Growing invitro?

Nan's Nook : Archives : Jars : Growing invitro?
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By Fanaticus (Fanaticus) on Wednesday, November 07, 2001 - 05:50 pm:

Here is a photo of invitro PF shrooms grown in a drinking glass.

These shrooms are actually the best because they are primordia with no spores yet. They are "ugly" but they look clean and uncontaminated. They are the ones for tripping, but lousy for asthetic growing.

invitro2.jpg

PF

By An guy (Boomer) on Wednesday, November 07, 2001 - 06:25 pm:

and your PF strain is one of the best for invitro growing, am I correct?

I want to thank you, btw- I have been poring over this board, and thinking about your original tek and this and that- seems there'r things in there that have gone by the way side for new things- and seems some of that is good, but some of your original stuff might be good to bring back up and review-

For instance- you advocated growing at room temp- around 75 degrees.

It dawned on me one day, when my area was really warm- I panicked, wondering what might have taken root- I instinctively knew that was bad vis a vis new contams.

So, and other people have made comments to back those feelings up. So maybe use two temps- like Lichen having trouble with cake contams- maybe colonize some at lower temps, see what happens, see of they don't do better, albiet slower.

Wow did I get off track-

What I really wanted to say is no matter how far along branching paths people have gone beyond your basic tek, I think you're the one, basically, who put it all together, and put it in peoples homes, such that we now have boards like this. That's a big deal, and thank you.

By Lichen (Lichen) on Wednesday, November 07, 2001 - 11:44 pm:

incubation should be at around 80-85F, while fruiting should be at 75F. And while I usually do not have cake contams, I just pulled one that had trich...it's the jars that have been going south. And the damn straw. But I think I'm getting a handle on it. Outside contams had been getting in there on the feet of the fruitflies. Now that I have been keeping them out, I haven't had a problem, yet

By Fanaticus (Fanaticus) on Thursday, November 08, 2001 - 11:48 am:

The pic was from Yashic (of Holland - PFE fame)
It was completely cultivated inside the glass and the lid was removed and the jar put in a terrarium so that the primordia on the top could grow. If let be, the top primordia will turn into a very nice shroom, but the fruiting is ready for harvest.

My friend from Holland tries everything and is not afraid to try "crazy" stuff too.

He says that INVITRO rules for easy growing and he grows some nice flushes regular pf style also.

Thanx for putting the photo under the invitro tek.

The trick is to just watch the jar or glass for puddling and I like to suck out the puddling water with a thin straw or super long syringe needle (blunted for safety). Puddling seems to be the only real problem of invitro growing but it is very easy to remedy. All one has to do is watch the glass and suck out the puddled water.

PF

By quote: (Quote) on Thursday, November 08, 2001 - 02:30 pm:

hey, that's a pretty good idea.
i just usually open the jars and wipe out excess water, but your method sounds faster .
thx, pf.

By whoever (Livedangerous) on Tuesday, October 02, 2001 - 12:21 am:

what kind of results should one expect from growing invitro? im also not completely clear about this tek, do you let it grow the full term(6 weeks or whatever)? im growing mazatec, hawaiian, pf, and false mexicans. do any of these species benefit a great amount from this tek. what about Lighting for invitro?


[Ed. Note: Invitro is a stealth Tek. It's not much fun to fruit.]

By Liberty_Caps (Liberty_Caps) on Tuesday, October 02, 2001 - 12:25 am:

PF is the best race for inverto. and yes u need light, it vareys on how much you will get out of inverto

By Lichen (Lichen) on Wednesday, October 03, 2001 - 01:06 am:

Hawaiian, PFclassic, and false Mex all will do fine invitro. Give them indirect light after they start pinning (invitro primordiation)

By Mr. Tambourine Man (Tambourine_Man) on Tuesday, November 13, 2001 - 01:01 am:

Hi, I read about the double casing tek where vermiculite is placed both at the top and bottom of the jar. Will this work for the Invitro tek where the cake is never birthed or cased? Also, I have read that Ball half-pint tapered jars will work for PF tek but will they work for the Invitro tek just as well as wide mouths? Thanks a lot!

By quote: (Quote) on Tuesday, November 13, 2001 - 01:07 am:

yeah, just put a thin layer of verm. in the bottom of the jar before loading in the rice/verm mixture for sterilization, that way you're double end cased invitro right from the start.
the tall tapered will work, but they can be difficult come harvest time to get the cake out, a few good firm raps on a hard surface helps.

By Mr. Tambourine Man (Tambourine_Man) on Tuesday, November 13, 2001 - 01:11 am:

Thanks Quote, I appreciate it!

By koolaid (Koolaid) on Saturday, November 17, 2001 - 07:38 am:

Hello everyone, I'm about to grow invitro. I have a couple of questions. Can all Cubes be grown successfully and abdundantly using this method? (If this has already been posted, I apologize for overlooking it). Also, I know that water can pool up on the bottom of the jars. How do you remedy this problem? I've heard of using a straw, but I don't want suck that nasty water in my mouth.

By koolaid (Koolaid) on Saturday, November 17, 2001 - 07:40 am:

Oh yeah I forgot one more thing. I'm guessing that since the shrooms don't grow to full maturity that you can't get spores from them. So, how do you reproduce these babies, cloning?

By Nan (Nanook) on Saturday, November 17, 2001 - 07:56 am:

I don't have much Invitro experience. Other's will post behind me I am sure.

PF Classic is the best shroom for Invitro. When water pools, tip the jars and let the water run along the glass until it is absorbed by the Verm barrier.

You can birth Invitro jars, let the shrooms stand up, open up, and sporulate, You can also clone excellent cakes. I would propose cloning.

By quote: (Quote) on Saturday, November 17, 2001 - 01:21 pm:

unfortunately, some strains are pretty reluctant to fruit invitro, for example, thai and cambodians..
most do ok, like hawaiians or ecuadors,
and a few excel, such as pf and australian or gulf coast.
some, like mazatec, i haven't tested yet.
once the cake is fully colonized, you can open the jar and clean out excess water as needed.
if you want spores invitro, simply transfer the cake to a quart jar.
invitroquart

By Nan (Nanook) on Saturday, November 17, 2001 - 01:27 pm:

Like I said... Thank's for the backup "Quo", it was a good shift. I am off to catch some ZZZZzzzzsssss

By koolaid (Koolaid) on Sunday, November 18, 2001 - 04:56 am:

THANKS guys for answering my questions. I couldn't of asked for any better experts on the subject of growing. It kinda sucks that not all Cubes can be grown Invitro. I was planning on growing Puerto Ricans, Pink Buffalos(Thai), PESA, and Ecuadors-which is about the only one that will work out of my selection. Oh yeah, I was going to put about 2 dozen jars or so in a Rubbermaid container and have a flourescent light above this. Is this all I need? I think so, just need a little reassurance.

By Nan (Nanook) on Sunday, November 18, 2001 - 05:44 am:

Yeah that will work fine for Invitro.

By pack rat dat (Packrat) on Sunday, November 25, 2001 - 08:45 pm:

I have 18 jars fully colonized using the neglect tek and I am wondering if the jars need to stay warm through the fruiting cycle?? thanks

By Cragith Kilbonith (Kilborn) on Sunday, November 25, 2001 - 09:04 pm:

long as the room temp aint 60* try to keep the jars 75*. iv never done the neglect tek but im assuming its the same as pf tek.

By quote: (Quote) on Monday, November 26, 2001 - 02:33 pm:

they'll grow faster when kept warm,
but they'll still grow even at cool temps,
eventually.
aim for about 75*F.

By potomac (Potomac) on Wednesday, November 21, 2001 - 05:46 am:

I started this hobby 8 months ago and I still donít have anything to show for it. Iím still trying though. I have a few questions. I got my eq from Ralph a month ago. I inoculated 12 quart jars in total. 3 of them were filled with %4 malt solution. 9 of them were filled with %80 millet %20 earth worm castings. After one week I inoculated 36 more jars with the living malt solution. After three weeks the 9 quart jars and 36 quart jars are completely colonized with no contamination. None of the jars are tapered so I canít dump out the solid chunk of mycelium. I can still shake them though.

1. The first 9 jars were started from spores. Must I case them separately. Should I remove the spawn from the jars to other containers? Can I case them without taking them out of the jars?
2. The second 36 jars were inoculated from living malt solution. Must I case them separately. Should I remove the spawn from the jars to other containers? Can I case them without taking them out of the jars?
3. Would it be better if I emptied the contents of each jar into its own form?
4. Is the liquid malt solution compatable? Do the 36 jars have the same type of mycelium growing in them or are they all different? Can I mix them together?
5. Just got a couple bales of straw. I sure would like to inoculate it with the 36 jars that were started from the liquid culture. Is 10-20 percent a good spawn to straw ratio?
6. I have six translucent Rubbermaid storage bins
7. What do you think? Please offer any teks you think would help me out.

Thanx in advance,
Potomac

By SYDYSTYK (Addict) on Wednesday, November 21, 2001 - 06:02 am:

im sorry i cant answer your post
but i have a ton of admiration for 9 months of patience, try the pf tek exactly to the letter it worked the first time for me

PF=guaranteed results(nothing is guaranteed, but i guarantee it:)

By jim brown (Shrhobbyist) on Wednesday, November 21, 2001 - 06:15 am:

The answer to each of your question is; forget it and start over. If you are a beginer, than the tek you describe is practically guaranteed failure. Go with the PF tek (Fanaticus Web Site) for your first 5 times at least. You should not even try to mess with that stuff you tried until you have grown a dry pound or so. The number one cause of newbie failure is trying to get ahead of yourself.

By Nan (Nanook) on Wednesday, November 21, 2001 - 06:16 am:

>> After three weeks the 9 quart jars and 36 quart jars are completely colonized with no contamination. None of the jars are tapered so I can't dump out the solid chunk of mycelium. I can still shake them though. <<

Wow... Here we go guys...

It sounds to me like you started this entire germination from a single sterile sporeprint from Ralph... So we are only dealing with one strain. So all of your mycelium is compatable. It's all EQ from a single print, you have no worries here.

Straw is ambitious. You should try to fruit most of these jars directly from the grain. You have lots of choices:

Pie Pan Casing Tek; Quart Jar Invitro; most any Casing Tek

By jim brown (Shrhobbyist) on Wednesday, November 21, 2001 - 07:10 am:

Whose Ralph? Is it a spore vendor or some sort of tek?

By Nan (Nanook) on Wednesday, November 21, 2001 - 07:27 am:

Spore Vendor/Trader of high repute. Very good prints.

By jim brown (Shrhobbyist) on Wednesday, November 21, 2001 - 07:38 am:

Where can I find his info?

By quote: (Quote) on Wednesday, November 21, 2001 - 03:01 pm:

last address i had for ralph was
http://www.geocities.com/ralph1956_2000/ralphster44_FSR.html?988942758830

as for the questions,
yes, you can case right in the jars.
here's an example.
oss

By quote: (Quote) on Wednesday, November 21, 2001 - 04:44 pm:

btw, you'd probably want to wrap those jars with black plastic to prevent the formation of shrooms down where you can't pick.

By ralph (Ralphster44) on Friday, November 23, 2001 - 06:00 pm:

Good advise as usual Quo :)

By Digital-Junkie (Digitaljunkie) on Tuesday, November 27, 2001 - 01:17 pm:

Hey Quote, could you give me some details about casing in the jars?
This sounds promising..

Digital.

By quote: (Quote) on Tuesday, November 27, 2001 - 06:41 pm:

nothing to it.
just add about a half inch of casing on top,
mist it a bit,
and place into the terrarium.
give it light, humidity about 75-80%,
air exchanges 2-3 times daily,
temp about 75-80*F.
then just wait...

Shroom Glossary

By Mr. Tambourine Man (Tambourine_Man) on Sunday, December 16, 2001 - 01:53 am:

Hi, after some picking, should a cake be returned to its jar (Invitro tek) with fresh vermiculite on its top and bottom that is wet or dry? Thanks!

By quote: (Quote) on Sunday, December 16, 2001 - 02:40 am:

damp.

By Mr. Tambourine Man (Tambourine_Man) on Sunday, December 16, 2001 - 02:20 am:

What do you guys think? Should a quart jar be sterilized is a half-pint mid-flush cake is being birthed to it?

By quote: (Quote) on Sunday, December 16, 2001 - 02:32 am:

no real need.

By Mr. Tambourine Man (Tambourine_Man) on Sunday, December 16, 2001 - 03:28 am:

How does it stay humid enough inside the quart jar? It just does?

By Brettiejams (Brettiejams) on Sunday, December 16, 2001 - 03:51 am:

The cake holds it's own moisture, but you could always put some wet perlite in the bottom of the lid.

By God O Mushies (Toadstool_God) on Monday, December 17, 2001 - 09:26 pm:

If anyone especialy quopy has any pics of the Mycro/quopy neglect tek" in action please post em or post the link. They would be really helpful. Thanks

By quote: (Quote) on Tuesday, December 18, 2001 - 12:39 am:

invitro1
invitro2

By Mr. Tambourine Man (Tambourine_Man) on Tuesday, December 18, 2001 - 02:30 am:

Which strain Quote?

By Stewie (Stew) on Tuesday, December 18, 2001 - 03:32 am:

Are those tall half pints? If they are I hope mine will soon look like that.

By quote: (Quote) on Tuesday, December 18, 2001 - 09:02 pm:

i don't remember what he said the strain was.

By Fishy1 (Fishy1) on Wednesday, December 19, 2001 - 01:08 am:

I have found that if you do maybe 1 or 2 jars in a dozen only filled 1/2 way up with substrate, and use no vermic. barrier, you can get a few to poke up to the empty space and open normally.--that way you can get prints, and make your own syringes. This makes it even more stealth. No more paranoia in ordering prints/syringes, and no more fear that mom will thing you are getting mailorder heroin!!
Just a tip....kinda off topic. fishy1

By God O Mushies (Toadstool_God) on Thursday, December 20, 2001 - 06:23 pm:

I ordered Mazateca and Pf from the Pf a little while ago. Waiting on em. Are these two species good for the neg tek? Oh..and by exposing them to light..can I just use an ordinary house buld for the light?

By quote: (Quote) on Thursday, December 20, 2001 - 06:27 pm:

haven't tried the maz yet, mine are coming from pf as we speak.
but i'm confident they'll work, just expose them to light from day 1.
the pf's do great, but i'd definitly dunk them once they colonize fully, they really need the extra water to flush well.
an ordinary house bulb will work fine.

By Stewie (Stew) on Friday, December 21, 2001 - 01:50 am:

shit I thought you werent suppose to dunk until after a flush or two for invitro.

Its been 10 days since my first pins and I only have 5 or so mushrooms over an inch long. Btw I have 24 jars. I have never grown invitro and was wondering if this was slow.

I was wondering if I should clean and dunk or just hang in there a little longer

By quote: (Quote) on Saturday, December 22, 2001 - 12:05 am:

clean & dunk, they need the water by now.
what's the temp?

By ggg (Ggg) on Friday, December 21, 2001 - 01:29 pm:

Anybody try full pint invitro growth?
Anyone try invitro growing with mixes other than strait PF BRF\verm mix?

Thanks for help and Happy Quanza and the like-

piece

By plinkerdink420 (Plinkerdink420) on Friday, December 21, 2001 - 02:10 pm:

i am currently trying it with an alien substrate in a pint jar... only half pint cake though... it is fluffing up real nice.. i'm just waiting for the pins

By quote: (Quote) on Friday, December 21, 2001 - 11:33 pm:

it can be a real ordeal trying to get invitro fruit out of a pint. they grow fine, you just can't get them out.

By ggg (Ggg) on Saturday, December 22, 2001 - 01:55 pm:

I found a place that sells Ball wide mouth tapered pints. They are shaped just like the the tapered halves. Are these the jars you had trouble with Quo?

By quote: (Quote) on Saturday, December 22, 2001 - 04:20 pm:

not i, others.
i use the short fat 1/2 pints .

By ggg (Ggg) on Sunday, December 23, 2001 - 12:01 am:

thanks quo
1 contamed pint = 2 contamed 1/2's
I would call that a risk versus reward thing.

I would need to see a reward in the dry grams yielded per cu. ft. of space used- to say that the compartmentalization against contams with the 1/2's is worth ditching in favor of less innoculation labor with the full pints.

Half pints are good for Quo
They should work for me, just fine.

Quo Questions--

How about invitro substrate recipes?

I know you like the classics on BRF. Have you worked with anything that outperforms them?

Have your invitro yields, on the whole, remained as good as they have been relayed to us on the PF site?

Thanks for the learned guidence

G

By quote: (Quote) on Sunday, December 23, 2001 - 03:14 pm:

i use pf's max formula.
other strains that do as well or better than pfc's include; australians, gulf coast, and south americans.

By God O Mushies (Toadstool_God) on Monday, December 24, 2001 - 05:20 am:

Quote, still waiting on my damn syringes but its cool..Im patiant*twitch* Anyway from what you said I need to dunk the pf cakes once fully colonized right? How about the mazateca's? Does pre-flush dunking work on all races? And Ive heard colonazation can fully occur at three weeks then the pinning invitro begins..do I dunk the cake before it begins to pin? Damn I can barely wait for my syringes..got EVERY other darn thing but them. Thanks quo!

By quote: (Quote) on Monday, December 24, 2001 - 03:43 pm:

haven't grown mazs yet, i'm still waiting for my syringes of it, like you.
any race can benefit from a pre-flush dunk, but pf's definitly need it because it creates so many pins that much more water is needed, or they'll all abort.
you can dunk with small pins present safely, but do it b4 they get very large.