook|
I am using the liquid mycelium innoculation tek too (Still green though) and I went to the drug store and my ex girlfriend (a pharmacist) showed me several guages of needles (tips) ranging from 22 guage (standard tip, not too painful looking)to 16 guage (something I would NOT let a nurse come near me with) and with the same concerns you have, I chose 20 guage disposable tips (at 11 cents apiece) and a few 18 guages just in case I have to suck up a few chunks later on. Any thoughts on this Nan?
|By quote: (Quote) on Wednesday, November 28, 2001 - 09:05 pm: The Nook|
nan's on vacation,
i'm minding the store.
how long are your needles ?
long & wide works best.
|By nuecrew (Nue) on Wednesday, November 28, 2001 - 09:52 pm: The Nook|
A few years ago I had a needle used for spinal taps. It was 4 inches long and had a removeable wire that went the length of the needle for added strength when..??? After taking the inner wire out you had a rather large long stainless hypodermic needle with a luer-lock end on it. The pack it came in said,"Federal law prohibits dispension...." you know the rest.
|By plinkerdink420 (Plinkerdink420) on Wednesday, November 28, 2001 - 11:40 pm: The Nook|
nice... oh yeah, BTW... i used Nan's tek for a homemade needle from the link above and it works like a charm... also using a pf needle... everything is good now