Cloning in Honey

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By Timothy Leary (Timothyleary) on Monday, October 08, 2001 - 03:48 am:

I was wondering if it is possible to clone in honey water. or if not clone(using actual tissue), then could one possibly take a small portion of mycelium from a cake, drop it in a sterlized honey/h2o solution?

i apologize if this or simliar questions have been ansered before. I have not seen any in the recent posts (last month or so).

Any thoughts are welcome, but simple yes/no answers with no explinations are fine if no one has the time.


By Nanook of the North (Nanook) on Monday, October 08, 2001 - 06:53 am:

It's OK. It's a good question.

The way I do it is to take a tissue sample from the stipe and blend it per 9er Tek. Then I shoot jars of liquid substrate, grow it out 3 days at 87*F in an incubator, and use the liquid inoculum to shoot jars. The liquid inoculum can be stored for up to one year in the fridge.


By Timothy Leary (Timothyleary) on Monday, October 08, 2001 - 05:09 pm:

Let's think about this:

If you could use a piece of mycelium in honey water, every jar can make a whole bach of honey water without compromising its fruiting at all.

One batch of honey water per jar, ten jars in one syringe, one syringe would last you along time.

I think i should call it the super poor man's tek. Unless there is a tek like it? if not, i am starting experiments.

By Marx2k (Marx2k) on Wednesday, October 10, 2001 - 07:54 pm:

Also, you can add up to 5ml Hydrogen Peroxide AFTER sterilizing the honey water to ward off any contaminants that may be lurking around.

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