|By relic (Relic) on Sunday, October 28, 2001 - 06:17 am:|
well i just put this thing together
18" x 24" piece of plexiglass
rubbermaid 44 qt. clear storage bin
(2) 4" male pvc adapters
1/8" thick foam tape
1/8" drill bit and drill
#7 x 1/2" sheetmetal screws (zip screws)
i had planned on finding some long gloves to use with it attached with large hose clamps but had no luck finding any so far. i will try the bait store next. for now i'll just use latex gloves with open holes.
to cut the holes in the sides i used the 1/8" drill bit and drilled holes close together around the lines i traced around the PVC fittings and connected the dots with the razor knife. then the pvc fittings were screwed in(don't make the holes too big!) i then saved the circles that came out and foam taped around them with notches in either side of the tape around the circle so it could be applied w/o ripples. i will use them as hole plugs to keep the box clean when not in use. i will prolly epoxy some sort of handles or knobs on these so they can be removed easily from the outside of the box.
the hole in the lid was easily cut with the razor knife. the plexiglass was scored with the knife then snaped to size. holes were drilled in the plexiglass then wottled out to be oversized so the plexi didn't crack when i put the screws in. the lid was a softer plastic and was not drilled. plexiglass was applied with foam tape and zip screws manually with a 1/4" nut driver. the holes have to be close, i.e. 3", together to ensure the foam tape sealed (silicone could be substituted here for foam tape)
check it out,
one of these really isn't essential for making syringes or even grain to grain transfers, but, after trying agar a few times without one, i decided i needed one.
Be careful with flammable disinfectants and flame in a glovebox, they can (and do) explode! Sanitizer fumes are also highly toxic to mycelia
|By Professor Fustertingle (Fustertingle) on Monday, October 29, 2001 - 10:52 pm:|
A person can get shoulder lenghth gloves at a farm supply store. They are used for checking to see if the cows have been bred or not....you city folk use your imagination....
|By Hatcher (Hatcher) on Tuesday, October 30, 2001 - 04:22 am:|
Relic, if you'd please.. I've always wondered... how do you get the work in and out of the glovebox and know the interior of the box is contam free? Do ya employ a clean-room too? Just curious! Thanks!
|By relic (Relic) on Tuesday, October 30, 2001 - 05:01 am:|
well, w/o having a HEPA hooked up to it that is virtually impossible. i'll spray it down good with lysol and start out clean. then, as far as i can tell, i'll only have to open the box once to pour the plates and once to put Culture/Print/Loop/Exacto/Lamp, etc. the only time any culture media will be exposed with the box open is when Agar plates are poured, that shouldn't be a problem and is kinda hard to avoid, right? i think i can manage to do that without Contamination, i get by with open jars of Sterile Grain for short periods during transfers in still air environment. The box will allow me to take my time when working with the plates without having to worry about my breath or dust or skin flakes, falling debris etc. or trying to hurry and fuck up. If you like working with agar plates over an open oven, God bless you. I'd considered building a Flow Hood for doing agar work but my wife would probably have my balls on the table if i did. where are we going to put it, what will we tell people it's for, etc. lol. and this was like $20-$25 in materials
Shroom Glossary : Lichen's Box : 9er Tek Box : Dr. Cubie's Box : Nanook's Box