Substrate Colonization Question?

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By Tree Root (Tree) on Thursday, September 27, 2001 - 09:25 pm:

This site is really helpful....Anyway. Eleven days in since innoculation and I went overboard with sterile aspects, surgical mask, surgical gloves, bleached entire apartment, flamed the needle, pressure cooker, the works. I have 50% colonization now on PF tek rice cake substrate recipe. Looking good I think? I live in the northeast and at night it is dropping to 45 and during the day it is 75. The growing area around the jars is 70. I find the substrate colonizes the fastest on rainy days but colonizing began on the third day after I stood the jars in 1/8 inch of sterile water in a half hotel pan and wrapped that sucker with plastic and beamed two desk lamps on them with the jar lids loosened. Okay the questions now.

1.How do I speed up colonization?
2.Should I loosen jar lids to allow oxygen in? Is this good or bad?
3.How can I tell if substrate is too dry?
4.If too dry are there remedies to fix this?
5.Am I being too paranoid and should not fuck with them?

I have been reading all these incredibly fast colonizing stories and I'm worried these are going to slow. Sorry for being so longwinded and verbose. I just want these babies to be done for halloween. -Tree-

By Brettiejams (Brettiejams) on Friday, September 28, 2001 - 12:09 am:

Best way to speed up colonization is to up the temp to around 85 degress.... no higher than 90 though.

I wouldn't loosen the jar lids... the holes you used to innoculate through will provide for enough gas exchange. At 50% colonization you can take the tape off... I like to keep the jars inverted after that.

As long as the mycellium continues to grow the substrate is fine.(water wise)...besides there is really no good way to adjust water content in the substrate once it is already in the jars anyway.

Patience my son.... temp is the main factor in the speed of colonization.

By Tree Root (Tree) on Friday, September 28, 2001 - 06:51 pm:

Thank you Brettiejams. Before I got your advice I went ahead and wrapped the jars again in plastic wrap and this increased the temp to 82. Came back this afternoon and at least a 30% increase in colonization from yesterday. I would say jars ar now at 75% colonized....Yahoo!!

Do you have any tips for Invitro Fruiting a.k.a (quote tek method) or should this be avoided?

By Tree Root (Tree) on Friday, September 28, 2001 - 07:13 pm:

Oh yeah Brettiejams, added again one 1/8 inch of sterile h20 to half hotel pan before wrapping plastic around them. Relative humidity in my region is very low. I find this is the trick creating a humid environmen. I change water, bleach pan and rinse daily to prevent any build up of nasties. Like a poormans greenhouse I suspect. Thanks for the input again.

Can you predict yield ratios of fruiting or is there too many variables to do so?

By dr. stein (Ipitythefoo) on Friday, September 28, 2001 - 08:18 pm:

there are indeed many variables that factor into fruiting Root. the main five factors are humidity, air exchange, humidity, strain, and humidity. with my old hand spraying method i used to get about 1-3 grams dry per cake per flush (pf strain). since switching to a totally air-tight shelving system w/ultrasonic humidifier about a year ago (and changing to hawaiian & equador strains) i now get about 3-6 gms from each.
at the rate you're going i would say you'll definitely have some little funguys by fright night. good luck.

By Brettiejams (Brettiejams) on Friday, September 28, 2001 - 09:48 pm:

TreeRoot... No need to increase the humidity in the air around the jars... the jars have a micro-climate in and unto themselves INSIDE the jar. Just keeping the jars warm will heat the air and water already in the jars and create a nearly perfect growing enviornment.

Now if you are keepeing the water warm around the jars to keep the jars warm then that is fine too.

I have always prefered fruiting the cakes in a terrarium the traditional way, but if you have any specific in-vitro questions I'd be glad to answer