|By Nuecrew (Nuecrew) on Wednesday, October 24, 2001 - 03:22 am: The Nook|
I bought a spore print from Spore Works. It came in a little 2X3" baggie. I cleaned the baggie with alcohol hand cleaner. I used all new syringes and needles. I PC'd a glass of water with two layers of foil over the top. I took the first layer of foil off the water glass. I then poked the needle through the second piece of foil left on the glass and into the water. I then filled my syringe with water. I injected the whole 10cc's into the little baggie and worked it a little with my fingers. I then sucked the deep purple water back up. I injected on the side of the baggie that allowed me access to the open end of the fold in the spore print. (so I could squirt the water around inside the print)I repeated this 6 times hitting the same hole in AL foil and baggie each time. I then innoculated 6 PF Tek style 1/2 pints with each of the 6 syringes to test for contamination. This was done last Thursday night and I don't see any contamination now, Tuesday night. I do see lots of white mycelia in four places around the jars. I was wondering about the air that replaces the water lost to the syringe. Will this cause contamination?
|By Nan (Nanook) on Wednesday, October 24, 2001 - 05:34 am: The Nook|
Not normally. - Printing in baggies
|By Nugdumper (Nugdumper) on Wednesday, October 24, 2001 - 06:35 am: The Nook|
I assume it was a 10cc syringe? It can make the substrate a little thick, depending on the verm grade mainly. That many spores and those jars should be done quick. Was it a decent sized print? I like your method, easy print to syringe technique.
|By Nuecrew (Nuecrew) on Wednesday, October 24, 2001 - 01:15 pm: The Nook|
Nugdumper you brought up a good point I didn't think of. Have you had problems or do you know of problems associated with too many spores per cc? I am going to take the syringes and dilute them at least 10:1. It will be a waste to use them all as is.
|By Nan (Nanook) on Wednesday, October 24, 2001 - 03:58 pm: The Nook|
Probably a good idea to dilute them from I have read: Too Many Spores?
|By Nuecrew (Nuecrew) on Thursday, October 25, 2001 - 01:52 am: The Nook|
Thanks for the input. It does seem like nature to make the best from the hardest situation. Man always spoils nature with too much of a good thing.
|By Nan (Nanook) on Thursday, October 25, 2001 - 03:07 am: The Nook|