Spore Print Question

Nan's Nook : Archives : Spores : Spore Printing : Spore Print Question
  Subtopic Posts Updated Creator
A Spore Print Discussion  64 03/01 08:08pm  
When To Print??? 15 6/03 Voodoo
Dirty prints...  11 12/07 12:14am Purge
Frozen Spores 3 6/03 Maliki
Spore longevity  21 12/07 12:16am saluras
Will Too Many Spores Spoil The Jar?  6 02/14 11:41pm jerkjunky2

By Kevin Smith (Canshroom) on Monday, October 08, 2001 - 06:09 am:

For those of you who have made their own prints...is it as tempermental as the PF Tek makes it sound? I'm almost too scared to try it...lol. Seriously, though, do things have to be that sterile? It seems like it would be almost impossible to achive in the real world.

By Doc Thirst (Doc_Thirst) on Monday, October 08, 2001 - 03:01 pm:

I have never had any real success at it myself. I have tried 3 times, and every atempt has ended badly. I would really like to hear a detailed account of a success story if anyone has one.

By dr. stein (Ipitythefoo) on Monday, October 08, 2001 - 05:58 pm:

it's really not that complicated or difficult of a process. the reason to take extra care when making spore prints is that a good size print will make enough spore solution for 100 jars, or more. if you allow contaminates to invade your spore print then you will be potentially injecting contamination into every jar you innoculate. huge losses, lots of crying, and breaking stuff will ensue.

i just make sure to wash my hands well and flame sterilize the knife i use to cut off the cap. i have a few square pieces of clear glass that i wipe off with rubbing alcohol and set the caps on, then i cover them with clean half-pint jars, wait two days then remove caps and store the glass squares in ziploc bags until i am ready to make syringes.

in the past i have also used notecards and even plain notebook paper, just make sure it's new and clean and cover it while the cap drops its spores.

nanook posted a method involving iodine that sounded good, but i have yet to try - do a keyword search on 'iodine' if you still want more info.


By Doc Thirst (Doc_Thirst) on Monday, October 08, 2001 - 07:23 pm:

What about transfering the spores to syringes? Any advice for safely doing that? For a guy who has had no success with this in the past, what would you suggest to keep the ball rolling, honey? spore prints? syringes?

By Trippysmurf (Trippysmurf) on Tuesday, October 09, 2001 - 06:19 am:

doc, my first time growing i ordered prints, and had to make syringes myself, i haven't tried printing though, but im about to soon, as far as making syringes out of caps go, i would go with the method on the PF site, and use a wire mesh to suspend the cap. from print to syringe i recomend a glove box, thats what i did, you can fumble around in there without worry cause the air is pretty clean although my first time was a bit wasteful, i think its all part of the learning process, if youre growin ur shrooms, no worries, uve a lot of caps to use to keep refining ur technique, just do it man, if you fail, try again, thats about the best thing you can do, its the only hting you can do really, but reading up on it doesnt hurt

By dr. stein (Ipitythefoo) on Wednesday, October 10, 2001 - 08:36 pm:

doc i use a variation of Quote's oven method instead of a glovebox to transfer from print to syringe.

heat your oven at its lowest setting while you prepare your materials. gather the following materials:
A. 1 small glass or 1/2 pint jar (the smaller the better because spores will stick to the surface area of the glass no matter what you do)
B. a clean plate
C. a razor blade
D. syringe(s)***
E. rubbing alcohol
F. cotton balls (or a clean paper towel)
G. spore print (duh)

***-boil water, draw boiling water into syringe, expunge, draw more hot water into syringe, place cap on syringe and allow to cool

I place everything but the bottle of alcohol on the clean plate just to keep it all together and have a semi-sterile place to set everything.

once you have your materials ready turn off the oven and open the door. set the plate on the now opened door or pull out the bottom rack and set your materials on that. the theory is that the hot sterile air escaping out of the oven will carry away any contaminates floating around in your work area.

now splash some alcohol onto your cotton/paper towel and wipe out the inside of the glass, next wipe off the razor blade. pull out your print and scrape into glass w/razor. now wipe off your needle to sterilize and squirt the now cool water into the glass, stir using the needle to break up large clumps of spores and draw the solution into the syringe. place the cap back on the syringe and you're ready to go.

this method is NOT foolproof, but i have been using it for a while now and have never made a bad syringe. knock on wood.

live long and sporulate

[Watch the fire hazard too... Make sure flames and elements are extinguished before introducing alcohol vapors, and use small amounts... Nan]

By Daymoon (Daymoon) on Friday, October 12, 2001 - 12:08 pm:

I've never had a problem making a print PF style. The PF tek for making a print sounds quite exacting for sure...but if reasonably followed, success is not difficult. Believe me, If I can do it anyone can. I spray the room down with disinfectant, get everything set up and wash my hands thoroughly with anti-bacterial soap. Also, I conduct the procedure in my kitchen oven...pull one of the trays out about half way, sterilize a safety pin and stick it in the shroom head for support when I sever the head, and then quickly drop it in the sterilized half-pint jar. Out of many prints, only had one to end up contaminated.

By poplar hill (Itchybake) on Friday, September 21, 2001 - 04:29 pm:

how can you assure that the spores that are collected for a print are sterile? they're coming off a mushroom that has grown in a non-sterile envireonment; that being the fruiting chamber.

By saluras (Saluras) on Friday, September 21, 2001 - 10:12 pm:

the best you can do is be as sterile as possible, when preparing the print.

By ion ewe (Ion) on Saturday, September 22, 2001 - 07:33 am:

You can reduce contamination of the print by doing two things.

1. When you see a big beauty growing, watch it closely. When the upward momentum stops and the veil begins to stretch, put a clear container (like a jar or something else clean) over the whole mushroom. This stops air movement, and with it, flying contams.

2. When you print, don't use the first one. The first layer of spores actually sloughs off over about 6 hours, leaving fresh, clean spores underneath.

Do all of this as quickly and in as clean an environment as possible, and you should have about 70% less contams in your prints. By the way, I pulled that percentage figure right out of my ass. Like a cartoon. Wquo! I've got a bulldozer! Wquo! I've got a mug of soma! Wquo! I'm outta here!


P.S. This really does work. I'm just in a weird mood.

By changling like crazy (Changling) on Saturday, September 22, 2001 - 11:38 pm:

i like to use a seperate growing chamber for one or cakes which will used for printing. a plastic vegetable crisper is good for strains that produce smaller shrooms, and will hold one cake, but there are other alternatives.

By Nan (Nanook) on Monday, October 22, 2001 - 12:40 am:

Materials: Do I use typing paper, wax papar, aluminum foil, 3x5 card, microscope slide, cow tongue?

Wax paper and aluminium foil works good, typing paper works also but a lot of spores are absorbed by the paper

Should I use tape, or shoudn't I?

Use tape

Storage:Cool and dry, cool and damp, hot and dry, hot and damp? Stack verticaly, or file horizontaly? Plastic bags, or no?

Cool and dry, plastic bags..

Time: Leave the cap for one day, or one week?

One day

Cataloging: Just how many strains ARE there realy?

A lot of strains... Comercially available something like 20-25, in the wild probaly thousands...

Maintinance: Re-print each strain every 1 year, 5 years, 10 years?

Every 5 years if well stored

[Prints should be tested every 6 months to a year for germination... Nan]

By C K (Phrozendata) on Tuesday, November 06, 2001 - 05:40 am:

I have a GC cake pinning that I really want to spore print. Some shrooms sporulated during the day and the big one I want to sporulate is about 17 hours after the viel broke. It is not flat, or, very close to it but I realy dont want to miss these spores while I am sleeping. Can I:

Place it on the paper and it will stay alive without the stem to mature and sporulate on the tin foil paer?

Shoud I be worried about my hands and the top of the terrarium that has been touching the top of the cap?

By relic (Relic) on Tuesday, November 06, 2001 - 05:57 am:

you better wait it out as long as possible. even if it starts to drop them a bit before it's plucked you'll still get tons of spores. it will live a bit after it's picked. i'm not sure how you take prints, but, i use glasses upside down on paper towels or inside clean tupperware. it helps if one is a bit early to lightly mist the inside of the glass or lid. a word of warning, once i had a huge pfmr cap i was going to print and i picked it a bit early, bbout like yours, and figured it would open the rest of the way within another 24 hrs. and it never did after i cut it.

By Nan (Nanook) on Tuesday, November 06, 2001 - 06:10 am:

Yeah Relic I have had the same thing happen here. Don't pick the cap until it starts printing good. Time is on your side.

Fully mature shrooms in the fruiting chamber will drop spores for days... Heavy. I have seen chambers printed so dark it looked like a toner cartridge exploded in there.

Caps start printing lightly and gather up steam as they get older. The longer you can hold off, the better the print will be... And you will know when it's ready... You will see spores, then wait 12 hours... Even longer. Get a good night's sleep.

Don't take first flush prints. First flush shrooms print weaker than later flushes in my experience.

By serialkiller1 (Serialkiller1) on Monday, November 19, 2001 - 04:33 am:

when is the best time to pick the mushrooms for spore prints. when is the best time to pick for ingestion?

By Lichen (Lichen) on Monday, November 19, 2001 - 05:54 am:

for eating, pick them when the veil starts to tear away. For printing, wait till you see spores on the stipe or under the shroom on the perlite or on the cake or casing. Once the spores have begun to drop, is the perfect time.

By Liberty_Caps (Liberty_Caps) on Wednesday, November 21, 2001 - 03:26 am:

I'm curious....
why do people say NOT to take prints off the first flush?????
I want to make some prints but If I take prints off the first flush what will be wrong with them???
is this true???

By quote: (Quote) on Wednesday, November 21, 2001 - 02:18 pm:

they'll be ok for printing,
not sure why some advise against it.
nan said that later flushes often print darker,
but don't let that stop you,
first flush prints can be pretty dark, too.

By Nan (Nanook) on Wednesday, November 21, 2001 - 05:11 pm:

Second flush prints are usually significantly higher quality in my experience. The spores seem more potent too. I have printed PF and Albino strains from cakes where I could hardly get a shadow printed from the first flush. The second flush shrooms just seem to print better.

I agree, if you have a first flush shroom that is mature and dropping... print that puppy.

Hawaiians will dump spores like a busted toner cartridge sometimes, even on the first flush. It's a good printing strain.


By ralph (Ralphster44) on Friday, November 23, 2001 - 05:54 pm:

Normally, you would pick your first flush to eat.
You dont want the shrooms sporulating on
your casing layer.

Thats why people will take their prints
after the first flush.

I finally made it here..Hello everyone!!

By An guy (Boomer) on Tuesday, December 04, 2001 - 06:42 am:

When taking a print?

Been on glass now for over 24 hours, still fleshy, not deliquescing any that I can see. Think they're still throwing, but I'm not sure.

Should I be for taking them out now, or leave them longer?

Thanks for your support....;)

And thanks for talking me through last night when I was putting them in the jars...

By relic (Relic) on Tuesday, December 04, 2001 - 06:47 am:

look underneath. if it's dropping spores when printed, 24 hours is usually enough to get a good dark print. you can see the print around the upturned edges of the cap and pretty much tell how dark it is w/o lifting or opening the jar or container.

By jared (Jared112) on Tuesday, December 04, 2001 - 04:27 pm:

Is it bad to tap the cap with your finger or shake it while its in the jar to drop more spores?

By Nan (Nanook) on Wednesday, December 05, 2001 - 08:01 am:

Spores don't accumulate on the gills. It's not like there is a pile of spores, like dried seeds, waiting to get knocked off the gills.

The gills are alive and as spores are produced that are sloughed off the gill surface. There is nothing to knock free.

Leave to poor cap in peace.


Posted by: groingrinder May 21 03, 01:25 AM GMT
I have a cap from the Gulf Coast strain that I cut off when it started turning up. I then placed it on al foil inside of a half pint wide mouth jar. That was four days ago and still no spores. Anybody else have this experience? ohmy.gif

Posted by: steveoi812 May 21 03, 02:34 AM GMT
It seems like some caps want to drop more than others. I have had this problem. I guess you have to pic them at just the right time. These south americans my friend has are making the darkest prints I have ever seen before.

Posted by: Nanook May 21 03, 02:54 AM GMT
Some caps never drop, other caps will drop non-viable spores (this one stumped me)...

1) Wait until you can verify the cap is producing spores before you print it. Never trust a cap that is not producing spores when you pick it to produce a print. Ideally the area under the cap should look like someone broke a toner cartridge in your fruiting chamber... You see that and you know your prints will be heavy.

2) If the print is important... test germinate a sample to make sure the spores are good... I have had caps that printed non-germinating spores.

Posted by: groingrinder May 21 03, 04:03 AM GMT
Thanks for the tips folks. wub.gif


Posted by: scotsman May 23 03, 04:41 AM GMT
to me picking the right shroom for printing is 50\50
50 luck and 50 skill smile.gif

how long would you think you could leave a shroom
dropping spores before it starts to contam?

Posted by: Nanook May 23 03, 11:59 AM GMT
Spore Printing