|By Anonymous on Wednesday, October 10, 2001 - 09:01 pm: The Nook|
doc i use a variation of
Captain's Oven Method instead of a glovebox to transfer from Print to Syringe.
heat your oven at its lowest setting while you prepare your materials. gather the following materials:
A. 1 small glass or 1/2 pint jar (the smaller the better because spores will stick to the surface area of the glass no matter what you do)
B. a clean plate
C. a razor blade
E. rubbing alcohol
F. cotton balls (or a clean paper towel)
G. spore print (duh)
***-boil water, draw boiling water into syringe, expunge, draw more hot water into syringe, place cap on syringe and allow to cool
I place everything but the bottle of alcohol on the clean plate just to keep it all together and have a semi-sterile place to set everything.
once you have your materials ready turn off the oven and open the door. set the plate on the now opened door or pull out the bottom rack and set your materials on that. the theory is that the hot sterile air escaping out of the oven will carry away any contaminates floating around in your work area.
now splash some alcohol onto your cotton/paper towel and wipe out the inside of the glass, next wipe off the razor blade. pull out your print and scrape into glass w/razor. now wipe off your needle to sterilize and squirt the now cool water into the glass, stir using the needle to break up large clumps of spores and draw the solution into the syringe. place the cap back on the syringe and you're ready to go.
this method is NOT foolproof, but i have been using it for a while now and have never made a bad syringe. knock on wood.
live long and sporulate