|PF Lids with Honeytek||-||11/07 01:27am|
|Here are a few ideas for liquid tek im working with with pics||7||01/19 12:42am||Pinhead|
|By I feel so weird (Phishgrower) on Friday, October 19, 2001 - 07:42 pm:|
Ok, I understand everything about this tek, except for how to keep the jar lids during the process. Do you just leave the hole in the lid open after you innoculate or do you cover with tape? Leaving it open would introduce bacteria, would it not? please help, thanks.
|By Kaijan (Kaijan) on Friday, October 19, 2001 - 07:52 pm:|
just put some tape back on.
|By Nan (Nanook) on Friday, October 19, 2001 - 07:58 pm:|
I put the jar in the Glovebox and just take the lid off. No holes at all. After I shoot a jar I put a clean, dry, sterile, solid dome lid with no holes, seal side down, over the mouth, screw the band down tight, and replace the foil cap. I presterilize some solid dome lids wrapped in foil when I sterilize the jars. That's a mouthfull...
People use Polyfil, but I will tell you what, it does not work well for liquid culture. Sugar solution inevitably gets into the polyfil, and if a contam starts here it's all over... It will grow through the polyfil in many cases and drop spores inside the jar. Cobweb is especally good at this and it's hard to see, even in dextrose (very tiny black sporebodies)... Ever shoot 30 jars of cobweb?... What a mess... You could easily end up shooting yeast as well without knowing about. Polyfill works great for shooting Grain Tek and 9er Tek. Not good for liquid culture.
In liquid culture you should use solid lids: no holes, no tape, Incubate and store with the Foil Covers on. Keep em clean. Experience talking. Follow the Tek until you are comfortable with honey/dextrose.
|By Brad (Raze) on Thursday, December 13, 2001 - 06:35 pm:|
Ok, I've tried my dextrose jars in many things. My first batch was in an extra 250mL jar (which come only "decorated" around here, making them hard to see through) and it was a pain because I couldnt nuke the jar w/ its lid on, and saran wrap didnt hold while shaking the water about.
My second attempt was in an Absolut Vodka bottle, a 200mL one, worked ok, but the stuff hardly grew for some reason.
My next attempt was in ziplock 1/2 pint containers, they worked well, at first, but they are all warped now from heating to sterilize them (never take the lid off a pc to cool when using these, they shrivel like a frightened turtle) plus the seal wasnt the greatest and my last two were contamed somehow.
My latest attempt was in baby food jars, but that resulted in some sediment, which I now believe was rust off the lid.
So now I need to make up more, since my jars are all almost ready to birth. Any ideas of what I can use for containers?
|By Tripitydoodah (Tripitydoodah) on Thursday, December 13, 2001 - 06:44 pm:|
Brad, i just use a pint size mason jar with an inoculation hole. works great.
|By Brad (Raze) on Thursday, December 13, 2001 - 06:49 pm:|
They would be decorated too
|By quote: (Quote) on Thursday, December 13, 2001 - 09:11 pm:|
fungi perfecti has plastic autoclavable lids for your jars, perfect for microwaving dextrose water.
just search their catalog for 'plastic lids'.
|By ion ewe (Ion) on Tuesday, January 08, 2002 - 04:15 am:|
I don't remember seeing this mentioned in the archives on liquid culture teks...
Seems my buddy forgot about some sugar solutions he had growing from prints. He forgot these things for about 2 weeks after germination. The mycelium grew and died. Looked kinda dark and silty rather than white and clumped. Not real sure when they died, but there's been no growth from jars inoc'd 10 days ago with the water. (He figured he'd try anyway.) Very sad, but it brings up a valid point...
This occurance has incited me to post about how (oh, yeah...duh...) water will suffocate mycelia after a certain time. We're talking longer than twenty-four hours for growing wads of mycelium, but it still happens eventually. My advice: Suck up those syringes as soon as you have enough to go on. If you can't, then put your jars in the fridge to slow down the fungus's metabolism.
Experiment: Oxygenate water with an air stone and an aquarium pump before PC'n. The pressure in the PC should be sufficient not to let the dissolved gases escape much when heated.
One might try a rapid colonization by the same token. You'll need a glove box.
Sterilize the air stone and tubing. (In the glove box, now) Spray the pump with some good iso and put it in a sterile, sealed box (good sealing tupperware or something). Turn the pump to "on" if it has a switch. Affix a hepa filter to a pre-cut hole in this box. Now, run the tubing out (without the stone) from a sealed hole to your jar lid that has a perfect little hole in the center. Run the tubing through and attach the air stone. Inoculate and screw the lid onto the jar (rubber up) loosely. Make sure you run the pump cord (unless it's battery powered) out from a sealed hole in the back!
Now you have a pump with a filtered air source running a slow stream of air into your liquid culture, oxgenating and stirring it. You also have a positive-pressure environment in the jar.
And there was much rejoicing...
I'll write a tek if anyone's interested.
|By quote: (Quote) on Tuesday, January 08, 2002 - 02:56 pm:|
by all means, write it.
and you raise a good point,
i didn't realize our archives omitted that fact that liquid cultures require some air now and then.
that's why i still use polyfil in my lids, even though nan has spoken against it. i keep my liquid level low, and am careful to never get the polyfil wet. but it enables me to just gently swirl the solution every day or two, to oxygenate it.
|By Dr. Cubesis III (Newbieshroomer) on Tuesday, January 08, 2002 - 06:42 pm:|
" I'll write a tek if anyone's interested. "
You just did ION!!! I don't need much more info than that, you made the points you wanted to.
Glad your on our side!
Thanks man! that was great news!
"And there was much rejoicing...
|By plinkerdink420 (Plinkerdink420) on Tuesday, January 08, 2002 - 07:51 pm:|
|By ion ewe (Ion) on Wednesday, January 09, 2002 - 07:48 pm:|
I guess that would do for a tek, huh? I just like to be very specific and organized when writing teks. A material list, numbered steps, etc.
Well, I'll write it on notepad or something and copy it to here. Thanks for the support, guys!