|By trippysmurf on Monday, August 13, 2001 - 05:58 am:|
ok, i promised myself not to hit the panic button till the end of the week, but i was over at the old board, and i was going through old posts, i innoculated 6 jars last week, 3 of the jars are starting to show some cottony growth, i hve micropore tape on the holes and a verm barrier, on one of the threads on the old board it said not to bother with tape but to leave the foil cover on, i took off my foil covering but left the tape on? ive been checking them everyday and was woried that i might be breating germs into my jars, wat do you guys think? sorry, i know this has been answered before but this is my first time and just wanted some reassurance thanks to everyone on the board, i would never have gotten this far without each and everyone of you!
|By Nanook of the North (Nanook) on Monday, August 13, 2001 - 07:39 am:|
Make some place really clean, put the jars in there, and don't bother them. The more they are handled, examined, worried about... The worse things get.
NEVER LOOSEN OR REMOVE THE LIDS FROM INCUBATING JARS!
THEY WILL CONTAM IF UNSEALED
The best is plastic surgical micropore tape, and leave the foil covers on. You can handle a taped, covered jar without much worry of introducing contams. As it is, you will be fine. Put the jars up someplace clean and let them work their magic for awhile.
If you want to do somwething constructive to occupy yourself... Make up some more jars...
Works for me
Slow or Stuck Jars? : Check out Tyvek filter disks!!!
|By Mushy (Mushy) on Monday, August 13, 2001 - 12:57 pm:|
I cover with foil to sterilize jars, no tape. I take foil off when I Innoculate, then cover holes with ordinary masking tape. I leave the tape on til things slow down and most of the top part of the jar looks colonized, then I take off tape and flip.
Last two batches of jars, I have left the tape on the whole time, they colonized so fast, I didn't have to flip them. That was using one syringe per 6 jars, 100% in about 12 days. No contams from post innoculated jars yet...only jar contams I have had have been in the syringe.
|By Mushy (Mushy) on Monday, August 13, 2001 - 08:42 pm:|
Were they taped and covered? the thing to worry about after longish storage time is loss of moisture. I have successfully innoculated and had full colonization of jars that went 3 weeks before innoculation, though they were very dry.
I would go for it, but if you are strapped for cash, you might want to redo the jars, simply because if there is a problem, brf and verm are a lot cheaper than a new syringe.
I seriously wouldn't worry about it after only 7 days, though.
I wouldn't worry, personally...
|By Black Star (Mr_Bug) on Sunday, October 21, 2001 - 04:50 am:|
I have always used black electrical tape, not masking tape. Do you all think masking is better?
|By Brettiejams (Brettiejams) on Sunday, October 21, 2001 - 04:56 am:|
Masking is better than electrical....
But the surgical cloth type is the BEST.
|By Hatcher (Hatcher) on Sunday, October 21, 2001 - 08:52 am:|
Use masking tape. Write your innoculation date, race, anything else right on it. Very practical and easier to tear my man. Straight into the pc w/o aluminum foil too..wuhoo!!
|By Black Star (Mr_Bug) on Monday, October 22, 2001 - 05:22 pm:|
Thanks Hatcher and Brettie! Now, what exactly is surgical cloth type and how can I obtain it?
Also, I once tried to microwave the jars rather than steam them. It failed miserably. I think my problem was that I didn't let them nuke for long enough, and they dried out. You might want to put a large reservior of water in the microwave with the jars and nuke for a long time. The water will dehydrate and saturate the air, but it can't go over 100% humidity, so having a reservior there will probably reduce the amount of drying that takes place.
|By Brettiejams (Brettiejams) on Monday, October 22, 2001 - 06:07 pm:|
surgical cloth tape is the kind of tape you would use to tape a bandage with... you can find it at any drug-store.
The reason it is superior is because it allows for air exchange during the entire colonization process.
It "breathes" but still filters out tiny particles from the air.
If you are gonna use it, this is what I suggest.
Sterilize jars as you normally would(cover with a bit of foil to keep water out).... and then, remove the jars while they are still a bit warm.
While the lids are still pretty warm, but not hot, cover the holes with cloth tape.
The warm jars will still be giving off heat so no airborne particles should be able to settle down onto the the lids while you do this.
Also, the reason to wait to apply the tape until after sterilization is to ensure the adhesive on the tape doesn't get too hot... where it might get soft and clog up your innoculation holes, thus rendering it's "breathing" capacity mute.
|By Black Star (Mr_Bug) on Monday, October 22, 2001 - 06:30 pm:|
Fantastic info Brettie!! Next time it's time for new jars, it's surgical tape all the way!!
|By SYDYSTYK (Addict) on Monday, October 22, 2001 - 06:32 pm:|
its in alot of first aid kits
(might need it sometime in first aid kit tho)
|By Scotsman (Barrowland) on Friday, November 23, 2001 - 05:25 pm:|
My mycelium is growing well,lids are taped but i read jared question about slow growing and he was told remove the tape, should i do the same i would say mine is more than half covered , so tape on or off??????
|By quote: (Quote) on Saturday, November 24, 2001 - 12:06 am:|
i'd remove it, it really isn't needed, and can cause colonization to stall.
|By Scotsman (Barrowland) on Saturday, November 24, 2001 - 01:36 pm:|
tape off .......thanks......
|By greenthumb (Greenthumb) on Saturday, November 24, 2001 - 05:06 pm:|
tape/foil cover talk