Oven Door Sterile Tek

Nan's Nook : Archives : Gloveboxes : Oven Door Sterile Tek
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By Nan (Nanook) on Tuesday, October 23, 2001 - 07:00 pm:

Captain's Oven Technique

Here is a method I've been using for over 15 years. If you follow it correctly you can open/close jars, do innoculations etc all while staying sterile. First you need an expensive peice of equipment, an oven. Lucky for most we have already made that investment. If you don't have a oven, don't buy one, go get a HEPA filter instead. Now for thoes of you with ovens. Turn your oven on the lowest heat setting. Pull one of the racks out far enough that you can set stuff on it and also work on it. Place your jar/sporeprint/agar on the rack and open it, only after the oven has heated up and there is a steady flow of warm air rising. The steady flow of air will keep anything from dropping on the jars. As long as you use a breather, or don't breath close to the jars. Of course everything else needs to be sterile, knives/loops/hands so that you don't contaimate things that way. Basically this will stop all Airborne particles. Anything else needs to be clean. If you don't have an oven, or your racks don't pull out nicely, you can build a rack above a range and turn it on, same principle. I've tested this many times over, even have won bets after leaving jars open for 30mins + on the rack and not having any of them continmated. In theory, a fan should work the same way, say a fan underneath a rack, but it doesn't, I've also tested that. Any jar open more than 10mins with a fan under it has been continimated, shrug. I figured I'd post this since I've been doing this for 20 years and never heard/read about anyone using this technique, but it works quite well. I don't see many people innoculating jars with live mycellium either, but using this technique you can innoculate all you like with mycellium and it cuts colonization time down by 4-5 days. In terms you all would understand I get fully colonized 1/2pint PF cakes in 4-5 days. Slightly different mixture than the PF cakes tho. I guess I just need to post my whole method, since I can constantly get mushrooms from spores to fruit in 3 weeks, and anything I've read on the internet has said 6 weeks +. Also, I belive the dosage of PC shown on the internet is a very poor representation of how much you should take. I've seen recommended starting dosages at 2 dried grams. I don't know anyone who thinks 2 dried grams is a good place to start, most people I trip with never do more than 1.5. But this may just be a potency issue? Or it may just be we are old. But even when I was young I never did more than 3 dried grams. I don't even know anyone in 20 years that has done more than 5 dried grams. Anyone that ever wanted to do that much I made them do 3 dried grams first, then asked them if they wanted to do 5. No one has taken me up on it yet;). Ok I guess I'm just rambling now. I'll get bored and type up an entire 3 week method, or at least the good parts.

By Quote (Quote) on Wednesday, February 13, 2002 - 01:58 am

please don't mislead folks by stating that the oven door tek is as good as a glovebox,
it simply is not true,
and causes many newbies grief.

this nonsense should stop here.
oven door tek sux,
it is totally unsuited for any serious sterile work such as liquid inoculants.


Karna's Syringe Tek : Simple Cubie Tek : Lazlo's Syringe Cloning Tek : Spore Print Question : Inoculation Discussion : Psilocyber's Syringe Tek : Dr. Stein's Syringe Tek : Growing Pan Cyans : Inoculating with Filter Disks : Camel's "Advanced" Method : Yeast : Shroom Glossary

By Mr. B (Argonaut) on Tuesday, December 04, 2001 - 05:08 pm:

I have invested enough into my current setup so I really don't want to fuss with a glovebox. Can I get opinions on the open oven-door method as opposed to glove box??


By jared (Jared112) on Tuesday, December 04, 2001 - 05:44 pm:

It is suppose to work good enough if you don't want to spend the money. I never tried it, but I know that when it comes time for me to work with spore prints I'm going to make a glove box. Here is a pic of a very nice and cheap glovebox that Lichen made.

By quote: (Quote) on Tuesday, December 04, 2001 - 05:57 pm:

Build the Cheapest "Glove" Box Possible

Items Required

Cardboard box (13"x15"x10")
Plastic wrap
Duct tape
Dish cloth
Disinfectant spray
Sharp knife or razor blade for cutting cardboard
Rubbing alcohol
No gloves

To raise the odds against contamination, a sterile work area is vital. This is especially true if you want to become independent of outside sources of sterile spore syringes. In a rush I threw together this glove box out of scrounged materials. It has worked so well that I haven't built my dream box yet. First find a clean cardboard box with the above dimensions as a minimum. It doesn't have to be new or pristine, just fairly new with no visible dust or dirt. Cut off the top opening flaps. Cut away the entire front of the box, leaving a 2 inch wide strip of cardboard at the top edge to attach the dish cloth to. Cover the top with plastic wrap and tape down the edges. Using duct tape, attach a dish cloth or towel across the front opening onto that 2 inch strip. The cloth should cover the entire opening of the front of the box like a curtain and you should be able to see into the box through the clear plastic wrap on top. That's it. To use the box, spray down your work surface with the disinfectant spray. Close off all drafts (windows, heater fans, etc). Spray outside of box lightly and saturate the dish cloth. Place bulky items into the box such as grain jars and agar plates. Spray disinfectant into the box until all of the air inside is displaced by the mist. Wash hands with rubbing alcohol and you are ready to work

My First Print Success!

(Oven Tek is flawed, use a cheap glovebox - Nan)

Posted by: Mycota Jan 28 03, 07:42 PM GMT
Eyeball this pic. It speaks for itself. Mycota

Warm air is sweeping particulate into the mouth of the jar!!!